LOCUS       sequence-20594-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG305GAL-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(117..241)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(318..339)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(579..598)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
       61 gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata
      121 gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
      181 ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg
      241 tgatgggctg caggaattcg atatcaagct taatggtgag caagggcgag gagctgttca
      301 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      361 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      421 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctgc
      481 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      541 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      601 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aaaggcatcg
      661 acttcaagga ggacggcaac atcctg
//
