LOCUS       sequence-205795-       12116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized hNET with internal 
            MYC tag inserted in ECL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12116)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 12116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-NET-myc
FEATURES             Location/Qualifiers
     source          1..12116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    14..148
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(38..57)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     92..111
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(291..313)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      873..2662
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(2968..4893)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(5328..5346)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5414..5518
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5519..5587,5588..6379)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5737..5756)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6550..7138
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7039..7058
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7569..7714
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             7757..8794
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(8792..8811)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8836..8884
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        9538..9917
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9918..10121
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10072..10092
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     10118..10142
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             10791..10820
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gccggcaagg ccggatccag acatgataag atacattgat gagtttggac aaaccacaac
       61 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      121 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      181 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
      241 ggctgattat gatccggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      301 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      361 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgag gtcgactcta
      421 gaggatcgat ccccgccccg gacgaactaa acctgactac gacatctctg ccccttcttc
      481 gcggggcagt gcatgtaatc ccttcagttg gttggtacaa cttgccaact gggccctgtt
      541 ccacatgtga cacggggggg gaccaaacac aaaggggttc tctgactgta gttgacatcc
      601 ttataaatgg atgtgcacat ttgccaacac tgagtggctt tcatcctgga gcagactttg
      661 cagtctgtgg actgcaacac aacattgcct ttatgtgtaa ctcttggctg aagctcttac
      721 accaatgctg ggggacatgt acctcccagg ggcccaggaa gactacggga ggctacacca
      781 acgtcaatca gaggggcctg tgtagctacc gataagcgga ccctcaagag ggcattagca
      841 atagtgttta taaggccccc ttgttaaccc taaacgggta gcatatgctt cccgggtagt
      901 agtatatact atccagacta accctaattc aatagcatat gttacccaac gggaagcata
      961 tgctatcgaa ttagggttag taaaagggtc ctaaggaaca gcgatatctc ccaccccatg
     1021 agctgtcacg gttttattta catggggtca ggattccacg agggtagtga accattttag
     1081 tcacaagggc agtggctgaa gatcaaggag cgggcagtga actctcctga atcttcgcct
     1141 gcttcttcat tctccttcgt ttagctaata gaataactgc tgagttgtga acagtaaggt
     1201 gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc ccagaataaa atttggacgg
     1261 ggggttcagt ggtggcattg tgctatgaca ccaatataac cctcacaaac cccttgggca
     1321 ataaatacta gtgtaggaat gaaacattct gaatatcttt aacaatagaa atccatgggg
     1381 tggggacaag ccgtaaagac tggatgtcca tctcacacga atttatggct atgggcaaca
     1441 cataatccta gtgcaatatg atactggggt tattaagatg tgtcccaggc agggaccaag
     1501 acaggtgaac catgttgtta cactctattt gtaacaaggg gaaagagagt ggacgccgac
     1561 agcagcggac tccactggtt gtctctaaca cccccgaaaa ttaaacgggg ctccacgcca
     1621 atggggccca taaacaaaga caagtggcca ctcttttttt tgaaattgtg gagtgggggc
     1681 acgcgtcagc ccccacacgc cgccctgcgg ttttggactg taaaataagg gtgtaataac
     1741 ttggctgatt gtaaccccgc taaccactgc ggtcaaacca cttgcccaca aaaccactaa
     1801 tggcaccccg gggaatacct gcataagtag gtgggcgggc caagataggg gcgcgattgc
     1861 tgcgatctgg aggacaaatt acacacactt gcgcctgagc gccaagcaca gggttgttgg
     1921 tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa tgttgccatg ggtagcatat
     1981 actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta gcataggcta
     2041 tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta
     2101 tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta
     2161 tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta gcatatgcta
     2221 tcctaataga gattagggta gtatatgcta tcctaattta tatctgggta gcatatacta
     2281 cccaaatatc tggatagcat atgctatcct aatctatatc tgggtagcat atgctatcct
     2341 aatctatatc tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct
     2401 aatctatatc tgggtagtat atgctatcct aatttatatc tgggtagcat aggctatcct
     2461 aatctatatc tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct
     2521 aatctgtatc cgggtagcat atgctatcct catgcatata cagtcagcat atgataccca
     2581 gtagtagagt gggagtgcta tcctttgcat atgccgccac ctcccaaggg ggcgtgaatt
     2641 ttcgctgctt gtccttttcc tgcatgctgg ttgctcccat tcttaggtga atttaaggag
     2701 gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct aatgttttcc
     2761 aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta tgcccaattg
     2821 ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac accaacagca
     2881 cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc ttttaatacg
     2941 attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct catctccatc
     3001 acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt ccaccatagg
     3061 tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca ccctgatatt
     3121 gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca aaacctcagc
     3181 aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc ttagcgggcc
     3241 aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg gtgtaagacg
     3301 acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg tcttactacc
     3361 tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt ctacgtgact
     3421 cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt tggaacctcc
     3481 ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat caccctgacc
     3541 ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct ctatcgctcc
     3601 cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa aataatcggc
     3661 ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg gtggagaccc
     3721 ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc cacgacctct
     3781 ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac cccggcggcc
     3841 tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc cactgcctcc
     3901 tcgaccccgg cctccacctc ctgctcctgc ccctcctgct cctgcccctc ctcctgctcc
     3961 tgcccctcct gcccctcctg ctcctgcccc tcctgcccct cctgctcctg cccctcctgc
     4021 ccctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg cccctcctcc
     4081 tgctcctgcc cctcctgccc ctcctgctcc tgcccctcct gcccctcctg ctcctgcccc
     4141 tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg cccctcctgc
     4201 tcctgcccct cctgcccctc ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc
     4261 tcctgcccct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc ctcctgcccc
     4321 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctcc
     4381 tgctcctgcc cctcctgccc ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc
     4441 tcctgctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctgccc ctcctcctgc
     4501 tcctgcccct cctcctgctc ctgcccctcc tgcccctcct gcccctcctg cccctcctcc
     4561 tgctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcccg ctcctgctcc
     4621 tgctcctgtt ccaccgtggg tccctttgca gccaatgcaa cttggacgtt tttggggtct
     4681 ccggacacca tctctatgtc ttggccctga tcctgagccg cccggggctc ctggtcttcc
     4741 gcctcctcgt cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg
     4801 aggtccactg ccgccggagc cttctggtcc agatgtgtct cccttctctc ctaggccatt
     4861 tccaggtcct gtacctggcc cctcgtcaga catgattcac actaaaagag atcaatagac
     4921 atctttatta gacgacgctc agtgaataca gggagtgcag actcctgccc cctccaacag
     4981 cccccccacc ctcatcccct tcatggtcgc tgtcagacag atccaggtct gaaaattccc
     5041 catcctccga accatcctcg tcctcatcac caattactcg cagcccggaa aactcccgct
     5101 gaacatcctc aagatttgcg tcctgagcct caagccaggc ctcaaattcc tcgtccccct
     5161 ttttgctgga cggtagggat ggggattctc gggacccctc ctcttcctct tcaaggtcac
     5221 cagacagaga tgctactggg gcaacggaag aaaagctggg tgcggcctgt gaggatcagc
     5281 ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg
     5341 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5401 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5461 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5521 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5581 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5641 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     5701 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     5761 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     5821 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     5881 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     5941 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6001 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6061 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6121 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6181 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6241 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6301 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6361 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6421 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6481 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6541 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6601 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6661 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     6721 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     6781 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     6841 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     6901 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     6961 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7021 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7081 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7141 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg
     7201 atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc
     7261 ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa
     7321 gacgtagccc agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga gatggcggac
     7381 gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga
     7441 ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg
     7501 tggcccgttg ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta
     7561 gttctatgat gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga
     7621 tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct
     7681 cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc
     7741 cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     7801 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     7861 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     7921 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     7981 ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc
     8041 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     8101 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     8161 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     8221 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     8281 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     8341 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     8401 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     8461 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     8521 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     8581 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     8641 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     8701 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     8761 tcgtccggat cgggagatgg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
     8821 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt tgggtcgttt
     8881 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
     8941 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
     9001 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc cactggcccc
     9061 gtgggttagg gacggggtcc cccatgggga atggtttatg gttcgtgggg gttattattt
     9121 tgggcgttgc gtggggtcag gtccacgact ggactgagca gacagaccca tggtttttgg
     9181 atggcctggg catggaccgc atgtactggc gcgacacgaa caccgggcgt ctgtggctgc
     9241 caaacacccc cgacccccaa aaaccaccgc gcggatttct ggcgtgccaa gctagtcgac
     9301 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     9361 aggcgcagaa ctggtaggta tggaagatct atacattgaa tcaatattgg caattagcca
     9421 tattagtcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt
     9481 atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac
     9541 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     9601 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     9661 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     9721 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     9781 tgtatcatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     9841 attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag
     9901 tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt
     9961 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
    10021 accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg
    10081 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga
    10141 tctctagaag ctgggtacca gctgctagcg ccaccatgtt gctggctcgc atgaaccccc
    10201 aggtgcagcc cgagaataac ggcgctgata ccggtcccga gcagcccctg agggcaagga
    10261 aaaccgccga gctgttggtg gtaaaggaga ggaatggcgt acagtgcttg ctcgctccca
    10321 gggacggaga cgcccaaccc cgagagacgt ggggcaagaa gatcgacttt ctgttgtctg
    10381 tcgtgggctt cgcggtggat cttgccaatg tgtggaggtt cccttacctg tgttacaaga
    10441 acggcggagg agccttcttg attccttaca ccctgtttct gataatcgcc ggaatgccac
    10501 tgttctacat ggagctggcg ctgggtcagt acaataggga gggagcggcg accgtatgga
    10561 aaatttgccc cttcttcaag ggcgtcggct acgccgtgat cctgattgct ctctacgtcg
    10621 ggttctacta caacgtgatc atcgcctgga gtctgtacta cctgttcagc tcttttacgc
    10681 tcaacttgcc atggacagac tgcggacaca cgtggaactc acccaactgc acggacccca
    10741 aactccttaa cggaagcgtg ctgggcagct ccgggggaag cggaggcggc gagcagaaac
    10801 tgataagcga ggaggacctc ggttcccctg gcggatctag tggtaaccat accaagtata
    10861 gcaagtacaa gtttaccccc gctgcagagt tctacgagag gggcgtcctg catctccatg
    10921 agtctagcgg catacatgac atcggactcc cccaatggca gttgctcctg tgcttgatgg
    10981 tcgtagtgat cgtgctgtac ttttctctct ggaaaggagt caagactagt ggcaaggtgg
    11041 tttggatcac cgccaccctg ccgtattttg tgcttttcgt tctccttgtg catggcgtga
    11101 cccttcccgg tgcatcaaac ggaataaatg cctatctgca catagacttc tacagactta
    11161 aggaggcaac agtgtggata gacgccgcaa cccagatatt cttcagcctt ggagccggtt
    11221 ttggcgttct gatcgcgttc gcctcttaca acaagttcga caacaactgc tacagggatg
    11281 ctcttctcac ctccagcata aactgtatca cgagcttcgt gagcggattt gccatcttct
    11341 ctatcctggg ctatatggcg catgagcata aggttaacat cgaggacgtg gctaccgagg
    11401 gtgcaggtct tgtgttcatc ctgtacccag aggcaattag caccctgagc ggcagcacct
    11461 tttgggcggt tgtattcttt gtaatgttgt tggctttggg actcgacagc agtatgggtg
    11521 gaatggaagc agtgattacc ggcctggccg acgacttcca ggttctgaag aggcatagga
    11581 agctgttcac gttcggtgtt accttctcaa cgtttctctt ggctctgttc tgcatcacca
    11641 aaggtggcat atacgtgctg accttgctgg atacctttgc agccggcacc tctatactgt
    11701 ttgccgtact gatggaggct atcggtgtga gctggtttta cggggtggac cgattctcta
    11761 acgacatcca acaaatgatg ggctttcgac ccggtctcta ctggcgactg tgctggaaat
    11821 ttgtgtcacc cgccttcctt ctttttgtag ttgtggtaag cataatcaac ttcaagccgc
    11881 tgacgtacga cgattacata ttccccccct gggcgaattg ggtgggctgg ggtatcgcgc
    11941 ttagcagcat ggtgcttgtt cccatctatg tcatctataa gttcctgagc acccagggct
    12001 cactgtggga aaggttggcc tacggcataa cgcccgaaaa cgagcatcat ctggtagccc
    12061 aaagggacat caggcagttc cagctgcaac actggctggc gatctaatag ctcgag
//
