LOCUS       sequence-205879-        4783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human full-length LSD1 to be expressed in a 
            baculovirus/insect cell expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Inui K, Zhao Z, Yuan J, Jayaprakash S, Le LTM, Drakulic S, Sander B,
            Golas MM
  TITLE     Stepwise assembly of functional C-terminal REST/NRSF transcriptional
            repressor complexes as a drug target.
  JOURNAL   Protein Sci. 2017 May;26(5):997-1011. doi: 10.1002/pro.3142. Epub 
            2017 Mar 12.
  PUBMED    28218430
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3142"; 
            journalName: "Protein Sci"; date: "2017-05"; volume: "26"; issue: 
            "5"; pages: "997-1011"
COMMENT     Sequence Label: pIDS-LSD1fl
FEATURES             Location/Qualifiers
     source          1..4783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..125
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     primer_bind     complement(2769..2788)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2813..2861
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    3063..3096
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      3098..3454
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             3917..4708
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgatactagt atacggacct ttaattcaac ccaacacaat atattatagt taaataagaa
       61 ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac attttattta
      121 caatcactcg acgaagactt gatcacccgg gatctcgaga tgttgtccgg aaagaaggcc
      181 gccgctgccg ccgccgccgc tgccgctgct gccacaggta ccgaagctgg tcctggaacc
      241 gccggaggtt ctgaaaacgg atccgaagtc gctgcccaac ccgctggtct gtcaggtccc
      301 gccgaagttg gacctggagc tgtgggcgaa cgtactccaa ggaaaaagga accccctcgt
      361 gcctcccctc ccggtggact ggctgagcca cctggatccg ccggacctca ggctggtccc
      421 acagtcgtgc ccggttccgc tacacccatg gagacaggta tcgctgaaac ccctgaaggt
      481 cgtcgtacct cacgtcgtaa gcgtgccaag gtggaataca gagaaatgga tgagagtctg
      541 gctaacttgt cagaggatga gtactactct gaagaggaac gcaacgctaa ggccgagaag
      601 gagaaaaagt tgcctccccc tccaccacag gctcctccag aggaggaaaa cgaatcagaa
      661 cccgaagagc caagcggtgt cgagggcgct gctttccaaa gccgtttgcc tcatgatcgt
      721 atgacatccc aggaggccgc ctgcttccct gacatcatca gcggccccca acagacacag
      781 aaagttttcc tcttcatcag gaacagaacc ctccaactgt ggctcgacaa cccaaagatt
      841 cagctcactt tcgaggctac actgcaacaa ctcgaagctc cttataactc tgatactgtg
      901 ctggtccaca gagtccactc ttacctcgaa cgccatggtc tgatcaactt cggaatctac
      961 aagcgcatta agcccctgcc taccaaaaag actggtaaag tgatcatcat cggttcaggc
     1021 gttagcggcc tggctgctgc ccgccagctc caaagtttcg gtatggacgt caccctgctg
     1081 gaggctaggg accgcgtcgg aggcagggtt gctacattca ggaagggcaa ctacgttgcc
     1141 gacctcggcg ccatggttgt taccggcctg ggtggtaacc ccatggctgt ggtgagcaag
     1201 caggtcaaca tggaactcgc caagattaag caaaagtgcc ctctctacga agctaatggc
     1261 caggccgtgc caaaggaaaa ggacgaaatg gtggaacagg agttcaaccg cctgctggag
     1321 gccactagct acctctccca ccagctggac ttcaacgtgc tgaataacaa acctgttagt
     1381 ctgggtcaag ccctggaagt ggttatccag ctgcaagaga agcacgttaa ggacgagcag
     1441 atcgaacact ggaagaagat cgttaagact caagaagagc tgaaagaact cctcaacaag
     1501 atggttaacc tgaaggaaaa aatcaaggag ctgcaccaac aatacaagga ggcctcagaa
     1561 gttaagccac ccagagacat cactgctgag ttcctggtca agtctaagca cagggatctg
     1621 accgctctgt gcaaggaata cgacgaactg gctgagactc agggcaagct ggaggagaag
     1681 ctccaggagt tggaggccaa cccacccagt gacgtttacc tgtcttcccg tgaccgccag
     1741 atcctggatt ggcacttcgc caacttggag tttgctaatg ccaccccatt gagtaccctc
     1801 tctctcaagc actgggacca agatgatgac ttcgagttca ccggcagcca cttgactgtg
     1861 cgcaacggtt actcatgtgt tccagtcgcc ttggccgagg gcctcgacat taagttgaat
     1921 acagctgtca ggcaagtgag gtacactgct tctggctgtg aggtcatcgc tgttaacaca
     1981 cgcagcacat cccagacatt tatttacaaa tgtgacgctg tcctctgtac cttgccactg
     2041 ggcgtgctca aacagcaacc ccccgctgtg cagttcgtgc cccctctccc agagtggaag
     2101 accagcgccg tccagcgcat gggtttcgga aacctgaata aggtggtctt gtgctttgat
     2161 agggttttct gggatccttc cgtcaacctg ttcggccatg tgggttctac tactgcctct
     2221 cgcggagaac tgttcttgtt ttggaacctc tacaaagctc caattctctt ggccctcgtt
     2281 gccggcgagg ccgccggaat catggaaaac atctccgacg acgtgattgt tggccgttgt
     2341 ttggccatcc tgaagggcat cttcggatca tccgccgtgc cccaacctaa ggaaactgtg
     2401 gtgagccgct ggcgtgctga cccatgggct cgtggcagct actcatacgt cgctgctgga
     2461 tcttccggca atgactatga cctgatggct cagccaatca caccaggtcc tagcatccct
     2521 ggcgcccctc agcccatccc ccgcctcttt ttcgccggag aacacacaat ccgtaactac
     2581 ccagccacag tgcacggagc tctcctgtca ggactccgcg aggccggtcg tatcgccgac
     2641 cagttcctcg gcgccatgta cactctgcca cgccaagcca ctccaggtgt ccccgcccag
     2701 caaagccctt caatgtaagc tagcagctga tgcatagcat gcggtaccgg gagatggggg
     2761 aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata
     2821 aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     2881 ccagggctgg cactctgtcg ataccccacc gagaccccat tgggaccaat acgcccgcgt
     2941 ttcttccttt tccccacccc aacccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     3001 cgtcggggcg gcaagccctg ccatagccac tacgggtacg tttaaaccca tgtgcctggc
     3061 agataacttc gtataatgta tgctatacga agttatggta cgtactaagc tctcatgttt
     3121 cacgtactaa gctctcatgt ttaacgtact aagctctcat gtttaacgaa ctaaaccctc
     3181 atggctaacg tactaagctc tcatggctaa cgtactaagc tctcatgttt cacgtactaa
     3241 gctctcatgt ttgaacaata aaattaatat aaatcagcaa cttaaatagc ctctaaggtt
     3301 ttaagtttta taagaaaaaa aagaatatat aaggctttta aagcttttaa ggtttaacgg
     3361 ttgtggacaa caagccaggg atgtaacgca ctgagaagcc cttagagcct ctcaaagcaa
     3421 ttttgagtga cacaggaaca cttaacggct gacataattc agcttcacgc tgccgcaagc
     3481 actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt ccgcagaaac
     3541 ggtgctgacc ccggatgaat gtcagctggg aggcagaata aatgatcata tcgtcaatta
     3601 ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca
     3661 cactgcttcc ggtagtcaat aaaccggtaa gtagcgtatg cgctcacgca actggtccag
     3721 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     3781 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     3841 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     3901 aacaaagtta aacatcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     3961 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     4021 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     4081 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     4141 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     4201 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     4261 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     4321 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     4381 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     4441 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     4501 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     4561 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     4621 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     4681 cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacggatcta
     4741 tgtcgggtgc ggagaaagag gtaatgaaat ggcacctagg tat
//
