LOCUS       sequence-205827-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To fuse your protein of interest to the N-terminus of BioID2 and use
            in proximity-dependent biotin identification (BioID); MCS is present
            upstream of BioID2 tag and HA tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Kim DI, Jensen SC, Noble KA, Kc B, Roux KH, Motamedchaboki K, Roux 
            KJ
  TITLE     An improved smaller biotin ligase for BioID proximity labeling.
  JOURNAL   Mol Biol Cell. 2016 Feb 24. pii: mbc.E15-12-0844.
  PUBMED    26912792
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell. 2016 Feb 24. pii: mbc.E15-12-0844."
COMMENT     Sequence Label: MCS-BioID2-HA
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gcttaaggcc tgttaaccgg tcgtacgtct
      121 ccggattcga attcggatcc ttcaagaacc tgatctggct gaaggaggtg gacagcaccc
      181 aggagagact gaaggagtgg aacgtgagct acggcaccgc cctggtggcc gacagacaga
      241 ccaagggcag aggcggcctg ggcagaaagt ggctgagcca ggagggcggc ctgtacttca
      301 gcttcctgct gaaccccaag gagttcgaga acctgctgca gctgcccctg gtgctgggcc
      361 tgagcgtgag cgaggccctg gaggagatca ccgagatccc cttcagcctg aagtggccca
      421 acgacgtgta cttccaggag aagaaggtga gcggcgtgct gtgcgagctg agcaaggaca
      481 agctgatcgt gggcatcggc atcaacgtga accagagaga gatccccgag gagatcaagg
      541 acagagccac caccctgtac gagatcaccg gcaaggactg ggacagaaag gaggtgctgc
      601 tgaaggtgct gaagagaatc agcgagaacc tgaagaagtt caaggagaag agcttcaagg
      661 agttcaaggg caagatcgag agcaagatgc tgtacctggg cgaggaggtg aagctgctgg
      721 gcgagggcaa gatcaccggc aagctggtgg gcctgagcga gaagggcggc gccctgatcc
      781 tgaccgagga gggcatcaag gagatcctga gcgg
//
