LOCUS       sequence-205866-       12147 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  APRT c.407T>C (APRT*J) donor vector with unilateral microhomology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, 
            Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K
  TITLE     Microhomology-assisted scarless genome editing in human iPSCs.
  JOURNAL   Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
  PUBMED    29507284
REFERENCE   2  (bases 1 to 12147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-5"; volume: "9(1):939. doi"; pages: " 
            10.1038/s41467-018-03044-y"
COMMENT     Sequence Label: pbG-APRT-J-u32uni-PdTKmCh
FEATURES             Location/Qualifiers
     source          1..12147
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..44
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(50..52,53..55,56..766)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(50..52,53..55,56..766)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(95..116)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(356..375)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(828..852)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(867..883)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(868..884)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    905..1129
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(1185..1203)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1224..1240)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1224..1240)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1237..1259)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1248..1264
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1272..1278,1279..1296,1297..1302))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1317..1338
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1455..1472)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1626..2214)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1706..1725)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2385..3176,3177..3245))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3008..3027
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3246..3350)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3376..3831)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3513..3534)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3725..3744
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3958..3980
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3972..3989
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3973..3989
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3996..4015
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3996..4014
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4839..4892
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4902..5498
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4902..4921)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5398..5418
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             5505..6500
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    6538..6762
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        6781..7160
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7162..7439
                     /label=chicken beta-actin promoter
     intron          7440..8456
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     protein_bind    8550..8574
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8580..9290
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8725..8743)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8993..9012
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     9187..9210
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(9292..9316)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(9416..9435)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9481..9536
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9535..9554)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     enhancer        10463..10842
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10844..11119
                     /label=chicken beta-actin promoter
ORIGIN
        1 gctgcaggaa ttcgatatca caagtttgta caaaaaagca ggctggacca tggtgagcaa
       61 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      121 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      181 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      241 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
      301 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      361 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      421 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      481 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
      541 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
      601 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
      661 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      721 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggcg gtggaagatc
      781 tgggaattca aggcctctcg agcctctaga ttctgcagcc ctatagcacc cagctttctt
      841 gtacaaagtg gtgatatcaa gcttatcgat accgtcgacc tcgagggccg ctgatcagcc
      901 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
      961 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     1021 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     1081 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     1141 gaaagaacca gctggggctt aattaagagc tccagctttt gttcccttta gtgagggtta
     1201 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     1261 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     1321 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     1381 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     1441 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1501 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1561 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1621 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1681 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1741 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1801 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1861 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1921 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1981 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2041 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2101 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2161 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2221 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     2281 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     2341 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     2401 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     2461 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2521 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2581 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2641 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2701 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2761 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2821 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2881 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2941 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3001 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3061 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3121 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3181 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3241 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     3301 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     3361 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     3421 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3481 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3541 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3601 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3661 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3721 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3781 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     3841 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     3901 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     3961 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
     4021 tgggtaccac tcctgtcact taccctgaca ggcctagact cccgaggctt cctctttggc
     4081 ccctccctgg cccaggagct tggactgggc tgcgtgctca tccgaaagcg ggggaagctg
     4141 ccaggcccca ctctgtgggc ctcctattcc ctggagtacg ggaaggtaag agggctgggg
     4201 tggccagagg aagggcaggg ccaggccacc gtggccactc tcccccagtt ctaaaaggcc
     4261 ttcccaggcg tgtcaagtgg agctgctgtg gttacagtgg ccttgggagc tcagagaggt
     4321 tgagacatag gctgggctca cacagccagg taacagcaag gtggggttgg agtcagggtc
     4381 tagggtggca gctgccaagc tgtgcaacaa agctgttttc tgcgggaggc tgaggaccac
     4441 acaccacttc ccactccagg ctgagctgga gattcagaaa gacgccctgg agccaggaca
     4501 gagggtggtc gtcgtggatg atctgctggc cactggtggt aagggtctcc ccgcagccaa
     4561 ctgctgtggc tccaagggcc tggtgggagt gggacaggac ctcgctgtgt gacatgggat
     4621 gcagcttact gttgtccaga gggtgcctgg tggccaggcc gacaccttcc tctccccatg
     4681 ccttcccctc cccaacccag gggctggcct ggagcacctg ctctctgcag cccaggccaa
     4741 ctggggacct caccctccca tccccaggta ccacgaacgc tgcctgtgag ctgctgggct
     4801 ccaccggcaa gctgcccgtg cccagatctg gcagcggaga gggcagagga agtcttctaa
     4861 catgcggtga cgtggaggag aatcccggcc ctaggctcga gatgaccgag tacaagccca
     4921 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     4981 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     5041 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5101 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5161 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     5221 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     5281 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     5341 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     5401 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     5461 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atccatgccc acgctactgc
     5521 gggtttatat agacggtccc cacgggatgg ggaaaaccac caccacgcaa ctgctggtgg
     5581 ccctgggttc gcgcgacgat atcgtctacg tacccgagcc gatgacttac tggcgggtgc
     5641 tgggggcttc cgagacaatc gcgaacatct acaccacaca acaccgcctc gaccagggtg
     5701 agatatcggc cggggacgcg gcggtggtaa tgacaagcgc ccagataaca atgggcatgc
     5761 cttatgccgt gaccgacgcc gttctggctc ctcatatcgg gggggaggct gggagctcac
     5821 atgccccgcc cccggccctc accctcatct tcgaccgcca tcccatcgcc gccctcctgt
     5881 gctacccggc cgcgcggtac cttatgggca gcatgacccc ccaggccgtg ctggcgttcg
     5941 tggccctcat cccgccgacc ttgcccggca ccaacatcgt gcttggggcc cttccggagg
     6001 acagacacat cgaccgcctg gccaaacgcc agcgccccgg cgagcggctg gacctggcta
     6061 tgctggctgc gattcgccgc gtttacgggc tacttgccaa tacggtgcgg tatctgcagt
     6121 gcggcgggtc gtggcgggag gactggggac agctttcggg gacggccgtg ccgccccagg
     6181 gtgccgagcc ccagagcaac gcgggcccac gaccccatat cggggacacg ttatttaccc
     6241 tgtttcgggc ccccgagttg ctggccccca acggcgacct gtataacgtg tttgcctggg
     6301 ccttggacgt cttggccaaa cgcctccgtt ccatgcacgt ctttatcctg gattacgacc
     6361 aatcgcccgc cggctgccgg gacgccctgc tgcaacttac ctccgggatg gtccagaccc
     6421 acgtcaccac ccccggctcc ataccgacga tatgcgacct ggcgcgcacg tttgcccggg
     6481 agatggggga ggctaactga tctagagggc ccgtttaaac ccgctgatca gcctcgactg
     6541 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     6601 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     6661 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     6721 aagacaatag caggcatgct ggggatgcgg tgggctctat ggactagcgg tggcggcctc
     6781 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     6841 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     6901 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     6961 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7021 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7081 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7141 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     7201 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcgr
     7261 kgggggsggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
     7321 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
     7381 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
     7441 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
     7501 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
     7561 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
     7621 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
     7681 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
     7741 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     7801 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     7861 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     7921 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     7981 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     8041 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     8101 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     8161 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     8221 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     8281 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     8341 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     8401 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     8461 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc gcggccgcgg
     8521 taccggcgcg ccggatcctg caggttatca caagtttgta caaaaaagca ggctccacca
     8581 tggtgagcaa gggcgaggag gacaacatgg ccatcatcaa ggagttcatg cgcttcaagg
     8641 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     8701 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
     8761 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     8821 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     8881 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     8941 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     9001 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     9061 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgccg
     9121 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     9181 acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
     9241 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa gacccagctt
     9301 tcttgtacaa agtggtgata actctagaga attcactcct caggtgcagg ctgcctatca
     9361 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atcgatcttt
     9421 ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct
     9481 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     9541 gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt
     9601 tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc
     9661 agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag
     9721 gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt
     9781 ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg
     9841 tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttgggcac gggcagcttg
     9901 ccggtggagt accatgaacg ctgcctgtga gctgctgggc cgcctgcagg ctgaggtcct
     9961 ggagtgcgtg agcctggtgg agctgacctc gcttaagggc agggagaagc tggcacctgt
    10021 acccttcttc tctctcctgc agtatgagtg accacagggc ctcccagccc aacatctcca
    10081 gctggatccc agggaaatat cagccttggg caactgcagt gaccaggggc accggctgcc
    10141 cacagggaac acattccttt gctggggttc agcgcctctc ctggggctgg aagtgccaaa
    10201 gcctggggca aagctgtgtt tcagccacac tgaacccaat tacacacagc gggagaacgc
    10261 agtaaacagc tttcccacaa gagccgtctc ctgtcctcct gttccccagg gcagggagcc
    10321 ccaggacaac accagacttc agctgtactg tgggcatgtg ctgcttggcg tgatgcccag
    10381 cagaacctgt cctggttctc acctcaggag ctagaaccct ccagggtacc ggccatgatg
    10441 gccgaattaa ttcaagctag tcgacattga ttattgacta gttattaata gtaatcaatt
    10501 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
    10561 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
    10621 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
    10681 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
    10741 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
    10801 acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg
    10861 ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt
    10921 ttttaattat tttgtgcagc gatgggggcg gggggggggg gggcgcgcgc caggcggggc
    10981 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
    11041 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
    11101 gcgaagcgcg cggcgggcgg gagtcgctgc gcgckgcctt cgccccgtgc cccgctccgc
    11161 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
    11221 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
    11281 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggtg
    11341 ttkkktsggg gggvbggggg ggggvggggs ggggagrggg rggggggggg gggggggggg
    11401 gggggggggg gggggggggg gggggggggg gggggggggg gggggggggg ggaasagggg
    11461 gggggggggg ggkgkggggg ggggggggkg agcrgggggt gtgggcgcgt cggtcgggct
    11521 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
    11581 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg
    11641 ggtgccgggc ggggcggggc cgcctcgggc cggggagggc tcgggggagg ggcgcggcgg
    11701 cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc ttttatggta
    11761 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
    11821 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
    11881 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
    11941 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
    12001 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
    12061 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
    12121 agaattggat cctagtggat cccccgg
//
