LOCUS       sequence-205841-        7954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  puro-deltaTK gene-trap selection cassette (AAVS1 donor vector 
            backbone) with constitutive mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7954)
  AUTHORS   Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, 
            Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K
  TITLE     Microhomology-assisted scarless genome editing in human iPSCs.
  JOURNAL   Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
  PUBMED    29507284
REFERENCE   2  (bases 1 to 7954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-5"; volume: "9(1):939. doi"; pages: " 
            10.1038/s41467-018-03044-y"
COMMENT     Sequence Label: pAAVS1-PdTK-CAG-mCh-[uBglII]
FEATURES             Location/Qualifiers
     source          1..7954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             124..834
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(269..287)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     537..556
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     731..754
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(836..860)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(960..979)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1025..1080
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1079..1098)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     misc_feature    1636..2165
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(2311..2330)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2312..2330)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2337..2354)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2337..2353)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2346..2368)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2495..2950
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2582..2601)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2792..2813
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2976..3080
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3081..3149,3150..3941)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3299..3318)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4112..4700
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4601..4620
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4854..4871
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4988..5009
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5024..5029,5030..5047,5048..5054)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5062..5078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5067..5089
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5086..5102
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5086..5102
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5123..5141
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    5154..5957
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    5964..5989
                     /label=SA
                     /note="splice acceptor site"
     CDS             6013..6066
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6076..6672
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6076..6095)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6572..6592
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6679..7674
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(7706..7723)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7712..7936
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgcggcc
       61 gcggtaccgg cgcgccggat cctgcaggtt atcacaagtt tgtacaaaaa agcaggctcc
      121 accatggtga gcaagggcga ggaggacaac atggccatca tcaaggagtt catgcgcttc
      181 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      241 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
      301 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      361 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      421 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
      481 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      541 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      601 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      661 gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      721 gtcaacatca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      781 gagcgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagaccca
      841 gctttcttgt acaaagtggt gataactcta gagaattcac tcctcaggtg caggctgcct
      901 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgat
      961 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     1021 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     1081 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     1141 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     1201 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     1261 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     1321 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     1381 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg atccctcgag
     1441 ttaattaaat ctagaagtcg acagtactaa gctttgacag aaaagcccca tccttaggcc
     1501 tcctccttcc tagtctcctg atattgggtc taacccccac ctcctgttag gcagattcct
     1561 tatctggtga cacaccccca tttcctggag ccatctctct ccttgccaga acctctaagg
     1621 tttgcttacg atggagccag agaggatcct gggagggaga gcttggcagg gggtgggagg
     1681 gaaggggggg atgcgtgacc tgcccggttc tcagtggcca ccctgcgcta ccctctccca
     1741 gaacctgagc tgctctgacg cggctgtctg gtgcgtttca ctgatcctgg tgctgcagct
     1801 tccttacact tcccaagagg agaagcagtt tggaaaaaca aaatcagaat aagttggtcc
     1861 tgagttctaa ctttggctct tcacctttct agtccccaat ttatattgtt cctccgtgcg
     1921 tcagttttac ctgtgagata aggccagtag ccagccccgt cctggcaggg ctgtggtgag
     1981 gaggggggtg tccgtgtgga aaactccctt tgtgagaatg gtgcgtccta ggtgttcacc
     2041 aggtcgtggc cgcctctact ccctttctct ttctccatcc ttctttcctt aaagagtccc
     2101 cagtgctatc tgggacatat tcctccgccc agagcagggt cccgcttccc taaggccctg
     2161 ctctgggctt ctgggtttga gtccttggca agcccaggag aggcgctcag gcttccctgt
     2221 cccccttcct cgtccaccat ctcatgcccc tggctctcct gccccttccc tacaggggtt
     2281 cctggctctg ctctagcgat cgccaattcg ccctatagtg agtcgtatta caattcactg
     2341 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     2401 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     2461 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     2521 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     2581 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     2641 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     2701 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     2761 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     2821 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     2881 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     2941 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3001 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3061 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3121 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3181 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3241 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3301 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     3361 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3421 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3481 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     3541 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     3601 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     3661 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     3721 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     3781 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     3841 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     3901 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     3961 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4021 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4081 caaaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4141 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4201 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     4261 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4321 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4381 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4441 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4501 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4561 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4621 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4681 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4741 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4801 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4861 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4921 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     4981 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     5041 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     5101 accatgatta cgccaagctc gaaattaacc ctcactaaag ggaacaaaag ctgtgctttc
     5161 tctgaccagc attctctccc ctgggcctgt gccgctttct gtctgcagct tgtggcctgg
     5221 gtcacctcta cggctggccc agatccttcc ctgccgcctc cttcaggttc cgtcttcctc
     5281 cactccctct tccccttgct ctctgctgtg ttgctgccca aggatgctct ttccggagca
     5341 cttccttctc ggcgctgcac cacgtgatgt cctctgagcg gatcctcccc gtgtctgggt
     5401 cctctccggg catctctcct ccctcaccca accccatgcc gtcttcactc gctgggttcc
     5461 cttttccttc tccttctggg gcctgtgcca tctctcgttt cttaggatgg ccttctccga
     5521 cggatgtctc ccttgcgtcc cgcctcccct tcttgtaggc ctgcatcatc accgtttttc
     5581 tggacaaccc caaagtaccc cgtctccctg gctttagcca cctctccatc ctcttgcttt
     5641 ctttgcctgg acaccccgtt ctcctgtgga ttcgggtcac ctctcactcc tttcatttgg
     5701 gcagctcccc tacccccctt acctctctag tctgtgctag ctcttccagc cccctgtcat
     5761 ggcatcttcc aggggtccga gagctcagct agtcttcttc ctccaacccg ggcccctatg
     5821 tccacttcag gacagcatgt ttgctgcctc cagggatcct gtgtccccga gctgggacca
     5881 ccttatattc ccagggccgg ttaatgtggc tctggttctg ggtactttta tctgtcccct
     5941 ccaccccaca gtggggcaag cttctgacct cttctcttcc tcccacaggg cctcgagaga
     6001 tctggcagcg gagagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc
     6061 ggccctaggc tcgagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6121 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6181 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6241 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6301 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     6361 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6421 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6481 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     6541 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6601 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6661 aagcccggtg ccggatccat gcccacgcta ctgcgggttt atatagacgg tccccacggg
     6721 atggggaaaa ccaccaccac gcaactgctg gtggccctgg gttcgcgcga cgatatcgtc
     6781 tacgtacccg agccgatgac ttactggcgg gtgctggggg cttccgagac aatcgcgaac
     6841 atctacacca cacaacaccg cctcgaccag ggtgagatat cggccgggga cgcggcggtg
     6901 gtaatgacaa gcgcccagat aacaatgggc atgccttatg ccgtgaccga cgccgttctg
     6961 gctcctcata tcggggggga ggctgggagc tcacatgccc cgcccccggc cctcaccctc
     7021 atcttcgacc gccatcccat cgccgccctc ctgtgctacc cggccgcgcg gtaccttatg
     7081 ggcagcatga ccccccaggc cgtgctggcg ttcgtggccc tcatcccgcc gaccttgccc
     7141 ggcaccaaca tcgtgcttgg ggcccttccg gaggacagac acatcgaccg cctggccaaa
     7201 cgccagcgcc ccggcgagcg gctggacctg gctatgctgg ctgcgattcg ccgcgtttac
     7261 gggctacttg ccaatacggt gcggtatctg cagtgcggcg ggtcgtggcg ggaggactgg
     7321 ggacagcttt cggggacggc cgtgccgccc cagggtgccg agccccagag caacgcgggc
     7381 ccacgacccc atatcgggga cacgttattt accctgtttc gggcccccga gttgctggcc
     7441 cccaacggcg acctgtataa cgtgtttgcc tgggccttgg acgtcttggc caaacgcctc
     7501 cgttccatgc acgtctttat cctggattac gaccaatcgc ccgccggctg ccgggacgcc
     7561 ctgctgcaac ttacctccgg gatggtccag acccacgtca ccacccccgg ctccataccg
     7621 acgatatgcg acctggcgcg cacgtttgcc cgggagatgg gggaggctaa ctgatctaga
     7681 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7741 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7801 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     7861 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     7921 gcggtgggct ctatggacta gcggtggcgg cctc
//
