LOCUS       sequence-205779-       10941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TREAT-siRNA reporter in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10941)
  AUTHORS   Horvathova I, Voigt F, Kotrys AV, Zhan Y, Artus-Revel CG, Eglinger 
            J, Stadler MB, Giorgetti L, Chao JA
  TITLE     The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in
            Single Cells.
  JOURNAL   Mol Cell. 2017 Nov 2;68(3):615-625.e9. doi: 
            10.1016/j.molcel.2017.09.030. Epub 2017 Oct 19.
  PUBMED    29056324
REFERENCE   2  (bases 1 to 10941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2017.09.030"; journalName: "Mol Cell"; date: 
            "2017-11-2- 2"; volume: "68"; issue: "3"; pages: "615-625.e9"
COMMENT     Sequence Label: pSF4 TetCMV intron Renilla-STOP 24xPP7
            1xfiref...
FEATURES             Location/Qualifiers
     source          1..10941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(12..28)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12..28)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(25..47)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    36..52
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(60..66,67..84,85..90))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    105..126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(243..260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(414..1002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(494..513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1173..1964,1965..2033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1796..1815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2034..2138)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2206..2224
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2263..2285)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2385..2404
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2598..2620
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2612..2629
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2613..2629
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3716..3763)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    3809..3827
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3845..3863
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3881..3899
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3917..3935
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3953..3971
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3989..4007
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4025..4043
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     4088..4112
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4189..4321
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     4304..4323
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        4366..4384
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4388..4397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4394..5329
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    8734..8868
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8812..8831
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(8976..9009)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(9062..9078)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9094..9116)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9527..9884
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             9947..10540
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
       61 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
      241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      601 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      841 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2161 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2221 aggccctttc gtcatcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2281 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2341 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2401 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     2461 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     2521 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     2581 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattaattcc
     2641 aagctaagtg aaagactctg tctcaaaaac caacctgaca cctgagccgg agcgttggct
     2701 ctcactacca tagaatattt tttcacaaca cacacacaca cacacacaca cacacacaca
     2761 cacacacaca cacagtcata cacatagaca cagtcacaca cacacacatg cacacacaca
     2821 cacacacact cacacacaca catacataca cacattcaca cacacccata cacacatttt
     2881 cacagtcaca cacacaaaca caatcacaca cacacacatc aatcaatcaa tcaatcaata
     2941 taccaaaaat gatagctcat acctgtaatc ttagatgtgg gaaggctaaa acagcaggct
     3001 acccatgact tcaaggtcac cctgggttac agagtagcta atagtctagc ttgaactata
     3061 gtgtaggatc tttaaaaaaa aattaaccaa ccaaccaaca aacaaacaaa caaacaacaa
     3121 caacaacaaa tggaagacag aggacaaaaa aaaataaaag aaaaagtaga aagccaatgg
     3181 agatactatc ttaaaattag aaatatttat gacacattct gaatactcca tgtttatttc
     3241 aaggaagcca atgtaacttt aagtactatg gccatttaat gtaaatactt aagaaaaaaa
     3301 accaaattaa ttttgataca tgctgcatgt gtgaaagacc cccgctgacg ggtagtcaat
     3361 cactcagagg agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag
     3421 ggtttattgg atacacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag
     3481 tgaggggttg tgggctcgag ctcggggagc agaagcgcgc gaacagaagt cgagctagct
     3541 tgccaaacct acaggtgggg tctttcattc cccccttttt ctggagacta aataaaatct
     3601 tttattttat ctatggctcg tactctatag gcttcagctg gtgatattgt tgagtcaaaa
     3661 ctagagcctg gaccactgat atcctgtctt taacaaattg gactaatccg gatctgaagt
     3721 tcctatactt tctagagaat aggaacttcg gaataggaac ttcgcggcag atcttttgcg
     3781 gccgctttgg gccggccgaa ttctttactc cctatcagtg atagagaacg tatgaagagt
     3841 ttactcccta tcagtgatag agaacgtatg cagactttac tccctatcag tgatagagaa
     3901 cgtataagga gtttactccc tatcagtgat agagaacgta tgaccagttt actccctatc
     3961 agtgatagag aacgtatcta cagtttactc cctatcagtg atagagaacg tatatccagt
     4021 ttactcccta tcagtgatag agaacgtata agctttaggc gtgtacggtg ggcgcctata
     4081 aaagcagagc tcgtttagtg aaccgtcaga tcgcctggag caattccaca acacttttgt
     4141 cttataccaa ctttccgtac cacttcctac cctcgtaaaa ccggtcaggt aagtatcaag
     4201 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
     4261 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
     4321 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
     4381 taggctagcc accatggctt ccaaggtgta cgaccccgag caacgcaaac gcatgatcac
     4441 tgggcctcag tggtgggctc gctgcaagca aatgaacgtg ctggactcct tcatcaacta
     4501 ctatgattcc gagaagcacg ccgagaacgc cgtgattttt ctgcatggta acgctgcctc
     4561 cagctacctg tggaggcacg tcgtgcctca catcgagccc gtggctagat gcatcatccc
     4621 tgatctgatc ggaatgggta agtccggcaa gagcgggaat ggctcatatc gcctcctgga
     4681 tcactacaag tacctcaccg cttggttcga gctgctgaac cttccaaaga aaatcatctt
     4741 tgtgggccac gactgggggg cttgtctggc ctttcactac tcctacgagc accaagacaa
     4801 gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg atcgagtcct gggacgagtg
     4861 gcctgacatc gaggaggata tcgccctgat caagagcgaa gagggcgaga aaatggtgct
     4921 tgagaataac ttcttcgtcg agaccatgct cccaagcaag atcatgcgga aactggagcc
     4981 tgaggagttc gctgcctacc tggagccatt caaggagaag ggcgaggtta gacggcctac
     5041 cctctcctgg cctcgcgaga tccctctcgt taagggaggc aagcccgacg tcgtccagat
     5101 tgtccgcaac tacaacgcct accttcgggc cagcgacgat ctgcctaaga tgttcatcga
     5161 gtccgaccct gggttctttt ccaacgctat tgtcgaggga gctaagaagt tccctaacac
     5221 cgagttcgtg aaggtgaagg gcctccactt cagccaggag gacgctccag atgaaatggg
     5281 taagtacatc aagagcttcg tggagcgcgt gctgaagaac gagcagtaag tcgacagatc
     5341 ctacctgcaa agcaccagag gatatggcct cggtgcctca gcatcttcag atgtgctgtc
     5401 actgtccaat cttcaagcca gcagagcata tgggctcgct ggctccactg tcattacagt
     5461 ggacctgatc ttctccaatc aaaggagcag aggatatggc ctcgctccct ccgactacat
     5521 caggctggaa gtgacatcca tcattcatcc cagcagagca tatgggctcg ctgggaccac
     5581 agcatgcctc gaagtgctga gagactatca tcaaaccgac agaggatatg gcctcgtcgg
     5641 ctcctactcc ctcctggtgg aatacttctc cactcttcaa cccagcagag catatgggct
     5701 cgctgggtcc atcagagata caagatctct cgacagatcc tacctgcaaa gcaccagagg
     5761 atatggcctc ggtgcctcag catcttcaga tgtgctgtca ctgtccaatc ttcaagccag
     5821 cagagcatat gggctcgctg gctccactgt cattacagtg gacctgatct tctccaatca
     5881 aaggagcaga ggatatggcc tcgctccctc cgactacatc aggctggaag tgacatccat
     5941 cattcatccc agcagagcat atgggctcgc tgggaccaca gcatgcctcg aagtgctgag
     6001 agactatcat caaaccgaca gaggatatgg cctcgtcggc tcctactccc tcctggtgga
     6061 atacttctcc actcttcaac ccagcagagc atatgggctc gctgggtcca tcagagatac
     6121 aagatctctc gacagatcct acctgcaaag caccagagga tatggcctcg gtgcctcagc
     6181 atcttcagat gtgctgtcac tgtccaatct tcaagccagc agagcatatg ggctcgctgg
     6241 ctccactgtc attacagtgg acctgatctt ctccaatcaa aggagcagag gatatggcct
     6301 cgctccctcc gactacatca ggctggaagt gacatccatc attcatccca gcagagcata
     6361 tgggctcgct gggaccacag catgcctcga agtgctgaga gactatcatc aaaccgacag
     6421 aggatatggc ctcgtcggct cctactccct cctggtggaa tacttctcca ctcttcaacc
     6481 cagcagagca tatgggctcg ctgggtccat cagagataca agatctctcg acagatccta
     6541 cctgcaaagc accagaggat atggcctcgg tgcctcagca tcttcagatg tgctgtcact
     6601 gtccaatctt caagccagca gagcatatgg gctcgctggc tccactgtca ttacagtgga
     6661 cctgatcttc tccaatcaaa ggagcagagg atatggcctc gctccctccg actacatcag
     6721 gctggaagtg acatccatca ttcatcccag cagagcatat gggctcgctg ggaccacagc
     6781 atgcctcgaa gtgctgagag actatcatca aaccgacaga ggatatggcc tcgtcggctc
     6841 ctactccctc ctggtggaat acttctccac tcttcaaccc agcagagcat atgggctcgc
     6901 tgggtccatc agagatacaa gatctctcga gccgcggtgc gtaagtctgt taacagaatg
     6961 ctgcatacgc tgagtacttc gaaatgtcat cctctgtctg acactgaacg taatccagac
     7021 gcgtaagtca ggccggaaaa ttcccgccac cggaagttga gtagacggtg ctgcctgcga
     7081 ctcaacccca ggaggactgg gtgaacaaag ctgcgaagtg atccatgtaa gccctcagaa
     7141 ccgtctcgga aagaggaccc cacatgttgt agcttcaagg cccaatgtca gaccacgcca
     7201 tggcgtgcca ctctgcggag agtgcagtct gcgacagtgc cccaggagga ctgggtgagg
     7261 atcctaccta caaacggcac gagcatcagc cgtgccgccc agcatcttca tcactgtcca
     7321 atcttcaagc gtgcacgatc acggcacgct ccactgtcat tacagtctta tccaatcaaa
     7381 ctccagcagc atcagcgctg gagctccgac tacatcagga catccatcat tcaacgctcg
     7441 acgatcacgc gagcgtccac agcatgcctc agacactatc atcaaacggc tgcacgatca
     7501 cggcagccgc tcctactgcc tctacgttat cgactcttct gcgacgagca tcagccgtcg
     7561 caacatcgga cataccagat cctacctaca aacggcacga gcatcagccg tgccgcccag
     7621 catcttcatc actgtccaat cttcaagcgt gcacgatcac ggcacgctcc actgtcatta
     7681 cagtcttatc caatcaaact ccagcagcat cagcgctgga gctccgacta catcaggaca
     7741 tccatcattc aacgctcgac gatcacgcga gcgtccacag catgcctcag acactatcat
     7801 caaacggctg cacgatcacg gcagccgctc ctactgcctc tacgttatcg actcttctgc
     7861 gacgagcatc agccgtcgca acatcggaca taccagatcc tacctacaaa cggcacgagc
     7921 atcagccgtg ccgcccagca tcttcatcac tgtccaatct tcaagcgtgc acgatcacgg
     7981 cacgctccac tgtcattaca gtcttatcca atcaaactcc agcagcatca gcgctggagc
     8041 tccgactaca tcaggacatc catcattcaa cgctcgacga tcacgcgagc gtccacagca
     8101 tgcctcagac actatcatca aacggctgca cgatcacggc agccgctcct actgcctcta
     8161 cgttatcgac tcttctgcga cgagcatcag ccgtcgcaac atcggacata ccagatccta
     8221 cctacaaacg gcacgagcat cagccgtgcc gcccagcatc ttcatcactg tccaatcttc
     8281 aagcgtgcac gatcacggca cgctccactg tcattacagt cttatccaat caaactccag
     8341 cagcatcagc gctggagctc cgactacatc aggacatcca tcattcaacg ctcgacgatc
     8401 acgcgagcgt ccacagcatg cctcagacac tatcatcaaa cggctgcacg atcacggcag
     8461 ccgctcctac tgcctctacg ttatcgactc ttctgcgacg agcatcagcc gtcgcaacat
     8521 cggacatacc agatcttcaa tcgatttcgc gcgggatcca gaccacctcc cctgcgagct
     8581 aagctggaca gccaatgacg ggtaagagag tgacattttt cactaaccta agacaggagg
     8641 gccgtcagag ctactgccta atccaaagac gggtaaaagt gataaaaatg tatcactcca
     8701 acctaagaca ggcgcagctt ccgagggatt tgagatccag acatgataag atacattgat
     8761 gagtttggac aaaccaaaac tagaatgcag tgaaaaaaat gccttatttg tgaaatttgt
     8821 gatgctattg ccttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     8881 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     8941 aacctctaca aatgtggtat ggctgattat gatctgaagt tcctatacta tttgaagaat
     9001 aggaacttcg gaataggaac ttcggatctc atatggccat atgggggcgc ctagagaagg
     9061 agtgagggct ggataaaggg aggatcgagg cggggtcgaa cgaggaggtt caagggggag
     9121 agacggggcg gatggaggaa gaggaggcgg aggcttaggg tgtacaaagg gcttgaccca
     9181 gggagggggg tcaaaagcca aggcttccca ggtcacgatg taccccacct ggtctgggtg
     9241 tccatgcggg ccaggtgaaa agaccttgat cttaacctgg gtgatgaggt ctcggttaaa
     9301 ggtgccgtct cgcggccatc cgacgttaaa ggttggccat tctgcagagc agaaggtaac
     9361 ccaacgtctc ttcttgacat ctaccgactg gttgtgagcg atccgctcga catctttcca
     9421 gtgacctaag gtcaaactta agggagtggt aacaggctag cccgggccca tattttcaga
     9481 caaatacaga aacacagtca gacagagaca acacagaacg atgctgcagc agacaagacg
     9541 cgcggcgcgg cttcggtccc aaaccgaaag caaaaattca gacggaggcg ggaactgttt
     9601 taggttctcg tctcctacca gaaccacata tccctcctct aaggggggtg caccaaagag
     9661 tcctaaacga tcgggatttt aggactcagg tcgggccaca aaaacggccc ccgaagtccc
     9721 tgggacgtct cccagggttg cggccgggtg ttccgaactc gtcagttcca ccacgggtcc
     9781 gccagataca gagctagtta gctaactagt accgacgcag gcgcataaaa tcagtcatag
     9841 acactagaca atcggacaga cacagataag ttgctggcca gcttacctcc cggtggtggg
     9901 tcggtggtcc ctgggcaggg gtctcccgat cccggacgag cccccaaatg aaagaccccc
     9961 gctgacgggt agtcaatcac tcagaggaga ccctcccaag gaacagcgag accacaagtc
    10021 ggatgcaact gcaagagggt ttattggata cacgggtacc cgggcgactc agtcaatcgg
    10081 aggactggcg ccccgagtga ggggttgtgg gctcttttat tgagctcggg gagcataagc
    10141 gcgcgaacag aagcgagaag cgaactgatt ggttagttca aataaggcac agggtcattt
    10201 caggtccttg gggcaccctg gaaacatctg atggttctct agaaactgct gagggctgga
    10261 ccgcatctgg ggaccatctg ttcttggccc tgagccgggg caggaactgc ttaccacaga
    10321 tatcctgttt ggcccatatt cagctgttcc atctgttctt ggccctgagc cggggcagga
    10381 actgcttacc acagatatcc tgtttggccc atattcagct gttccatctg ttcctgacct
    10441 tgatctgaac ttttctattc tcagttatgt atttttccat gccttgcaaa atggcgttac
    10501 ttaagctagc ttgccaaacc tacaggtggg gtctttcatt cacatgtata tgtcaaaaat
    10561 aaaaatcaac taattgacta gtaattaata tgactggcat aatgggaaat tgatcctgac
    10621 agatgcaaac tggcttgctc agcagcgcat ttatgttgtc aactgaggaa ggaaacgtta
    10681 atgacagaaa ctctaagtaa tttccacgtt tatctatttt tatttatact agctttggta
    10741 acaggaatat tgcagcattc atgcacattg aaacccttat gaaataaaaa catctgtgca
    10801 tttaaaatgg aattaacatt ttaaatgtta aaaaaagctg gcttagcttc cccccgcccc
    10861 ctagggcata gaacaagtca aatgctttat atatttgagt ttgggatgta ttaggaaact
    10921 cctaagagca aagctagctt g
//
