LOCUS       sequence-205774-        2814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC4
FEATURES             Location/Qualifiers
     source          1..2814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     71..88
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(683..700)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(769..787)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        855..959
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1181..1972
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2143..2731
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2632..2651
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
       61 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
      121 tctccccgcg cgttggccga ttcattaatc actctgtggt ctcaaatgtc atcgccaagc
      181 aaacgcagag aaatggatat gatgaagctg atgatgagcg attataaagt ggaaacgatc
      241 aacgatggca tgcaagaatt ctatgttgaa ttcaatggtc ccaaagacag tctctatcaa
      301 ggaggtgtgt ggaagataag agttgagctt ccagatgctt atccttataa atctccatct
      361 gttggtttca ttactaaaat ttatcatcct aatgttgatg aactgtcggg ttctgtttgt
      421 ttagatgtga ttaaccaaac ttggagtcct atgttcgacc ttgtgaatgt gtttgagaca
      481 tttcttcctc agcttcttct gtatccaaac ccatcagatc cattgaatgg agaagctgct
      541 gcgttaatga tgcgtgatcg tcctgcttat gagcaacgag ttaaagaata ctgtgagaag
      601 tatgcaaagc caggggaagg ttcagaagat aagtctagcg atgaagaact aagtgaagag
      661 gaatacggct cagataatga ggatgatgat gatgatgatg ttgcaattgc tggcaaacca
      721 gatccttgag ctttgagacc acgaagtggc tcttcagtgg acgaaagggc ctcgtgatac
      781 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      841 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      901 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      961 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg
     1021 cggttttcat ggcttgttat gactgttttt ttttggggta cagtctatgc ctcgggcatc
     1081 caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt
     1141 tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag cggtgatcgc
     1201 cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac
     1261 gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc cacacagcga
     1321 tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc gagctttgat
     1381 caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc gcgctgtaga
     1441 agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta agcgcgaact
     1501 gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc cagccacgat
     1561 cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg ccttggtagg
     1621 tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg aggcgctaaa
     1681 tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc gaaatgtagt
     1741 gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt
     1801 cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca tacttgaagc
     1861 tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga
     1921 agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat aactgtcaga
     1981 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     2041 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     2101 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2161 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2221 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2281 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2341 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2401 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2461 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2521 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2581 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2641 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2701 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2761 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctga
//
