LOCUS       sequence-20581-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG303GAL-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(33..157)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(165..181)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(165..181)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(196..637)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(202..225)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    520..637
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 gtgtaaagcc tgggggtgcc taatgcggcc gccatagtga ctggatatgt tgtgttttac
       61 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
      121 tttacgtttc tcgttcagct tttttgtaca aacttgtgat gggggatcca ctagttctag
      181 aatccggggt tttttctcct tgacgttaaa gtatagaggt atattaacaa ttttttgttg
      241 atacttttat tacatttgaa taagaagtaa tacaaaccga aaatgttgaa agtattagtt
      301 aaagtggtta tgcagttttt gcatttatat atctgttaat agatcaaaaa tcatcgcttc
      361 gctgattaat taccccagaa ataaggctaa aaaactaatc gcattatcat cctatggttg
      421 ttaatttgat tcgttcattt gaaggtttgt ggggccaggt tactgccaat ttttcctctt
      481 cataaccata aaagctagta ttgtagaatc tttattgttc ggagcagtgc ggcgcgaggc
      541 acatctgcgt ttcaggaacg cgaccggtga agacgaggac gcacggagga gagtcttcct
      601 tcggagggct gtcacccgct cggcggcttc taatccgtac ta
//
