LOCUS       sequence-205912-        2461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human full-length FCER1G to be expressed in a 
            baculovirus/insect cell expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Le LTM, Nyengaard JR, Golas MM, Sander B
  TITLE     Vectors for Expression of Signal Peptide-Dependent Proteins in 
            Baculovirus/Insect Cell Systems and Their Application to Expression 
            and Purification of the High-Affinity Immunoglobulin Gamma Fc 
            Receptor I in Complex with Its Gamma Chain.
  JOURNAL   Mol Biotechnol. 2018 Jan;60(1):31-40. doi: 
            10.1007/s12033-017-0041-8.
  PUBMED    29143175
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biotechnol"; date: "2018-01"; volume: "60"; issue: "1"; pages: 
            "31-40"
COMMENT     Sequence Label: pGS-FCER1G-1
FEATURES             Location/Qualifiers
     source          1..2461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..125
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     primer_bind     complement(447..466)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    491..539
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    741..774
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      776..1132
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             1595..2386
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cgatactagt atacggacct ttaattcaac ccaacacaat atattatagt taaataagaa
       61 ttattatcaa atcatttgta tattaattaa aatactatac tgtaaattac attttattta
      121 caatcactcg acgaagactt gatcacccgg gatctcgaga tgatccccgc tgtggttctt
      181 ctgcttctcc tcttggttga gcaggctgct gctctcggag aaccacaact ctgttacatc
      241 ctggacgcga tcttgtttct gtacggtatc gtgctcaccc tgctgtactg tcgtcttaaa
      301 attcaagttc gcaaggcagc cattaccagt tatgagaaaa gcgacggtgt ctataccggc
      361 ctctccacgc gtaaccagga gacttacgag actctgaaac atgaaaagcc cccccaataa
      421 ggtaccggga gatgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc
      481 cgcgctatga cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca
      541 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
      601 ggaccaatac gcccgcgttt cttccttttc cccaccccaa cccccaagtt cgggtgaagg
      661 cccagggctc gcagccaacg tcggggcggc aagccctgcc atagccacta cgggtacgtt
      721 taaacccatg tgcctggcag ataacttcgt ataatgtatg ctatacgaag ttatggtacg
      781 tactaagctc tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt
      841 ttaacgaact aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc
      901 tcatgtttca cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact
      961 taaatagcct ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa
     1021 gcttttaagg tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct
     1081 tagagcctct caaagcaatt ttgagtgaca caggaacact taacggctga cataattcag
     1141 cttcacgctg ccgcaagcac tcagggcgca agggctgcta aaggaagcgg aacacgtaga
     1201 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctgggag gcagaataaa
     1261 tgatcatatc gtcaattatt acctccacgg ggagagcctg agcaaactgg cctcaggcat
     1321 ttgagaagca cacggtcaca ctgcttccgg tagtcaataa accggtaagt agcgtatgcg
     1381 ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt
     1441 tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca
     1501 gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca
     1561 gcagggcagt cgccctaaaa caaagttaaa catcatgagg gaagcggtga tcgccgaagt
     1621 atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct
     1681 ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga
     1741 tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga
     1801 ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac
     1861 cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt
     1921 tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat
     1981 tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc
     2041 ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac
     2101 cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac
     2161 gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc
     2221 cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca
     2281 ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt
     2341 tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc taacaattcg
     2401 ttcaagccga cggatctatg tcgggtgcgg agaaagaggt aatgaaatgg cacctaggta
     2461 t
//
