LOCUS       sequence-205830-        8765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HPRT1 c.312C>A (Munich) donor vector with bilateral microhomology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, 
            Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K
  TITLE     Microhomology-assisted scarless genome editing in human iPSCs.
  JOURNAL   Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
  PUBMED    29507284
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-5"; volume: "9(1):939. doi"; pages: " 
            10.1038/s41467-018-03044-y"
COMMENT     Sequence Label: p3-HPRT1-S104Rf-PdTK-mCh
FEATURES             Location/Qualifiers
     source          1..8765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..26
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             1025..1078
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1088..1684
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1088..1107)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1584..1604
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1691..2686
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(2718..2735)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2724..2948
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        2967..3346
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3348..3625
                     /label=chicken beta-actin promoter
     intron          3626..4641
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4565..4587
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4649..4668
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    4735..4759
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4765..5475
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4910..4928)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5178..5197
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5372..5395
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(5477..5501)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5597..5616)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5662..5717
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5716..5735)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     6490..6507
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6491..6507
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6528..6551)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     primer_bind     complement(6631..6648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6802..7390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6882..6901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7561..8352,8353..8421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8184..8203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8422..8526)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8594..8612
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8650..8672)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
       61 acagatgcgt aaggagcgat cgcgaaaata ccgcatcagg cgtgcagtgc agcagaatga
      121 tcacagttca ctgcagtctc aacctcctgg gttcaagcga tccttccacc tcagcctcct
      181 gagtagctgg gactataggt gtgcgccacc actcccagct aatattttta ttttgtagaa
      241 acagggttcg ccatgttacc cagcctccca aagtgctggg attacaggca tgagccactg
      301 gcccagtttc tacagtctct cttaatattg tatattatcc aagaaatttc atttaatcag
      361 aacctgccag tctgataggt gaaaatggta tcttgttttt atttgcattt aaaaaaaatt
      421 atgatagtgg tatgcttggt ttttttgaag gtatcaaatt ttttacctta tgaaacatga
      481 gggcaaagga tgtgttacgt ggaagattta aaaaaaattt ttaatgcatt tttttgagac
      541 aaggtcttgc tctattgtcc aggctggagt gcagtggcac aatcacagtt cactccagcc
      601 tcaacatcct gcactaaagt gattttccca cctcacctct caagtagctg ggactacagg
      661 tacatgctac catgcctggc taattttttt ttttttgcag gcatggggtc tcactatatt
      721 gcccaggttg gtgtggaagt ttaatgacta agaggtgttt gttataaagt ttaatgtatg
      781 aaactttcta ttaaattcct gattttattt ctgtaggact gaacgtcttg ctcgagatgt
      841 gatgaaggag atgggaggcc atcacattgt agccctctgt gtgctcaagg ggggctataa
      901 attctttgct gacctgctgg attacatcaa agcactgaat agaaatagtg atagatccat
      961 tcctatgact gtagatttta tcagacttaa gagatattgt accaccggca agctgcccgt
     1021 gcccgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctag
     1081 gctcgagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     1141 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     1201 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     1261 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     1321 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     1381 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     1441 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     1501 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     1561 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     1621 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     1681 tgccggatcc atgcccacgc tactgcgggt ttatatagac ggtccccacg ggatggggaa
     1741 aaccaccacc acgcaactgc tggtggccct gggttcgcgc gacgatatcg tctacgtacc
     1801 cgagccgatg acttactggc gggtgctggg ggcttccgag acaatcgcga acatctacac
     1861 cacacaacac cgcctcgacc agggtgagat atcggccggg gacgcggcgg tggtaatgac
     1921 aagcgcccag ataacaatgg gcatgcctta tgccgtgacc gacgccgttc tggctcctca
     1981 tatcgggggg gaggctggga gctcacatgc cccgcccccg gccctcaccc tcatcttcga
     2041 ccgccatccc atcgccgccc tcctgtgcta cccggccgcg cggtacctta tgggcagcat
     2101 gaccccccag gccgtgctgg cgttcgtggc cctcatcccg ccgaccttgc ccggcaccaa
     2161 catcgtgctt ggggcccttc cggaggacag acacatcgac cgcctggcca aacgccagcg
     2221 ccccggcgag cggctggacc tggctatgct ggctgcgatt cgccgcgttt acgggctact
     2281 tgccaatacg gtgcggtatc tgcagtgcgg cgggtcgtgg cgggaggact ggggacagct
     2341 ttcggggacg gccgtgccgc cccagggtgc cgagccccag agcaacgcgg gcccacgacc
     2401 ccatatcggg gacacgttat ttaccctgtt tcgggccccc gagttgctgg cccccaacgg
     2461 cgacctgtat aacgtgtttg cctgggcctt ggacgtcttg gccaaacgcc tccgttccat
     2521 gcacgtcttt atcctggatt acgaccaatc gcccgccggc tgccgggacg ccctgctgca
     2581 acttacctcc gggatggtcc agacccacgt caccaccccc ggctccatac cgacgatatg
     2641 cgacctggcg cgcacgtttg cccgggagat gggggaggct aactgatcta gagggcccgt
     2701 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     2761 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     2821 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     2881 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     2941 ctctatggac tagcggtggc ggcctcgaca ttgattattg actagttatt aatagtaatc
     3001 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     3061 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     3121 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     3181 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     3241 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     3301 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtcg aggtgagccc
     3361 cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt tgtatttatt
     3421 tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggggc gcgcgccagg
     3481 cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa
     3541 tcagagcggc gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta
     3601 taaaaagcga agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg
     3661 ctccgcgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg
     3721 agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt
     3781 gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg
     3841 gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc
     3901 gcgctgcccg gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt
     3961 gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg
     4021 aacaaaggct gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg
     4081 gtcgggctgc aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg
     4141 ggtgcggggc tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg
     4201 caggtggggg tgccgggcgg ggcggggccg cctcgggccg gggagggctc gggggagggg
     4261 cgcggcggcc cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt
     4321 ttatggtaat cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg
     4381 aaatctggga ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc
     4441 ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc
     4501 cctctccagc ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg
     4561 gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat
     4621 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
     4681 tttggcaaag aattcgcggc cgcggtaccg gcgcgccgga tcctgcaggt tatcacaagt
     4741 ttgtacaaaa aagcaggctc caccatggtg agcaagggcg aggaggacaa catggccatc
     4801 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     4861 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     4921 gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     4981 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
     5041 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     5101 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     5161 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     5221 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     5281 aaggacggcg gccactacga cgccgaggtc aagaccacct acaaggccaa gaagcccgtg
     5341 cagctgcccg gcgcctacaa cgtcaacatc aagctggaca tcacctccca caacgaggac
     5401 tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg cggcatggac
     5461 gagctgtaca agtaagaccc agctttcttg tacaaagtgg tgataactct agagaattca
     5521 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     5581 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     5641 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     5701 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     5761 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     5821 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     5881 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     5941 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     6001 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     6061 agcttggatc cctcgagtta attaaatcta gaagtcgacg ggcacgggca gcttgccggt
     6121 ggtaagagat attgtgtgag tatatttaat atatgattct ttttagtggc aacagtaggt
     6181 tttcttatat tttctttgaa tctctgcaaa ccatacttgc tttcatttca cttggttaca
     6241 gtgagatttt tctaacatat tcactagtac tttacatcaa agccaatact gtttttttaa
     6301 aactagtcac cttggaggat atatacttat tttacaggtg tgtgtggttt tttaaataaa
     6361 ctccttttag gaattgctgt tgggacttgg gatacttttt tcactataca tactggtgac
     6421 agataccctc tcttgagcta catcggtttg tggggagtca aaagtccttt ggagctaggt
     6481 ttgacaaatt gtaaaacgac ggccagtgcg gccgcgaatt cggtacccta actaacagga
     6541 agagtttgta ggggcccccc ctcgactgca ttaatgaatc ggccaacgcg cggggagagg
     6601 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     6661 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     6721 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     6781 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6841 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6901 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6961 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7021 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     7081 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     7141 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     7201 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     7261 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     7321 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7381 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7441 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7501 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7561 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7621 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7681 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7741 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7801 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7861 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7921 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7981 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8041 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8101 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8161 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8221 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8281 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8341 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8401 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8461 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8521 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     8581 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     8641 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     8701 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     8761 gctta
//
