LOCUS       sequence-20590-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG304GPD-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(38..162)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(170..186)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(170..186)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(192..455)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(275..298)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 taaagtgtaa agcctggggg tggcctaatg cggccgccat agtgactgga tatgttgtgt
       61 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
      121 atcattttac gtttctcgtt cagctttttt gtacaaactt gtgatggggg atccactagt
      181 tctagaatcc gtcgaaacta agttctggtg ttttaaaact aaaaaaaaga ctaactataa
      241 aagtagaatt taagaagttt aagaaataga tttacagaat tacaatcaat acctaccgtc
      301 tttatatact tattagtcaa gtaggggaat aatttcaggg aactggtttc aacctttttt
      361 ttcagctttt tccaaatcag agagagcaga aggtaataga aggtgtaaga aaatgagata
      421 gatacatgcg tgggtcaatt gccttgtgtc atcatttact ccaggcaggt tgcatcactc
      481 cattgaggtt gtgcccgttt tttgcctgtt tgtgcccctg ttctctgtag ttgcgctaag
      541 agaatggacc tatgaactga tggttggtga agaaaacaat attttggtgc tgggattctt
      601 tttttttctg gatgccagct taaaaagcgg gctccattat atttagtgga tgccaggaat
      661 aaactgttca cccagacacc tacgatgtta tatattctgt gtaacccgcc ccctattttg
      721 ggcatgtacg
//
