LOCUS       sequence-205817-        9504 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV driven GFP based calcium (Ca2+) reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Belote RL, Simon SM
  TITLE     Ca2+ transients in melanocyte dendrites and dendritic spine-like 
            structures evoked by cell-to-cell signaling.
  JOURNAL   J Cell Biol. 2020 Jan 6;219(1). pii: 132739. doi: 
            10.1083/jcb.201902014.
  PUBMED    31821412
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-01-6"; volume: "219(1). pii: 132739. doi"; 
            pages: " 10.1083/jcb.201902014"
COMMENT     Sequence Label: Phi3-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..9504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2659..2679
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2906..2915
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2912..2920,2921..2938,2939..2941,2942..2974,2975..2977,
                     2978..3001,3002..3028,3029..3085,3086..3091,3092..3361,
                     3362..3379,3380..3814,3815..3820,3821..4264)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      3008..3017
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     3298..3319
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3425..3446)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3686..3705)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     promoter        4292..4791
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4327..4350)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4724..4743
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4812..5411
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4812..4831)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5308..5328
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5425..6013
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5478..5498)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5896..5907)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6220..6853
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6982..6998)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6982..6998)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6995..7017)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7006..7022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7030..7036,7037..7054,7055..7060))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7075..7096
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7213..7230)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7384..7972)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7464..7483)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8143..8934,8935..9003))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8766..8785
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9004..9108)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9156..9290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9193..9212)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9247..9266
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tcgctagcgc tgaagcttag atctcgagaa
     2821 ttcgcggccg ctccggacgc gtcgacaatt gggcccatgc atggcatgcc taggccggcg
     2881 ttaacgcgat cgcctgcagg atctcgccac catgggttct catcatcatc atcatcatgg
     2941 tatggctagc atgactggtg gacagcaaat gggtcgggat ctgtacgacg atgacgataa
     3001 ggatctcgcc accatggtcg actcatcacg tcgtaagtgg aataagacag gtcacgcagt
     3061 cagagctata ggtcggctga gctcactcga gaacgtctat atcaaggccg acaagcagaa
     3121 gaacggcatc aaggcgaact tcaagatccg ccacaacatc gaggacggcg gcgtgcagct
     3181 cgcctaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3241 ccactacctg agcgtgcagt ccaaactttc gaaagacccc aacgagaagc gcgatcacat
     3301 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3361 gggcggtacc ggagggagca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     3421 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     3481 tgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     3541 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     3601 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacat
     3661 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     3721 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     3781 cggcaacatc ctggggcaca agctggagta caacctgccg gaccaactga ctgaagagca
     3841 gatcgcagaa tttaaagagg aattctccct atttgacaag gacggggatg ggacaataac
     3901 aaccaaggag ctggggacgg tgatgcggtc tctggggcag aaccccacag aagcagagct
     3961 gcaggacatg atcaatgaag tagatgccga cggtgacggc acaatcgact tccctgagtt
     4021 cctgacaatg atggcaagaa aaatgaaata cagggacacg gaagaagaaa ttagagaagc
     4081 gttcggtgtg tttgataagg atggcaatgg ctacatcagt gcagcagagc ttcgccacgt
     4141 gatgacaaac cttggagaga agttaacaga tgaagaggtt gatgaaatga tcagggaagc
     4201 agacatcgat ggggatggtc aggtaaacta cgaagagttt gtacaaatga tgacagcgaa
     4261 gtgagcggcc gcgactctag ataattctac cgggtagggg aggcgctttt cccaaggcag
     4321 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
     4381 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
     4441 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
     4501 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
     4561 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
     4621 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg
     4681 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
     4741 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc
     4801 ccaagcttac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     4861 ccagggcagt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     4921 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     4981 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     5041 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     5101 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     5161 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     5221 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     5281 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     5341 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     5401 ccggtgcctg accgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg
     5461 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     5521 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     5581 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     5641 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     5701 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     5761 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
     5821 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     5881 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     5941 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     6001 gccgcctccc cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca
     6061 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     6121 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     6181 ctgtagatct tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc
     6241 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     6301 agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca
     6361 agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct
     6421 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga
     6481 ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca
     6541 agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc
     6601 gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt
     6661 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6721 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6781 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6841 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     6901 aaatgaatat cagagagtga gaggccttga cattgctagc gttttaccgt cgacctctag
     6961 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7021 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7081 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7141 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7201 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7261 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7321 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7381 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7441 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7501 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7561 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7621 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7681 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7741 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7801 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7861 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7921 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7981 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8041 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8101 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8161 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     8221 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8281 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8341 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8401 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8461 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8521 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8581 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8641 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8701 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8761 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8821 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8881 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8941 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9001 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9061 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9121 aaaagtgcca cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg
     9181 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9241 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa
     9301 ctcaagctaa ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt
     9361 acctgtggtt tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa
     9421 attgtatttg ttaaatatgt actacaaatt attttcattt taaagaaatt gtatttgtta
     9481 aatatgtact acaaacttag tagt
//
