LOCUS       sequence-205853-        6349 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid vector expressing human p110 CUX1 (amino acids 878-1505 of 
            P39880), with an N-terminal Myc tag and a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Truscott M, Harada R, Vadnais C, Robert F, Nepveu A
  TITLE     p110 CUX1 cooperates with E2F transcription factors in the 
            transcriptional activation of cell cycle-regulated genes.
  JOURNAL   Mol Cell Biol. 2008 May;28(10):3127-38. doi: 10.1128/MCB.02089-07. 
            Epub 2008 Mar 17.
  PUBMED    18347061
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.02089-07"; journalName: "Mol Cell Biol"; date: 
            "2008-05"; volume: "28"; issue: "10"; pages: "3127-38"
COMMENT     Sequence Label: pXJ42-p110 CUX1
FEATURES             Location/Qualifiers
     source          1..6349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        41..344
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        345..548
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     499..519
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     545..569
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          803..1375
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1383..1402
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     1430..1449
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1430..1448
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1473..1482
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1482..1511
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3414..3440
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    3474..3595
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3511..3530)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3565..3584
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3629..3646)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3800..4388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3880..3899)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4559..5350,5351..5419))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5182..5201
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5420..5524)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5592..5610
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5648..5670)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5770..5789
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(5856..6311)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5993..6014)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6205..6224
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gatcggtgcg ggcctcttcg ctattacgcc agctagagtc cgttacataa cttacggtaa
       61 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      121 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      181 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      241 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      301 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      361 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      421 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      481 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      541 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
      601 tccatagaag acaccgggac cgatccagcc tccgcggccg ggaacggtgc attggaacgg
      661 acccctaggc ttttgcaaaa agctccggat cgatcctgag aacttcaggg tgagtttggg
      721 gacccttgrk yskkswwysk tkywykskaw ygkammmytm rkcttttgca aaaagctccg
      781 gatcgatcct gagaacttca gggtgagttt ggggaccctt gattgttctt tctttttcgc
      841 tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt ttagaatggg
      901 aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc actttctact
      961 ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt tttcgttaaa
     1021 ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt cagattgtaa
     1081 gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt gtacttcagc
     1141 acagttttag agaacaattg ttataattaa atgataaggt agaatatttc tgcatataaa
     1201 ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacatcctgg tcatcatcct
     1261 gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa aatactctga
     1321 gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1381 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattgt aatacgactc
     1441 actatagggc gaattagatc tggtaccctc gagccaccat ggagcagaag ctcatctctg
     1501 aagaggacct cacgcgtggt cgacagaatt actggaagga gtggcccagc gctgagtccc
     1561 catactccca gagctcagag ctgagtctga ccggggccag ccgcagcgag acaccacaga
     1621 acagccccct gccatcctcc ccgatcgtgc ccatgtccaa gcccaccaag ccctcggtcc
     1681 ccccgctgac ccccgagcag tacgaggtct acatgtacca ggaggtggac accatcgagc
     1741 tcacccggca ggttaaggaa aagctggcca agaacggcat ctgccagaga atcttcgggg
     1801 agaaggtgct gggcctgtcc cagggcagcg tcagcgacat gctgtcccga ccgaagccat
     1861 ggagcaagct gacgcagaaa ggccgagaac ccttcatccg gatgcagctc tggctgaacg
     1921 gcgagctagg ccagggtgtt ctacccgtcc agggccagca gcaagggcca gtcctccact
     1981 ccgtgacatc gctccaggac ccgctgcagc agggctgtgt gagctcagaa agcactccaa
     2041 agacctccgc cagctgcagc cctgcccctg agtccccgat gagttccagt gagtcggtga
     2101 agagcctgac cgagctggtc cagcagccct gtccccccat cgaggcgagc aaggacagca
     2161 agccaccaga gcccagtgac ccgccagcat ccgactccca gcccacaacc ccgctgcctc
     2221 tctccggaca ctcggccctc agcatccaag aattagtagc catgtccccg gagctggaca
     2281 cctacggcat aaccaagcgg gtgaaggagg tgctgacgga caacaacctc ggccagcgct
     2341 tatttgggga gaccatctta gggctcaccc aaggctctgt ctctgacctc cttgcccgcc
     2401 ccaaaccctg gcataagctc agtctgaaag gacgagagcc cttcgtccgg atgcagctgt
     2461 ggctgaacga ccccaacaat gtggagaagc tgatggacat gaaacggatg gagaagaaag
     2521 cctacatgaa gcggcggcac agctcagtca gtgacagcca gccctgcgaa ccgccctctg
     2581 tcggcaccga gtacagccag ggcgccagcc cccagcccca gcaccagctg aagaaacccc
     2641 gggtggtgct ggctccggag gagaaggagg cgctgaaacg agcgtatcag caaaagccat
     2701 acccgtcacc aaaaaccatc gaagacctcg ccacccagct caacctgaaa accagcaccg
     2761 tcatcaactg gttccacaac tacaggtctc ggatccgcag agaactgttc attgaggaaa
     2821 ttcaggccgg gagtcagggc caggcgggcg ccagcgactc accctcggcc cgcagcggcc
     2881 gggcggcgcc cagctcggag ggcgacagct gcgacggcgt ggaggccact gagggcccag
     2941 gcagcgccga caccgaggag cccaagtctc agggagaggc cgagcgggag gaggtgccgc
     3001 ggccggcgga gcagacggag ccgccgccct cggggacccc gggcccggac gacgcccgcg
     3061 acgacgacca cgagggaggc cccgtggaag gcccggggcc cctgcccagc cccgcctccg
     3121 cgaccgccac cgccgcgccc gcggcccccg agcasgccgc tacctcagcc gccgccscgc
     3181 cgggggaggg ccccgcggcc ccgagctccg cgccgccgcc cagcaacagc agcagcagca
     3241 gcgccccccg caggcccagc tcgctgcaga gccttttcgg cctccccgag gccgcgggcg
     3301 cccgggactc gcgcgacaac cccctgcgca agaagaaggc cgcgaacttg aacagcatca
     3361 tccaccgcct ggagaaggcc gccagccggg aggaacctat cgaatgggag ttctatccat
     3421 acgacgtacc tgactacgca tgagcggccg cgaattcgat cttattaaag cagaacttgt
     3481 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     3541 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     3601 tctggtcgac tctagactct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     3661 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     3721 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3781 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3841 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3901 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3961 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4021 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4081 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4141 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4201 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     4261 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4321 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4381 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     4441 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     4501 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     4561 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     4621 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     4681 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     4741 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     4801 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     4861 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     4921 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     4981 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5041 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5101 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5161 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5221 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5281 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5341 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     5401 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     5461 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     5521 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     5581 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     5641 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     5701 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     5761 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     5821 cgcacagatg cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt
     5881 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     5941 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     6001 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     6061 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     6121 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     6181 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     6241 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     6301 acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggc
//
