LOCUS       sequence-20584-e         711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 711)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG303GPD-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(10..134)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(142..158)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(142..158)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(164..427)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(247..270)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
       61 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttt
      121 ttgtacaaac ttgtgatggg ggatccacta gttctagaat ccgtcgaaac taagttctgg
      181 tgttttaaaa ctaaaaaaaa gactaactat aaaagtagaa tttaagaagt ttaagaaata
      241 gatttacaga attacaatca atacctaccg tctttatata cttattagtc aagtagggga
      301 ataatttcag ggaactggtt tcaacctttt ttttcagctt tttccaaatc agagagagca
      361 gaaggtaata gaaggtgtaa gaaaatgaga tagatacatg cgtgggtcaa ttgccttgtg
      421 tcatcattta ctccaggcag gttgcatcac tccattgagg ttgtgcccgt tttttgcctg
      481 tttgtgcccc tgttctctgt agttgcgcta agagaatgga cctatgaact gatggttggt
      541 gaagaaaaca atattttggt gctgggattc tttttttttc tggatgccag cttaaaaagc
      601 gggctccatt atatttagtg gatgccagga ataaactgtt cacccagaca cctacgatgt
      661 tatatattct gtgtaacccg ccccctattt tgggcatgta cgggttacag c
//
