LOCUS       sequence-205798-         530 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of E.coli DHFR protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Cameron CE, Benkovic SJ
  TITLE     Evidence for a functional role of the dynamics of glycine-121 of 
            Escherichia coli dihydrofolate reductase obtained from kinetic 
            analysis of a site-directed mutant.
  JOURNAL   Biochemistry. 1997 Dec 16;36(50):15792-800. doi: 10.1021/bi9716231.
  PUBMED    9398309
REFERENCE   2  (bases 1 to 530)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochemistry"; date: "1997-12-16- 16"; volume: "36"; issue: "50"; 
            pages: "15792-800"
COMMENT     Sequence Label: pET22b-ecDHFR
FEATURES             Location/Qualifiers
     source          1..530
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aggagatata catatgatca gtctgattgc ggcgctagcg gtagatcgcg ttatcggcat
       61 ggaaaacgcc atgccatgga acctgcctgc cgatctcgcc tggtttaaac gcaacacctt
      121 aaataaaccc gtgattatgg ggcgccatac ctgggaatca atcggtaggc ctttgcccgg
      181 ccgcaaaaat attatcctca gcagtcaacc cgggaccgat gatcgggtta cctgggttaa
      241 atcggtcgac gaagccatcg cggcgtgtgg tgacgtacca gaaatcatgg tgattggcgg
      301 cggacgcgtt tatgaacagt tcttgccaaa agcgcaaaaa ctgtatctga cgcatatcga
      361 tgcagaagtg gaaggcgaca cccattttcc ggattacgag ccggatgact gggaatcggt
      421 attcagcgaa ttccacgatg ctgatgcgca gaactcgcat agctattgtt tcgaaatcct
      481 cgagcgtcgt taaggatccg aattcgagct ccgtcgacaa gcttgcggcc
//
