LOCUS       sequence-205773-       11991 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FR cassette provider to introduce FR cassette to a BAC by 
            recombineering.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11991)
  AUTHORS   Sima J, Bartlett DA, Gordon MR, Gilbert DM
  TITLE     Bacterial artificial chromosomes establish replication timing and 
            sub-nuclear compartment de novo as extra-chromosomal vectors.
  JOURNAL   Nucleic Acids Res. 2018 Feb 28;46(4):1810-1820. doi: 
            10.1093/nar/gkx1265.
  PUBMED    29294101
REFERENCE   2  (bases 1 to 11991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-02-28- 28"; volume: "46"; issue: 
            "4"; pages: "1810-1820"
COMMENT     Sequence Label: pDY-HA1-HA2-neo
FEATURES             Location/Qualifiers
     source          1..11991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             385..2310
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGIALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPPPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     polyA_signal    5183..5231
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5256..5275
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     promoter        complement(6347..6492)
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     promoter        complement(join(7834..7839,7840..7856,7857..7862))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     protein_bind    7891..7924
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(8157..8745)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8237..8256)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10074..10573
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10109..10132)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10506..10525
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        10585..10632
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10651..11454
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10714..10733)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11324..11343
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11486..11503)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11492..11716
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     11927..11945
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gatcctcaca ggccgcaccc agcttttctt ccgttgcccc agtagcatct ctgtctggtg
       61 accttgaaga ggaagaggag gggtcccgag aatccccatc cctaccgtcc agcaaaaagg
      121 gggacgagga atttgaggcc tggcttgagg ctcaggacgc aaatcttgag gatgttcagc
      181 gggagttttc cgggctgcga gtaattggtg atgaggacga ggatggttcg gaggatgggg
      241 aattttcaga cctggatctg tctgacagcg accatgaagg ggatgagggt gggggggctg
      301 ttggaggggg caggagtctg cactccctgt attcactgag cgtcgtctaa taaagatgtc
      361 tattgatctc ttttagtgtg aatcatgtct gacgaggggc caggtacagg acctggaaat
      421 ggcctaggag agaagggaga cacatctgga ccagaaggct ccggcggcag tggacctcaa
      481 agaagagggg gtgataacca tggacgagga cggggaagag gacgaggacg aggaggcgga
      541 agaccaggag ccccgggcgg ctcaggatca gggccaagac atagagatgg tgtccggaga
      601 ccccaaaaac gtccaagttg cattggctgc aaagggaccc acggtggaac aggagcagga
      661 gcaggagcgg gaggggcagg agcaggaggg gcaggagcag gaggaggggc aggagcagga
      721 ggaggggcag gaggggcagg aggggcagga ggggcaggag caggaggagg ggcaggagca
      781 ggaggagggg caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggagga
      841 ggggcaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc aggagcagga
      901 ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg
      961 gcaggagggg caggagcagg aggaggggca ggagcaggag gggcaggagg ggcaggaggg
     1021 gcaggagcag gaggggcagg agcaggagga ggggcaggag gggcaggagg ggcaggagca
     1081 ggaggggcag gagcaggagg ggcaggagca ggaggggcag gagcaggagg ggcaggaggg
     1141 gcaggagcag gaggggcagg aggggcagga gcaggagggg caggaggggc aggagcagga
     1201 ggaggggcag gaggggcagg agcaggagga ggggcaggag gggcaggagc aggaggggca
     1261 ggaggggcag gagcaggagg ggcaggaggg gcaggagcag gaggggcagg aggggcagga
     1321 gcaggaggag gggcaggagc aggaggggca ggagcaggag gtggaggccg gggtcgagga
     1381 ggcagtggag gccggggtcg aggaggtagt ggaggccggg gtcgaggagg tagtggaggc
     1441 cgccggggta gaggacgtga aagagccagg gggggaagtc gtgaaagagc cagggggaga
     1501 ggtcgtggac gtggagaaaa gaggcccagg agtcccagta gtcagtcatc atcatccggg
     1561 tctccaccgc gcaggccccc tccaggtaga aggccatttt tccaccctgt aggggaagcc
     1621 gattattttg aataccacca agaaggtggc ccagatggtg agcctgacgt gcccccggga
     1681 gcgatagagc agggccccgc agatgaccca ggagaaggcc caagcactgg accccggggt
     1741 cagggtgatg gaggcaggcg caaaaaagga gggtggtttg gaaagcatcg tggtcaagga
     1801 ggttccaacc cgaaatttga gaacattgca gaaggtttaa gagctctcct ggctaggagt
     1861 cacgtagaaa ggactaccga cgaaggaact tgggtcgccg gtgtgttcgt atatggaggt
     1921 agtaagacct ccctttacaa cctaaggcga ggaattgccc ttgctattcc acaatgtcgt
     1981 cttacaccat tgagtcgtct cccctttgga atggcccctg gacccggccc acaacctggc
     2041 ccgctaaggg agtccattgt ctgttatttc atggtctttt tacaaactca tatatttgct
     2101 gaggttttga aggatgcgat taaggacctt gttatgacaa agccccctcc tacctgcaat
     2161 atcagggtga ctgtgtgcag ctttgacgat ggagtagatt tgcctccctg gtttccacct
     2221 atggtggaag gggctgccgc ggagggtgat gacggagatg acggagatga aggaggtgat
     2281 ggagatgagg gtgaggaagg gcaggagtga tgtaacttgt taggagacgc cctcaatcgt
     2341 attaaaagcc gtgtattccc ccgcactaaa gaataaatcc ccagtagaca tcatgcgtgc
     2401 tgttggtgta tttctggcca tctgtcttgt caccattttc gtcctcccaa catggggcaa
     2461 ttgggcatac ccatgttgtc acgtcactca gctccgcgct caacaccttc tcgcgttgga
     2521 aaacattagc gacatttacc tggtgagcaa tcagacatgc gacggcttta gcctggcctc
     2581 cttaaattca cctaagaatg ggagcaacca gcatgcagga aaaggacaag cagcgaaaat
     2641 tcacgccccc ttgggaggtg gcggcatatg caaaggatag cactcccact ctactactgg
     2701 gtatcatatg ctgactgtat atgcatgagg atagcatatg ctacccggat acagattagg
     2761 atagcatata ctacccagat atagattagg atagcatatg ctacccagat atagattagg
     2821 atagcctatg ctacccagat ataaattagg atagcatata ctacccagat atagattagg
     2881 atagcatatg ctacccagat atagattagg atagcctatg ctacccagat atagattagg
     2941 atagcatatg ctacccagat atagattagg atagcatatg ctatccagat atttgggtag
     3001 tatatgctac ccagatataa attaggatag catatactac cctaatctct attaggatag
     3061 catatgctac ccggatacag attaggatag catatactac ccagatatag attaggatag
     3121 catatgctac ccagatatag attaggatag cctatgctac ccagatataa attaggatag
     3181 catatactac ccagatatag attaggatag catatgctac ccagatatag attaggatag
     3241 cctatgctac ccagatatag attaggatag catatgctat ccagatattt gggtagtata
     3301 tgctacccat ggcaacatta gcccaccgtg ctctcagcga cctcgtgaat atgaggacca
     3361 acaaccctgt gcttggcgct caggcgcaag tgtgtgtaat ttgtcctcca gatcgcagca
     3421 atcgcgcccc tatcttggcc cgcccaccta cttatgcagg tattccccgg ggtgccatta
     3481 gtggttttgt gggcaagtgg tttgaccgca gtggttagcg gggttacaat cagccaagtt
     3541 attacaccct tattttacag tccaaaaccg cagggcggcg tgtgggggct gacgcgtgcc
     3601 cccactccac aatttcaaaa aaaagagtgg ccacttgtct ttgtttatgg gccccattgg
     3661 cgtggagccc cgtttaattt tcgggggtgt tagagacaac cagtggagtc cgctgctgtc
     3721 ggcgtccact ctctttcccc ttgttacaaa tagagtgtaa caacatggtt cacctgtctt
     3781 ggtccctgcc tgggacacat cttaataacc ccagtatcat attgcactag gattatgtgt
     3841 tgcccatagc cataaattcg tgtgagatgg acatccagtc tttacggctt gtccccaccc
     3901 catggatttc tattgttaaa gatattcaga atgtttcatt cctacactag tatttattgc
     3961 ccaaggggtt tgtgagggtt atattggtgt catagcacaa tgccaccact gaaccccccg
     4021 tccaaatttt attctggggg cgtcacctga aaccttgttt tcgagcacct cacatacacc
     4081 ttactgttca caactcagca gttattctat tagctaaacg aaggagaatg aagaagcagg
     4141 cgaagattca ggagagttca ctgcccgctc cttgatcttc agccactgcc cttgtgacta
     4201 aaatggttca ctaccctcgt ggaatcctga ccccatgtaa ataaaaccgt gacagctcat
     4261 ggggtgggag ataacaaggg ggccttataa acactattgc taatgccctc ttgagggtcc
     4321 gcttatcggt agctacacag gcccctctga ttgacgttgg tgtagcctcc cgtagtcttc
     4381 ctgggcccct gggaggtaca tgtcccccag cattggtgta agagcttcag ccaagagtta
     4441 cacataaagg caatgttgtg ttgcagtcca cagactgcaa agtctgctcc aggatgaaag
     4501 ccactcagtg ttggcaaatg tgcacatcca tttataagga tgtcaactac agtcagagaa
     4561 cccctttgtg tttggtcccc ccccgtgtca catgtggaac agggcccagt tggcaagttg
     4621 taccaaccaa ctgaagggat tacatgcact gcccgtgacc aatacaaaac aaaagcgctc
     4681 ctcgtaccag cgaagaaggg gcagagatgc cgttagtcag gtttagttcg tccggcggcg
     4741 ggggatcctc tagagtcgac ctgcagccca agcttggcac tggcgccaga aatccgcgcg
     4801 gtggtttttg ggggtcgggg gtgtttggca gccacagacg cccggtgttc gtgtcgcgcc
     4861 agtacatgcg gtccatgccc aggccatcca aaaaccatgg gtctgtctgc tcagtccagt
     4921 cgtggacctg accccacgca acgcccaaaa gaataacccc cacgaaccat aaaccattcc
     4981 ccatggggga ccccgtccct aacccacggg gcccgtggct atggcgggct tgccgccccg
     5041 acgttggctg cgagccctgg gccttcaccc gaacttgggg gttggggtgg ggaaaaggaa
     5101 gaaacgcggg cgtattggcc ccaatgggct ctcggtgggg tatcgacaga gtgccagccc
     5161 tgggaccgaa ccccgcgttt atgaacaaac gacccaacac ccgtgcgttt tattctgtct
     5221 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     5281 cctcccccat ctcccgatcc ggggcgtcgg tttccactat cggcgagtac ttctacacag
     5341 ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct
     5401 ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg
     5461 tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc
     5521 ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa
     5581 gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc
     5641 gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag
     5701 ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc
     5761 tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga
     5821 tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt
     5881 ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca
     5941 tccatggcct ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc
     6001 aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca
     6061 atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga
     6121 tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca
     6181 tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg
     6241 tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata
     6301 tctcattgcc cccggacgag ggatctgcgg cacgctgttg acgctgttaa gcgggtcgct
     6361 gcagggtcgc tcggtgttcg aggccacacg cgtcacctta atatgcgaag tggacctggg
     6421 accgcgccgc cccgactgca tctgcgtgtt cgaattcgcc aatgacaaga cgctgggcgg
     6481 ggtttgtgtc atcatagaac taaagacatg caaatatatt tcttccgggg acaccgccag
     6541 caaacgcgag caacgggcca cggggatgaa gcagggcagc gctctgggtc attttcggcg
     6601 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
     6661 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
     6721 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgaag
     6781 ccaacacggg aacagaaaac ggataccaag gcgaagaatc tttatttaac aaagcgtact
     6841 acggcggcgg cacgaacttc ttccgtaaag aaagccagaa gcttcagcag agcgctaaaa
     6901 aacgcgatgc tgagttagcg aacggcgccc tcggtatcat agagttaaat aatgattaca
     6961 cattgaaaaa agtaatgaag ccgctgatca cttcaaacac ggtaactgat gaaatcgagc
     7021 gcgcgaatgt tttcaaaatg aacggcaaat ggtacttgtt cactgattca cgcggttcaa
     7081 aaatgacgat cgatggtatt aactcaaacg atatttacat gcttggttat gtatcaaact
     7141 ctttaaccgg cccttacaag ccgctgaaca aaacagggct tgtgctgcaa atgggtcttg
     7201 atccaaacga tgtgacattc acttactctc acttcgcagt gccgcaagcc aaaggcaaca
     7261 atgtggttat cacaagctac atgacaaaca gaggcttctt cgaggataaa aaggcaacat
     7321 ttgcgccaag cttcttaatg aacatcaaag gcaataaaac atccgttgtc aaaaacagca
     7381 tcctggagca aggacagctg acagtcaact aataacagca aaaagaaaat gccgatactt
     7441 cattggcatt ttcttttatt tctcaacaag atggtgaatt gactagtggg tagatccaca
     7501 ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca
     7561 ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt
     7621 gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga
     7681 cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca
     7741 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac
     7801 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc
     7861 aaacatgaga attgatccgg aacccttaat ataacttcgt ataatgtatg ctatacgaag
     7921 ttattaggtc cctcgactat agggtcaccg tcgacagcga cacacttgca tcggatgcag
     7981 cccggttcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc
     8041 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga
     8101 cagctaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8161 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8221 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8281 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8341 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8401 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8461 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8521 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8581 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8641 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8701 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8761 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8821 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8881 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8941 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9001 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9061 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9121 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttatgtcgcg actagtcaat
     9181 tcgggaggat cgaaacggca gatcgcaaaa aacagtacat acagaaggag acatgaacat
     9241 gaacatcaaa aaaattgtaa aacaagccac agttctgact tttacgactg cacttctggc
     9301 aggaggagcg actcaagcct tcgcgaaaga aaataaccaa aaagcataca aagaaacgta
     9361 cggcgtctct catattacac gccatgatat gctgcagatc cctaaacagc agcaaaacga
     9421 aaaataccaa gtgcctcaat tcgatcaatc aacgattaaa aatattgagt ctgcaaaagg
     9481 acttgatgtg tgggacagct ggccgctgca aaacgctgac ggaacagtag ctgaatacaa
     9541 cggctatcac gttgtgtttg ctcttgcggg aagcccgaaa gacgctgatg acacatcaat
     9601 ctacatgttt tatcaaaagg tcggcgacaa ctcaatcgac agctggaaaa acgcgggccg
     9661 tgtctttaaa gacagcgata agttcgacgc caacgatccg atcctgaaag atcagacgca
     9721 agaatggtcc ggttctgcaa cctttacatc tgacggaaaa atccgtttat tctacactga
     9781 ctattccggt aaacattacg gcaaacaaag cctgacaaca gcgcaggtaa atgtgtcaaa
     9841 atctgatgac acactcaaaa tcaacggagt ggaagatcac aaaacgattt ttgacggaga
     9901 cggaaaaaca tatcagaacg ttcagcagtt tatcgatgaa ggcaattata catccggcga
     9961 caaccatacg ctgagagacc ctcactacgt tgaagcttac aggtcgagga gggtctgaag
    10021 aggagtttac gtccagccaa gctagcttgg ctgcaggtcg tcgaaattct accgggtagg
    10081 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
    10141 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
    10201 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
    10261 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
    10321 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
    10381 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
    10441 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
    10501 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
    10561 tccgggcctt tcgacctgca gcctgttgac aattaatcat cggcatagta tatcggcata
    10621 gtataatacg acaaggtgag gaactaaacc atgggatcgg ccattgaaca agatggattg
    10681 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
    10741 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
    10801 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
    10861 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
    10921 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
    10981 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
    11041 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
    11101 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
    11161 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc
    11221 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
    11281 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
    11341 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
    11401 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaggggat
    11461 caattctcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
    11521 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
    11581 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
    11641 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
    11701 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgac tagagcttgc
    11761 ggaaccctgt tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    11821 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    11881 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
    11941 gaggcccttt cgtcttcaag aattctcatg tttgacagct tatcatcgat a
//
