LOCUS       sequence-205776-       11521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation S585P conferring gain of
            function phenotype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTS585P-myc
FEATURES             Location/Qualifiers
     source          1..11521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(711..729)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        797..901
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(902..970,971..1762)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1120..1139)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1933..2521
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2422..2441
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2952..3097
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3140..4177
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4175..4194)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4219..4267
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4921..5300
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5301..5504
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5455..5475
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5501..5525
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5553..5562
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6228..6257
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7546..7680
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7570..7589)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7624..7643
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7823..7845)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8405..10194
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 tcctgctcct gttccaccgt gggtcccttt gcagccaatg caacttggac gtttttgggg
       61 tctccggaca ccatctctat gtcttggccc tgatcctgag ccgcccgggg ctcctggtct
      121 tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac cccctcttct
      181 ttgaggtcca ctgccgccgg agccttctgg tccagatgtg tctcccttct ctcctaggcc
      241 atttccaggt cctgtacctg gcccctcgtc agacatgatt cacactaaaa gagatcaata
      301 gacatcttta ttagacgacg ctcagtgaat acagggagtg cagactcctg ccccctccaa
      361 cagccccccc accctcatcc ccttcatggt cgctgtcaga cagatccagg tctgaaaatt
      421 ccccatcctc cgaaccatcc tcgtcctcat caccaattac tcgcagcccg gaaaactccc
      481 gctgaacatc ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc
      541 cctttttgct ggacggtagg gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt
      601 caccagacag agatgctact ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc
      661 agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat
      721 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      781 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      841 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      901 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      961 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     1021 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     1081 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     1141 ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     1201 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     1261 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     1321 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     1381 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     1441 gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     1501 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     1561 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1621 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1681 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1741 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1801 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1861 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1921 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1981 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2041 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2101 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2161 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2221 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2281 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2341 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2401 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2461 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2521 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt gaagctgtcc
     2581 ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc
     2641 gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag
     2701 caagacgtag cccagcgcgt cggccccgag atgcgccgcg tgcggctgct ggagatggcg
     2761 gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat
     2821 tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccc tgcttcatcc
     2881 ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat ttgcatgtct
     2941 ttagttctat gatgacacaa accccgccca gcgtcttgtc attggcgaat tcgaacacgc
     3001 agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg acgcgtgtgg
     3061 cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga
     3121 tcccgggggg caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt
     3181 tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc
     3241 tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc
     3301 cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat
     3361 tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg
     3421 tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc
     3481 ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg
     3541 cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat
     3601 tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt
     3661 cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct
     3721 cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt
     3781 cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt
     3841 ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc
     3901 ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact
     3961 ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga
     4021 cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc
     4081 ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag
     4141 cactcgtccg gatcgggaga tgggggaggc taactgaaac acggaaggag acaataccgg
     4201 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg
     4261 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     4321 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     4381 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agccactggc
     4441 cccgtgggtt agggacgggg tcccccatgg ggaatggttt atggttcgtg ggggttatta
     4501 ttttgggcgt tgcgtggggt caggtccacg actggactga gcagacagac ccatggtttt
     4561 tggatggcct gggcatggac cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc
     4621 tgccaaacac ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc caagctagtc
     4681 gaccaattct catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc
     4741 tgcaggcgca gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag
     4801 ccatattagt cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt
     4861 tgtatctata tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt
     4921 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4981 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     5041 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     5101 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     5161 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     5221 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
     5281 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
     5341 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     5401 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
     5461 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     5521 agatctctag aagctgggta ccagctgcta gcgccaccat ggagaccacc cccctgaaca
     5581 gtcaaaagca actctctgcc tgtgaggatg gagaggactg tcaggagaat ggcgttcttc
     5641 aaaaggttgt acccacccct ggagataagg tggaaagcgg ccagatttcc aacggctaca
     5701 gcgccgtgcc atcacctgga gccggggatg atacacggca cagcattccc gcaaccacca
     5761 cgacactggt tgcagagctt caccagggcg agagggagac gtggggcaaa aaggtagatt
     5821 ttctgctttc cgtgatcggt tacgcggtcg atctggggaa cgtgtggagg ttcccttaca
     5881 tttgttacca aaacggaggg ggtgccttct tgctgcccta cacaattatg gcgatttttg
     5941 ggggtatccc gctcttctac atggaactgg ccttgggtca gtaccaccgg aacggctgca
     6001 ttagcatttg gcgcaagata tgccccattt tcaagggaat cggatacgca atctgtataa
     6061 tcgcgttcta tattgcatca tattacaaca ccatcatggc ctgggccctt tattacttga
     6121 tcagcagttt caccgaccaa ttgccgtgga cgagctgtaa gaactcatgg aataccggga
     6181 actgtaccaa ctacttctct gaggacagct caggaggtag cgggggtgaa cagaagctga
     6241 ttagcgagga ggacttgggc agtccaggcg gaagcggcaa catcacttgg acactccata
     6301 gtacgagccc cgcagaggag ttttacacca ggcacgtact gcagattcat aggagcaagg
     6361 gtctgcaaga cctgggcggt attagctggc agctcgcatt gtgcataatg ttgatcttta
     6421 cggtgatcta tttctctatc tggaagggag taaagacatc cggcaaggtg gtgtgggtga
     6481 cggcgacctt cccctacatt atactcagcg tgttgttggt gaggggggca accctgcctg
     6541 gggcctggcg aggcgtgctg ttctatctca agccaaactg gcagaagctt cttgaaaccg
     6601 gagtgtggat cgacgcggcc gcgcaaatct tcttttccct cggtcccggc ttcggggtcc
     6661 ttctggcatt cgcaagctac aacaagttca acaataactg ctaccaggac gccctcgtga
     6721 caagtgtagt gaactgcatg acctctttcg tgagcgggtt tgtgatcttc acagtactcg
     6781 gatatatggc tgagatgagg aacgaagatg ttagtgaggt tgccaaagac gctggcccga
     6841 gcctgttgtt catcacatac gccgaggcta tcgcgaacat gcccgcatct actttcttcg
     6901 ccatcatctt tttcctgatg ctgataaccc ttgggctgga tagcaccttc gccggactgg
     6961 agggtgtcat cactgccgtt ctcgacgagt tcccccatgt gtgggccaag cgacgggaaa
     7021 ggttcgtgct ggccgtcgta atcacatgct tcttcggctc actggtgacc cttacctttg
     7081 gcggtgcgta cgtggtcaag ctgctcgaag agtacgccac tggaccggcg gttctgaccg
     7141 tggcattgat cgaggccgtg gccgtaagct ggttttacgg catcacgcag ttctgtaggg
     7201 acgtgaaaga gatgctgggc ttttctcccg gttggttttg gaggatttgt tgggtagcaa
     7261 ttagccccct gtttctcctt ttcattattt gctcttttct gatgtctcca ccgcagctcc
     7321 gactgtttca gtacaactac ccctactggt caattatcct gggctattgt atcggcacct
     7381 cacctttcat ctgcatcccc acatacatcg catacagact gataattacg cccggtactt
     7441 ttaaggagcg aatcattaaa agcataaccc ccgaaacccc caccgagatc ccttgtggcg
     7501 atataagact caatgcggtc taatagctcg aggccggcaa ggccggatcc agacatgata
     7561 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     7621 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     7681 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     7741 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatccggc tgcctcgcgc
     7801 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     7861 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     7921 ggtgtcgggg cgcagccatg aggtcgactc tagaggatcg atccccgccc cggacgaact
     7981 aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa tcccttcagt
     8041 tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg gggaccaaac
     8101 acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac atttgccaac
     8161 actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac acaacattgc
     8221 ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat gtacctccca
     8281 ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc tgtgtagcta
     8341 ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc ccttgttaac
     8401 cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac taaccctaat
     8461 tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt agtaaaaggg
     8521 tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt tacatggggt
     8581 caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg aagatcaagg
     8641 agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc gtttagctaa
     8701 tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa aacaaggttt
     8761 caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat tgtgctatga
     8821 caccaatata accctcacaa accccttggg caataaatac tagtgtagga atgaaacatt
     8881 ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag actggatgtc
     8941 catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata tgatactggg
     9001 gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt tacactctat
     9061 ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg ttgtctctaa
     9121 cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa gacaagtggc
     9181 cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac gccgccctgc
     9241 ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc gctaaccact
     9301 gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac ctgcataagt
     9361 aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa ttacacacac
     9421 ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt cgctgagagc
     9481 acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga tagcatatgc
     9541 tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     9601 tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg tagcataggc
     9661 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     9721 tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg tagtatatgc
     9781 tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc atatgctatc
     9841 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc ataggctatc
     9901 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     9961 ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
    10021 ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc atatgctatc
    10081 ctcatgcata tacagtcagc atatgatacc cagtagtaga gtgggagtgc tatcctttgc
    10141 atatgccgcc acctcccaag ggggcgtgaa ttttcgctgc ttgtcctttt cctgctggtt
    10201 gctcccattc ttaggtgaat ttaaggaggc caggctaaag ccgtcgcatg tctgattgct
    10261 caccaggtaa atgtcgctaa tgttttccaa cgcgagaagg tgttgagcgc ggagctgagt
    10321 gacgtgacaa catgggtatg cccaattgcc ccatgttggg aggacgaaaa tggtgacaag
    10381 acagatggcc agaaatacac caacagcacg catgatgtct actggggatt tattctttag
    10441 tgcgggggaa tacacggctt ttaatacgat tgagggcgtc tcctaacaag ttacatcact
    10501 cctgcccttc ctcaccctca tctccatcac ctccttcatc tccgtcatct ccgtcatcac
    10561 cctccgcggc agccccttcc accataggtg gaaaccaggg aggcaaatct actccatcgt
    10621 caaagctgca cacagtcacc ctgatattgc aggtaggagc gggctttgtc ataacaaggt
    10681 ccttaatcgc atccttcaaa acctcagcaa atatatgagt ttgtaaaaag accatgaaat
    10741 aacagacaat ggactccctt agcgggccag gttgtgggcc gggtccaggg gccattccaa
    10801 aggggagacg actcaatggt gtaagacgac attgtggaat agcaagggca gttcctcgcc
    10861 ttaggttgta aagggaggtc ttactacctc catatacgaa cacaccggcg acccaagttc
    10921 cttcgtcggt agtcctttct acgtgactcc tagccaggag agctcttaaa ccttctgcaa
    10981 tgttctcaaa tttcgggttg gaacctcctt gaccacgatg ctttccaaac caccctcctt
    11041 ttttgcgcct gcctccatca ccctgacccc ggggtccagt gcttgggcct tctcctgggt
    11101 catctgcggg gccctgctct atcgctcccg ggggcacgtc aggctcacca tctgggccac
    11161 cttcttggtg gtattcaaaa taatcggctt cccctacagg gtggaaaaat ggccttctac
    11221 ctggaggggg cctgcgcggt ggagacccgg atgatgatga ctgactactg ggactcctgg
    11281 gcctcttttc tccacgtcca cgacctctcc ccctggctct ttcacgactt ccccccctgg
    11341 ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg cctccactac
    11401 ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct gctcctgccc
    11461 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg
    11521 c
//
