LOCUS       sequence-205848-        2736 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC15
FEATURES             Location/Qualifiers
     source          1..2736
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     124..141
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(744..762)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        830..934
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1156..1947
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2118..2706
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2607..2626
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
       61 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
      121 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
      181 gcgcgttggc cgattcatta atcactctgt ggtctcaaat gaccagctct tccgctcctt
      241 cacgcaaggc tttaagcaag atcgcgtgta ataggttgca gaaagagctt tctgagtggc
      301 aattgaatcc ccccactgga tttcgtcaca aagtcactga taatcttcaa aaatggacaa
      361 tagatgttac cggagctcca gggacacttt acgctaatga gacttatcag cttcaggttg
      421 aatttcctga acattaccct atggaagcac cccaggtagt gtttgtttct ccggcacctt
      481 cacatccaca tatatacagc aatggacata tttgtttaga tattctatat gactcatggt
      541 caccagcaat gacagtgaat tcagtctgca tcagcattct ttcgatgcta tcaagttcac
      601 ccgcaaagca acgccctgcg gataacgatc gttatgtgaa gaactgtaag aatgggaggt
      661 cacctaagga gacgaggtgg tggttccatg acgacaaggt ttgagctttg agaccacgaa
      721 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      781 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      841 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      901 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      961 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     1021 tttttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     1081 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     1141 acaaagttaa acatcatgag ggaagcggtg atcgccgaag tatcgactca actatcagag
     1201 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     1261 tccgcagtgg atggcggcct gaagccacac agcgatattg atttgctggt tacggtgacc
     1321 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     1381 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     1441 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     1501 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     1561 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     1621 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     1681 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     1741 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     1801 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     1861 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccatta cgtaaaaggc
     1921 gagatcacca aggtagtcgg caaataactg tcagaccaag tttactcata tatactttag
     1981 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2041 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2101 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2161 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2221 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2281 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2341 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2401 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2461 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2521 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2581 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2641 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2701 tggaaaaacg ccagcaacgc ggccttttta cggttc
//
