LOCUS       sequence-205856-        9327 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9327)
  AUTHORS   Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M
  TITLE     Direct conversion of fibroblasts to functional neurons by defined 
            factors.
  JOURNAL   Nature. 2010 Feb 25. 463(7284):1035-41.
  PUBMED    20107439
REFERENCE   2  (bases 1 to 9327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2010 Feb 25. 463(7284):1035-41."
COMMENT     Sequence Label: Tet-O-FUW-Olig2
FEATURES             Location/Qualifiers
     source          1..9327
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        53..432
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        434..632
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             650..830
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    877..1002
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1495..1728
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1913..1957
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2255..2372
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2429..2447
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2471..2489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2513..2531
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2555..2573
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2597..2615
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2639..2657
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2690..2728
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2725..2749
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2832..2841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3834..4422
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3887..3907)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4305..4316)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4425..4441)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4426..4442)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4947..5127
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5153..5170)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5159..5383
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5429..5857
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5516..5535)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5726..5747
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5866..5886)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5871..6200
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6051..6186
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6113..6132
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6248..6295
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6314..6688
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6818..6939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6855..6874)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6909..6928
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6988..7004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6988..7004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7001..7023)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7012..7028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7036..7042,7043..7060,7061..7066))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7081..7102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7219..7236)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7390..7978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7470..7489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8149..8940,8941..9009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8772..8791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9010..9114)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9189..9208)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
       61 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      121 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      181 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      241 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      301 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      361 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      421 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      481 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      541 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      601 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
      661 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      721 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      781 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      841 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      901 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      961 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1021 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1081 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1141 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1201 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1261 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1321 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1381 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1441 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1501 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1561 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1621 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1681 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1741 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1801 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1861 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1921 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1981 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2041 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2101 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2161 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2221 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2281 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2341 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2401 cagtttggtt agatctcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2461 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     2521 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     2581 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     2641 cctatcagtg atagagaaaa gtgaaagtcg agctcggtac ccgggtcgag gtaggcgtgt
     2701 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
     2761 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggccc
     2821 cgaattcgct agccaccatg gactcggacg ccagcctggt gtctagtcgc ccatcgtccc
     2881 cagaacccga tgatcttttt ctgcccgccc ggagcaaggg gggaagcagc agcggcttca
     2941 caggagggac tgtgtcctcg tccacgccga gcgactgccc gccagagctg agctccgagc
     3001 tacgaggggc catgggcgcg tcaggcgcgc atcccgggga caaactgggc ggcggtggct
     3061 tcaagtcatc ttcctccagc acctcctcgt ccacgtcctc ggcggccacg tcttccacca
     3121 agaaagacaa gaagcagatg actgagcccg agctgcagca gctgcgcctg aagatcaaca
     3181 gccgcgaacg caagcgcatg cacgacctca acatcgccat ggacggcctg cgggaggtca
     3241 tgccttacgc gcacgggccg tcggtgcgca agctctccaa gatcgccacg ctgctgctgg
     3301 cgcgaaacta catcctcatg ctcaccaact cgctggagga gatgaagcga ctggtgagcg
     3361 agatctacgg gggtcaccac gccggcttcc acccatcggc ctgtggcggg ctggcacact
     3421 cggcgcctct acccaccgcc acggcgcacc ccgcggccgc cgcgcacgcc gcgcaccacc
     3481 cggccgtgca ccaccccatc ctccctcccg cagccgccgc cgccgccgca gccgccgccg
     3541 ccgcagcggt gtccagcgcc tctctaccgg gctccgggct gtcctcggtc ggatccatcc
     3601 ggcctccgca cggcctgctc aagtcaccgt cggcagccgc agccgcccct ctcggaggcg
     3661 ggggtggagg cagcggtggc agcggtggct tccagcactg gggcggcatg ccgtgcccct
     3721 gcagcatgtg ccaggtgccg ccgccgcacc accacgtgtc ggctatgggc gccggcacct
     3781 tgccgcgcct cacctccgac gccaagtgag aattcgatat caagcttatc gataatcaac
     3841 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3901 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3961 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4021 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4081 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4141 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4201 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4261 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4321 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4381 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     4441 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     4501 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     4561 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     4621 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     4681 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     4741 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     4801 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     4861 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     4921 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     4981 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     5041 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     5101 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     5161 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     5221 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     5281 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     5341 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
     5401 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
     5461 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     5521 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     5581 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     5641 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     5701 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     5761 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     5821 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
     5881 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5941 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     6001 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     6061 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     6121 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     6181 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     6241 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     6301 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     6361 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     6421 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     6481 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     6541 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     6601 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     6661 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     6721 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6781 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     6841 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6901 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     6961 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7021 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7081 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7141 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7201 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7261 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7321 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7381 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7441 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7501 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7561 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7621 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7681 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7741 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7801 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7861 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7921 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7981 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8041 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9121 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     9181 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     9241 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     9301 accgacaatt gcatgaagaa tctgctt
//
