LOCUS       sequence-205777-       12142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized mutant hSERT with 
            internal MYC tag inserted in ECL2, mutation S585P conferring gain of
            function phenotype.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERTS585P-myc
FEATURES             Location/Qualifiers
     source          1..12142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    14..148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(38..57)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     92..111
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(291..313)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      873..2662
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2964..4886)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGAGGAGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRGG
                     SGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPPP
                     GRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGRR
                     KKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSLY
                     NLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLKD
                     AIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDEG
                     EEGQE"
     primer_bind     complement(5321..5339)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5407..5511
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5512..5580,5581..6372)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5730..5749)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6543..7131
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7032..7051
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7562..7707
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7750..8787
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(8785..8804)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8829..8877
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        9531..9910
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9911..10114
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10065..10085
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10111..10135
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      10163..10172
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10838..10867
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gccggcaagg ccggatccag acatgataag atacattgat gagtttggac aaaccacaac
       61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      121 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      181 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
      241 ggctgattat gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta
      421 gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc
      481 gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt
      541 ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc
      601 ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg
      661 cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac
      721 accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca
      781 acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca
      841 atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt
      901 agtatatact atccagacta accctaattc aatagcatat gttacccaac gggaagcata
      961 tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg
     1021 agctgtcacg gttttattta catggggtca ggattccacg agggtagtga accattttag
     1081 tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct
     1141 gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt
     1201 gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg
     1261 ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca
     1321 ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg
     1381 tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca
     1441 cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag
     1501 acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac
     1561 agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca
     1621 atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc
     1681 acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac
     1741 ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa
     1801 tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc
     1861 tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg
     1921 tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat
     1981 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta
     2041 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     2101 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     2161 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
     2221 tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta
     2281 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct
     2341 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     2401 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     2461 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     2521 aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca
     2581 gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt
     2641 ttcgctgctt gtccttttcc tgctggttgc tcccattctt aggtgaattt aaggaggcca
     2701 ggctaaagcc gtcgcatgtc tgattgctca ccaggtaaat gtcgctaatg ttttccaacg
     2761 cgagaaggtg ttgagcgcgg agctgagtga cgtgacaaca tgggtatgcc caattgcccc
     2821 atgttgggag gacgaaaatg gtgacaagac agatggccag aaatacacca acagcacgca
     2881 tgatgtctac tggggattta ttctttagtg cgggggaata cacggctttt aatacgattg
     2941 agggcgtctc ctaacaagtt acatcactcc tgcccttcct caccctcatc tccatcacct
     3001 ccttcatctc cgtcatctcc gtcatcaccc tccgcggcag ccccttccac cataggtgga
     3061 aaccagggag gcaaatctac tccatcgtca aagctgcaca cagtcaccct gatattgcag
     3121 gtaggagcgg gctttgtcat aacaaggtcc ttaatcgcat ccttcaaaac ctcagcaaat
     3181 atatgagttt gtaaaaagac catgaaataa cagacaatgg actcccttag cgggccaggt
     3241 tgtgggccgg gtccaggggc cattccaaag gggagacgac tcaatggtgt aagacgacat
     3301 tgtggaatag caagggcagt tcctcgcctt aggttgtaaa gggaggtctt actacctcca
     3361 tatacgaaca caccggcgac ccaagttcct tcgtcggtag tcctttctac gtgactccta
     3421 gccaggagag ctcttaaacc ttctgcaatg ttctcaaatt tcgggttgga acctccttga
     3481 ccacgatgct ttccaaacca ccctcctttt ttgcgcctgc ctccatcacc ctgaccccgg
     3541 ggtccagtgc ttgggccttc tcctgggtca tctgcggggc cctgctctat cgctcccggg
     3601 ggcacgtcag gctcaccatc tgggccacct tcttggtggt attcaaaata atcggcttcc
     3661 cctacagggt ggaaaaatgg ccttctacct ggagggggcc tgcgcggtgg agacccggat
     3721 gatgatgact gactactggg actcctgggc ctcttttctc cacgtccacg acctctcccc
     3781 ctggctcttt cacgacttcc ccccctggct ctttcacgtc ctctaccccg gcggcctcca
     3841 ctacctcctc gaccccggcc tccactacct cctcgacccc ggcctccact gcctcctcga
     3901 ccccggcctc cacctcctgc tcctgcccct cctgctcctg cccctcctcc tgctcctgcc
     3961 cctcctgctc ctgcccctcc cgctcctgcc cctcctgctc ctgcccctcc tgcccctcct
     4021 gctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tcctgctcct
     4081 gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc ccctcctgcc
     4141 cctcctgctc ctgcccctcc tgctcctgcc cctcctgctc ctgcccctcc tgctcctgcc
     4201 cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgctcctgc ccctcctgcc
     4261 cctcctgccc ctcctgctcc tgcccctcct cctgctcctg cccctcctgc ccctcctgcc
     4321 cctcctcctg ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc tcctgctcct
     4381 gcccctcctg cccctcctgc ccctcctcct gctcctgccc ctcctgcccc tcctcctgct
     4441 cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctcc tgctcctgcc
     4501 cctcctcctg ctcctgcccc tcctgcccct cctgcccctc ctgcccctcc tcctgctcct
     4561 gcccctcctc ctgctcctgc ccctcctgct cctgcccctc ccgctcctgc tcctgctcct
     4621 gttccaccgt gggtcccttt gcagccaatg caacttggac gtttttgggg tctccggaca
     4681 ccatctctat gtcttggccc tgatcctgag ccgcccgggg ctcctggtct tccgcctcct
     4741 cgtcctcgtc ctcttccccg tcctcgtcca tggttatcac cccctcttct ttgaggtcca
     4801 ctgccgccgg agccttctgg tccagatgtg tctcccttct ctcctaggcc atttccaggt
     4861 cctgtacctg gcccctcgtc agacatgatt cacactaaaa gagatcaata gacatcttta
     4921 ttagacgacg ctcagtgaat acagggagtg cagactcctg ccccctccaa cagccccccc
     4981 accctcatcc ccttcatggt cgctgtcaga cagatccagg tctgaaaatt ccccatcctc
     5041 cgaaccatcc tcgtcctcat caccaattac tcgcagcccg gaaaactccc gctgaacatc
     5101 ctcaagattt gcgtcctgag cctcaagcca ggcctcaaat tcctcgtccc cctttttgct
     5161 ggacggtagg gatggggatt ctcgggaccc ctcctcttcc tcttcaaggt caccagacag
     5221 agatgctact ggggcaacgg aagaaaagct gggtgcggcc tgtgaggatc agcttatcga
     5281 tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt
     5341 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5461 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     5521 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     5581 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     5641 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     5701 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac
     5761 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     5821 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     5881 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     5941 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     6001 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     6061 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     6121 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     6181 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     6241 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     6301 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     6361 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     6421 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     6481 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6541 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6601 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6661 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6721 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6781 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6841 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6901 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6961 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     7021 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     7081 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     7141 aacgcggcct ttttacggtt cctggccttt tgctggcctt gaagctgtcc ctgatggtcg
     7201 tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg
     7261 agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag
     7321 cccagcgcgt cggccccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg
     7381 atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc
     7441 caattcttgg agtggtgaat ccgttagcga ggtgccgccc tgcttcatcc ccgtggcccg
     7501 ttgctcgcgt ttgctggcgg tgtccccgga agaaatatat ttgcatgtct ttagttctat
     7561 gatgacacaa accccgccca gcgtcttgtc attggcgaat tcgaacacgc agatgcagtc
     7621 ggggcggcgc ggtccgaggt ccacttcgca tattaaggtg acgcgtgtgg cctcgaacac
     7681 cgagcgaccc tgcagcgacc cgcttaacag cgtcaacagc gtgccgcaga tcccgggggg
     7741 caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     7801 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     7861 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     7921 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     7981 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     8041 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     8101 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     8161 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     8221 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     8281 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     8341 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     8401 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     8461 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     8521 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     8581 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     8641 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     8701 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     8761 gatcgggaga tgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
     8821 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata
     8881 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
     8941 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
     9001 cagggctcgc agccaacgtc ggggcggcag gccctgccat agccactggc cccgtgggtt
     9061 agggacgggg tcccccatgg ggaatggttt atggttcgtg ggggttatta ttttgggcgt
     9121 tgcgtggggt caggtccacg actggactga gcagacagac ccatggtttt tggatggcct
     9181 gggcatggac cgcatgtact ggcgcgacac gaacaccggg cgtctgtggc tgccaaacac
     9241 ccccgacccc caaaaaccac cgcgcggatt tctggcgtgc caagctagtc gaccaattct
     9301 catgtttgac agcttatcat cgcagatccg ggcaacgttg ttgccattgc tgcaggcgca
     9361 gaactggtag gtatggaaga tctatacatt gaatcaatat tggcaattag ccatattagt
     9421 cattggttat atagcataaa tcaatattgg ctattggcca ttgcatacgt tgtatctata
     9481 tcataatatg tacatttata ttggctcatg tccaatatga ccgccatgtt gacattgatt
     9541 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     9601 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     9661 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     9721 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     9781 tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     9841 ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     9901 tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc
     9961 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
    10021 tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag
    10081 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatctctag
    10141 aagctgggta ccagctgcta gcgccaccat ggagaccacc cccctgaaca gtcaaaagca
    10201 actctctgcc tgtgaggatg gagaggactg tcaggagaat ggcgttcttc aaaaggttgt
    10261 acccacccct ggagataagg tggaaagcgg ccagatttcc aacggctaca gcgccgtgcc
    10321 atcacctgga gccggggatg atacacggca cagcattccc gcaaccacca cgacactggt
    10381 tgcagagctt caccagggcg agagggagac gtggggcaaa aaggtagatt ttctgctttc
    10441 cgtgatcggt tacgcggtcg atctggggaa cgtgtggagg ttcccttaca tttgttacca
    10501 aaacggaggg ggtgccttct tgctgcccta cacaattatg gcgatttttg ggggtatccc
    10561 gctcttctac atggaactgg ccttgggtca gtaccaccgg aacggctgca ttagcatttg
    10621 gcgcaagata tgccccattt tcaagggaat cggatacgca atctgtataa tcgcgttcta
    10681 tattgcatca tattacaaca ccatcatggc ctgggccctt tattacttga tcagcagttt
    10741 caccgaccaa ttgccgtgga cgagctgtaa gaactcatgg aataccggga actgtaccaa
    10801 ctacttctct gaggacagct caggaggtag cgggggtgaa cagaagctga ttagcgagga
    10861 ggacttgggc agtccaggcg gaagcggcaa catcacttgg acactccata gtacgagccc
    10921 cgcagaggag ttttacacca ggcacgtact gcagattcat aggagcaagg gtctgcaaga
    10981 cctgggcggt attagctggc agctcgcatt gtgcataatg ttgatcttta cggtgatcta
    11041 tttctctatc tggaagggag taaagacatc cggcaaggtg gtgtgggtga cggcgacctt
    11101 cccctacatt atactcagcg tgttgttggt gaggggggca accctgcctg gggcctggcg
    11161 aggcgtgctg ttctatctca agccaaactg gcagaagctt cttgaaaccg gagtgtggat
    11221 cgacgcggcc gcgcaaatct tcttttccct cggtcccggc ttcggggtcc ttctggcatt
    11281 cgcaagctac aacaagttca acaataactg ctaccaggac gccctcgtga caagtgtagt
    11341 gaactgcatg acctctttcg tgagcgggtt tgtgatcttc acagtactcg gatatatggc
    11401 tgagatgagg aacgaagatg ttagtgaggt tgccaaagac gctggcccga gcctgttgtt
    11461 catcacatac gccgaggcta tcgcgaacat gcccgcatct actttcttcg ccatcatctt
    11521 tttcctgatg ctgataaccc ttgggctgga tagcaccttc gccggactgg agggtgtcat
    11581 cactgccgtt ctcgacgagt tcccccatgt gtgggccaag cgacgggaaa ggttcgtgct
    11641 ggccgtcgta atcacatgct tcttcggctc actggtgacc cttacctttg gcggtgcgta
    11701 cgtggtcaag ctgctcgaag agtacgccac tggaccggcg gttctgaccg tggcattgat
    11761 cgaggccgtg gccgtaagct ggttttacgg catcacgcag ttctgtaggg acgtgaaaga
    11821 gatgctgggc ttttctcccg gttggttttg gaggatttgt tgggtagcaa ttagccccct
    11881 gtttctcctt ttcattattt gctcttttct gatgtctcca ccgcagctcc gactgtttca
    11941 gtacaactac ccctactggt caattatcct gggctattgt atcggcacct cacctttcat
    12001 ctgcatcccc acatacatcg catacagact gataattacg cccggtactt ttaaggagcg
    12061 aatcattaaa agcataaccc ccgaaacccc caccgagatc ccttgtggcg atataagact
    12121 caatgcggtc taatagctcg ag
//
