LOCUS       sequence-205833-        2862 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC7
FEATURES             Location/Qualifiers
     source          1..2862
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     91..108
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(837..855)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        923..1027
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1249..2040
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2211..2799
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2700..2719
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
       61 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatc actctgtggt
      181 ctcaaatgtg ttctcataat gaaaacatcg attcccgaaa attcacaaaa tcagaacctt
      241 gtcatattaa aaaaatctca atttcaattc tctcgatttc tctatcatca tcatccatgg
      301 cttcgcaagc tagccttctc cttcagaaac aactcaaaga tctctgtaag catcctgttg
      361 atggattctc tgctggactc gttgatgaga agaatatatt cgaatggagc gttaccatta
      421 tcggacctcc tgatactctc tatgaaggag gattctttaa cgcgattatg acatttccgc
      481 aaaactatcc gaatagtccg ccaaccgtga ggtttacttc ggatatgtgg catcctaacg
      541 tttattctga tggtcgtgtt tgcatatcta ttcttcatcc tcccggtgat gatcctagcg
      601 gttatgagct tgcaagcgag cgttggactc ctgttcatac agttgagagt attatgttga
      661 gtatcatatc aatgctttct ggtcctaacg atgagtctcc agcaaatgtt gaagctgcca
      721 aagaatggcg agataagaga gatgagttca agaagaaagt gagccgttgt gtcagaaagt
      781 ctcaagaaat gttttgagct ttgagaccac gaagtggctc ttcagtggac gaaagggcct
      841 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
      901 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      961 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     1021 gaagagtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     1081 cgcagtggcg gttttcatgg cttgttatga ctgttttttt ttggggtaca gtctatgcct
     1141 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc
     1201 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg
     1261 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc
     1321 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca
     1381 cacagcgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga
     1441 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc
     1501 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag
     1561 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca
     1621 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc
     1681 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag
     1741 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga
     1801 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg
     1861 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata
     1921 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat
     1981 cagttggaag aatttgtcca ttacgtaaaa ggcgagatca ccaaggtagt cggcaaataa
     2041 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     2101 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     2161 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2221 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2281 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2341 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2401 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2461 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2521 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2581 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2641 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2701 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2761 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2821 ttacggttcc tggccttttg ctggcctttt gctcacatgt tc
//
