LOCUS       sequence-205801-        9617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TALEN (left) targeting HPRT exon3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, 
            Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K
  TITLE     Microhomology-assisted scarless genome editing in human iPSCs.
  JOURNAL   Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
  PUBMED    29507284
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-5"; volume: "9(1):939. doi"; pages: " 
            10.1038/s41467-018-03044-y"
COMMENT     Sequence Label: CAG-Avr-HPRT-LEFT
FEATURES             Location/Qualifiers
     source          1..9617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..24
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8..24
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    480..496
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     enhancer        523..902
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        904..1179
                     /label=chicken beta-actin promoter
     intron          1180..2192
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2116..2138
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2200..2219
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     2290..2309
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2290..2308
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2316..2358
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      2366..2375
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2375..2440
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             2447..2467
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5093..5680
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     primer_bind     complement(5698..5715)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5704..5815
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5933..5953)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5938..6267
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6118..6253
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6180..6199
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    7469..7590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7506..7525)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7560..7579
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(7643..8434,8435..8503))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8266..8285
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8504..8595)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8637..8656
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8662..8681
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8767..9355
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9256..9275
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9509..9526
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctgtgttgta aaacgacggc cagtcttaag ctcgggcccc ctgggcggtt ctgataacga
       61 gtaatcgtta atccgcaaat aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga
      121 gtttagggaa agagcatttg tcagaatatt taagggcgcc tgtcactttg cttgatatat
      181 gagaattatt taaccttata aatgagaaaa aagcaacgca ctttaaataa gatacgttgc
      241 tttttcgatt gatgaacacc tataattaaa ctattcatct attatttatg attttttgta
      301 tatacaatat ttctagtttg ttaaagagaa ttaagaaaat aaatctcgaa aataataaag
      361 ggaaaatcag tttttgatat caaaattata catgtcaacg ataatacaaa atataataca
      421 aactataaga tgttatcagt atttattatc atttagaata aattttgtgt cgcccttaat
      481 tgtgagcgga taacaattac gagcttcatg cacagtggcg ttgacattga ttattgacta
      541 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
      601 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
      661 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
      721 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      781 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      841 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      901 tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
      961 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg
     1021 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
     1081 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     1141 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gttgccttcg
     1201 ccccgtgccc cgctccgcgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
     1261 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
     1321 taatgacggc tcgtttcttt tctgtggctg cgtgaaagcc ttaaagggct ccgggagggc
     1381 cctttgtgcg ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
     1441 cgcgtgcggc ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     1501 gcgctccgcg tgtgcgcgag gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg
     1561 ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt
     1621 gggcgcggcg gtcgggctgt aacccccccc tgcacccccc tccccgagtt gctgagcacg
     1681 gcccggcttc gggtgcgggg ctccgtgcgg ggcgtggcgc ggggctcgcc gtgccgggcg
     1741 gggggtggcg gcaggtgggg gtgccgggcg gggcggggcc gcctcgggcc ggggagggct
     1801 cgggggaggg gcgcggcggc ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca
     1861 gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct
     1921 gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc
     1981 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     2041 gtccccttct ccctctccag cctcggggct gtccgcgggg ggacggctgc cttcgggggg
     2101 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     2161 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     2221 tgtctcatca ttttggcaaa gaattctctg gctaactaga gaacccactg cttactggct
     2281 tatcgaaatt aatacgactc actataggga agcttcttgt tctttttgca gaagctcaga
     2341 ataaacgctc aactttggcc tcgaggccac catggactat aaggaccacg acggagacta
     2401 caaggatcat gatattgatt acaaagacga tgacgataag atggccccaa agaagaagcg
     2461 gaaggtcggt atccacggag tcccagcagc cgtagatttg agaactttgg gatattcaca
     2521 gcagcagcag gaaaagatca agcccaaagt gaggtcgaca gtcgcgcagc atcacgaagc
     2581 gctggtgggt catgggttta cacatgccca catcgtagcc ttgtcgcagc accctgcagc
     2641 ccttggcacg gtcgccgtca agtaccagga catgattgcg gcgttgccgg aagccacaca
     2701 tgaggcgatc gtcggtgtgg ggaaacagtg gagcggagcc cgagcgcttg aggccctgtt
     2761 gacggtcgcg ggagagctga gagggcctcc ccttcagctg gacacgggcc agttgctgaa
     2821 gatcgcgaag cggggaggag tcacggcggt cgaggcggtg cacgcgtggc gcaatgcgct
     2881 cacgggagca cccctcaacc tgaccccgga ccaggtggtt gcaatcgcgt cacacgatgg
     2941 gggaaagcag gccctagaaa ccgttcagcg actcctgccc gtcctgtgcc aggaccacgg
     3001 cctgaccccg gaacaggtgg ttgcaatcgc gtcacacgat gggggaaagc aggccctaga
     3061 aaccgttcag cgactcctgc ccgtcctgtg ccaggcccac ggcctgaccc cagaccaggt
     3121 tgtggccatc gccagcaaca taggtggcaa gcaggccctc gaaaccgtcc agagactgtt
     3181 accggttctc tgccaggccc acggcctgac ccccgcccag gttgtcgcta ttgctagtaa
     3241 cggcggaggc aaacaggcgc tggaaacagt tcagcgcctc ttgccggtct tgtgtcagga
     3301 ccacggcctg acccccgacc aggttgtcgc tattgctagt aacggcggag gcaaacaggc
     3361 gctggaaaca gttcagcgcc tcttgccggt cttgtgtcag gaccacggcc tgaccccgga
     3421 acaggtggtt gcaatcgcgt cacacgatgg gggaaagcag gccctagaaa ccgttcagcg
     3481 actcctgccc gtcctgtgcc aggcccacgg cctgaccccg gaccaggtgg ttgcaatcgc
     3541 gtcacacgat gggggaaagc aggccctaga aaccgttcag cgactcctgc ccgtcctgtg
     3601 ccaggcccac ggcctgaccc ccgcccaggt tgtcgctatt gctagtaacg gcggaggcaa
     3661 acaggcgctg gaaacagttc agcgcctctt gccggtcttg tgtcaggacc acggcctgac
     3721 cccagaccag gttgtggcca tcgccagcaa cataggtggc aagcaggccc tcgaaaccgt
     3781 ccagagactg ttaccggttc tctgccagga ccacggcctg acccccgaac aggttgtcgc
     3841 tattgctagt aacggcggag gcaaacaggc gctggaaaca gttcagcgcc tcttgccggt
     3901 cttgtgtcag gcccacggcc tgaccccaga ccaagttgtc gcgattgcaa gcaacaacgg
     3961 aggcaaacaa gccttagaaa cagtccagag attgttgcct gtgctgtgcc aagcccacgg
     4021 cctgacccca gcccaggttg tggccatcgc cagcaacata ggtggcaagc aggccctcga
     4081 aaccgtccag agactgttac cggttctctg ccaggaccac ggcctgaccc cggaccaggt
     4141 ggttgcaatc gcgtcacacg atgggggaaa gcaggcccta gaaaccgttc agcgactcct
     4201 gcccgtcctg tgccaggacc acggcctgac ccccgaacag gttgtcgcta ttgctagtaa
     4261 cggcggaggc aaacaggcgc tggaaacagt tcagcgcctc ttgccggtct tgtgtcaggc
     4321 ccacggcctg accccagacc aagttgtcgc gattgcaagc aacaacggag gcaaacaagc
     4381 cttagaaaca gtccagagat tgttgcctgt gctgtgccaa gcccacggcc tgacccccgc
     4441 ccaggttgtc gctattgcta gtaacggcgg aggcaaacag gcgctggaaa cagttcagcg
     4501 cctcttgccg gtcttgtgtc aggaccacgg cctgacccca gaccaggttg tggccatcgc
     4561 cagcaacata ggtggcaagc aggccctcga aaccgtccag agactgttac cggttctctg
     4621 ccaggaccac ggcctgaccc cagaacaagt tgtcgcgatt gcaagcaaca acggaggcaa
     4681 acaagcctta gaaacagtcc agagattgtt gccggtgctg tgccaagccc acggcctgac
     4741 cccagaccag gttgtggcca tcgccagcaa cataggtggc aagcaggccc tcgaaaccgt
     4801 ccagagactg ttaccggttc tctgccaggc ccacggcctg acccctgagc aggtagtggc
     4861 tattgcatcc aacggagggg gcagacccgc actggagtca atcgtggccc agctttcgag
     4921 gccggacccc gcgctggccg cactcactaa tgatcatctt gtagcgctgg cctgcctcgg
     4981 cggacgaccc gccttggatg cggtgaagaa ggggctcccg cacgcgcctg cattgattaa
     5041 gcggaccaac agaaggatcc ccgagaggac atcacatcga gtggcaggtt cccaactcgt
     5101 gaagagtgaa cttgaggaga aaaagtcgga gctgcggcac aaattgaaat acgtaccgca
     5161 tgaatacatc gaacttatcg aaattgctag gaactcgact caagacagaa tccttgagat
     5221 gaaggtaatg gagttcttta tgaaggttta tggataccga gggaagcatc tcggtggatc
     5281 acgaaaaccc gacggagcaa tctatacggt ggggagcccg attgattacg gagtgatcgt
     5341 cgacacgaaa gcctacagcg gtgggtacaa tcttcccatc gggcaggcag atgagatgca
     5401 acgttatgtc gaagaaaatc agaccaggaa caaacacatc aatccaaatg agtggtggaa
     5461 agtgtatcct tcatcagtga ccgagtttaa gtttttgttt gtctctgggc atttcaaagg
     5521 caactataag gcccagctca cacggttgaa tcacattacg aactgcaatg gtgcggtttt
     5581 gtccgtagag gaactgctca ttggtggaga aatgatcaaa gcgggaactc tgacactgga
     5641 agaagtcaga cgcaagttta acaatggcga gatcaatttc cgctcataaa aaatcagcct
     5701 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     5761 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcacaac
     5821 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     5881 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     5941 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     6001 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     6061 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     6121 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6181 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     6241 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     6301 tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg
     6361 atcgaaaagt tcgacagcgt ccgacctgat gcagctctcg gagggcgaag aatctcgtgc
     6421 tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg
     6481 tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga
     6541 agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca
     6601 gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc
     6661 ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt
     6721 cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga
     6781 tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca
     6841 ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca
     6901 cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga
     6961 ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag
     7021 gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct
     7081 tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca
     7141 gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat
     7201 cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt
     7261 ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg
     7321 tccgagggca aaggaatagc acgtgctacg agatttcgat tccaccgccg ccttctatga
     7381 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     7441 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     7501 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7561 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     7621 gagcttggcg taatcatggt cattaccaat gcttaatcag tgaggcacct atctcagcga
     7681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7741 gggagggctt accatctggc cccagcgctg cgatgatacc gcgagaacca cgctcaccgg
     7801 ctccggattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7921 cgccagttaa tagtttgcgc aacgttgttg ccatcgctac aggcatcgtg gtgtcacgct
     7981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     8041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     8101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     8161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     8221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     8281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     8341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     8401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8461 caaaaaaggg aataagggcg acacggaaat gttgaatact catattcttc ctttttcaat
     8521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8581 agaaaaataa acaaataggg gtcagtgtta caaccaatta accaattctg aacattatcg
     8641 cgagcccatt tatacctgaa tatggctcat aacacccctt gctcatgacc aaaatccctt
     8701 aacgtgagtt acgcgcgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8761 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8821 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8881 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttagccca
     8941 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9001 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9061 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9121 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9181 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9241 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9301 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9361 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9421 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9481 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaaggcga
     9541 gagtagggaa ctgccaggca tcaaactaag cagaaggccc ctgacggatg gcctttttgc
     9601 gtttctacaa actcttt
//
