LOCUS       sequence-205867-        2006 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  APRT c.407T>C (APRT*J) donor vector with unilateral microhomology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Kim SI, Matsumoto T, Kagawa H, Nakamura M, Hirohata R, Ueno A, 
            Ohishi M, Sakuma T, Soga T, Yamamoto T, Woltjen K
  TITLE     Microhomology-assisted scarless genome editing in human iPSCs.
  JOURNAL   Nat Commun. 2018 Mar 5;9(1):939. doi: 10.1038/s41467-018-03044-y.
  PUBMED    29507284
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-03-5"; volume: "9(1):939. doi"; pages: " 
            10.1038/s41467-018-03044-y"
COMMENT     Sequence Label: pbG-APRT-J-u32uni-PdTKmCh
FEATURES             Location/Qualifiers
     source          1..2006
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..27
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             124..834
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(269..287)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     537..556
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     731..754
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    complement(836..860)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(960..979)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1025..1080
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1079..1098)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgcggcc
       61 gcggtaccgg cgcgccggat cctgcaggtt atcacaagtt tgtacaaaaa agcaggctcc
      121 accatggtga gcaagggcga ggaggacaac atggccatca tcaaggagtt catgcgcttc
      181 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      241 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
      301 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
      361 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
      421 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
      481 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
      541 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
      601 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
      661 gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
      721 gtcaacatca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
      781 gagcgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagaccca
      841 gctttcttgt acaaagtggt gataactcta gagaattcac tcctcaggtg caggctgcct
      901 atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgat
      961 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     1021 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     1081 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     1141 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     1201 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     1261 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     1321 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     1381 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg gcacgggcag
     1441 cttgccggtg gagtaccatg aacgctgcct gtgagctgct gggccgcctg caggctgagg
     1501 tcctggagtg cgtgagcctg gtggagctga cctcgcttaa gggcagggag aagctggcac
     1561 ctgtaccctt cttctctctc ctgcagtatg agtgaccaca gggcctccca gcccaacatc
     1621 tccagctgga tcccagggaa atatcagcct tgggcaactg cagtgaccag gggcaccggc
     1681 tgcccacagg gaacacattc ctttgctggg gttcagcgcc tctcctgggg ctggaagtgc
     1741 caaagcctgg ggcaaagctg tgtttcagcc acactgaacc caattacaca cagcgggaga
     1801 acgcagtaaa cagctttccc acaagagccg tctcctgtcc tcctgttccc cagggcaggg
     1861 agccccagga caacaccaga cttcagctgt actgtgggca tgtgctgctt ggcgtgatgc
     1921 ccagcagaac ctgtcctggt tctcacctca ggagctagaa ccctccaggg taccggccat
     1981 gatggccgaa ttaattcaag ctagtc
//
