LOCUS       sequence-205846-        2751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC13
FEATURES             Location/Qualifiers
     source          1..2751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     137..154
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(772..790)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        858..962
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1184..1975
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2146..2734
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2635..2654
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
       61 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
      121 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
      181 accgcctctc cccgcgcgtt ggccgattca ttaatcactc tgtggtctca aatgaactca
      241 caagcttgtc tacttcttca gaaacagctt aaagatcttt gtaagcatcc tgtagatgga
      301 ttctctgctg gtttagttga tgagaagaat atattcgaat ggagcgtaac tattattgga
      361 cctcctgata ctctctatga aggaggattt ttctatgcca ttatgtcatt cccacaaaac
      421 tatcctaata gcccacctac ggtgcggttt acttcagata tttggcatcc taacgtttat
      481 cccgatggcc gcgtttgtat atctattcta catcctcctg gtgatgatcc aagtggatat
      541 gagcttgcaa gtgagcgttg gacgcctgtt cacacagttg agagtatcat gttgagtatt
      601 atatcaatgc tctctggtcc caacgacgaa tctccagcaa acgttgaggc tgctaaagaa
      661 tggagagaga agagagatga gttcaagaag aaagtgagcc gttgtgtcag aaagtctcaa
      721 gaaatgtttt gagctttgag accacgaagt ggctcttcag tggacgaaag ggcctcgtga
      781 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      961 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     1021 tggcggtttt catggcttgt tatgactgtt tttttttggg gtacagtcta tgcctcgggc
     1081 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     1141 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat
     1201 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     1261 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     1321 cgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     1381 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     1441 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     1501 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     1561 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     1621 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     1681 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     1741 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     1801 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     1861 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     1921 ggaagaattt gtccattacg taaaaggcga gatcaccaag gtagtcggca aataactgtc
     1981 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2041 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2101 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2161 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2221 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2281 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2341 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2401 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2461 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2521 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2581 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2641 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2701 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg c
//
