LOCUS       sequence-205844-        2700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC12
FEATURES             Location/Qualifiers
     source          1..2700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     122..139
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(706..724)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        792..896
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1118..1909
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2080..2668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2569..2588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
       61 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
      121 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
      181 gcgttggccg attcattaat cactctgtgg tctcaaatgg cttctaagag gatttcaagg
      241 gaactgaggg atatgcagag acatcctcca gcaaactgta gcgcaggtcc tgtggctgag
      301 gaggacatat tccattggca agcaactatc atgggaccgc atgacagtcc gtattccgga
      361 ggcgtattta cggtttctat tgatttctct tcggattatc ctttcaagcc accaaaggtg
      421 aatttcaaga ctaaggtgta ccacccaaac atcgacagca aaggaagcat ttgccttgac
      481 atattgaaag agcaatggag tcctgctcct accacatcca aggttttgtt gtcgatttgc
      541 tcgctgctga ctgacccgaa cccaaatgat cctcttgtgc cggagatagc tcatctctac
      601 aaggtagata aatcaaaata cgagtcaact gcacaaaaat ggacacagaa gtacgcgatg
      661 ggatgagctt tgagaccacg aagtggctct tcagtggacg aaagggcctc gtgatacgcc
      721 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      781 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
      841 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatgc
      901 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
      961 ttttcatggc ttgttatgac tgtttttttt tggggtacag tctatgcctc gggcatccaa
     1021 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     1081 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga
     1141 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     1201 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagcgatat
     1261 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     1321 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     1381 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     1441 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     1501 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     1561 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     1621 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     1681 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     1741 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     1801 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1861 atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac tgtcagacca
     1921 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1981 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     2041 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2101 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2161 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2221 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2281 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2341 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2401 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2461 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2521 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2581 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2641 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
//
