LOCUS       sequence-205958-        9420 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Scott KL, Kabbarah O, Liang MC, Ivanova E, Anagnostou V, Wu J, 
            Dhakal S, Wu M, Chen S, Feinberg T, Huang J, Saci A, Widlund HR, 
            Fisher DE, Xiao Y, Rimm DL, Protopopov A, Wong KK, Chin L
  TITLE     GOLPH3 modulates mTOR signalling and rapamycin sensitivity in 
            cancer.
  JOURNAL   Nature. 2009 Jun 25. 459(7250):1085-90.
  PUBMED    19553991
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Jun 25. 459(7250):1085-90."
COMMENT     Sequence Label: pLenti6/V5/DEST-VPS35
FEATURES             Location/Qualifiers
     source          1..9420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      54..642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     543..562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     796..813
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    930..951
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(966..971,972..989,990..996)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1004..1020
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1009..1031
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1028..1044
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1028..1044
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1063..1083
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1065..1083
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1111..1337
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1338..1518
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1565..1690
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2183..2416
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2601..2645
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2924..3227
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3228..3431
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3382..3402
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3428..3452
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    3548..3572
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5961..5985)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6038..6079
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6116..6136)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6121..6450
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6301..6436
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6498..6545
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6564..6962
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7049..7282
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7354..7475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7391..7410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7445..7464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7515..7650
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7670..7689)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7671..7689)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7699..7716)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7699..7715)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7708..7730)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7857..8312
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7944..7963)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8154..8175
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8338..8442
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8443..8511,8512..9303)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8661..8680)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
       61 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
      121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
      181 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
      241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
      301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
      361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      421 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
      661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
      721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
      781 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
      841 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
      901 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      961 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     1021 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     1081 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     1141 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     1201 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     1261 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     1321 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     1381 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     1441 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     1501 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     1561 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     1621 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     1681 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     1741 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     1801 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     1861 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     1921 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     1981 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     2041 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     2101 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     2161 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     2221 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     2281 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     2341 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     2401 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     2461 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     2521 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     2581 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     2641 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     2701 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     2761 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
     2821 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
     2881 tagtgaacgg atctcgacgg tatcgataag cttgggagtt ccgcgttaca taacttacgg
     2941 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     3001 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     3061 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     3121 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     3181 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     3241 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     3301 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     3361 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     3421 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     3481 acctccatag aagacaccga ctctagagga tccactagtc cagtgtggtg gaattctgca
     3541 gatatcaaca agtttgtaca aaaaagttgg catgcctaca acacagcagt cccctcagga
     3601 tgagcaggaa aagctcttgg atgaagccat acaggctgtg aaggtccagt cattccaaat
     3661 gaagagatgc ctggacaaaa acaagcttat ggatgctcta aaacatgctt ctaatatgct
     3721 tggtgaactc cggacttcta tgttatcacc aaagagttac tatgaacttt atatggccat
     3781 ttctgatgaa ctgcactact tggaggtcta cctgacagat gagtttgcta aaggaaggaa
     3841 agtggcagat ctctacgaac ttgtacagta tgctggaaac attatcccaa ggctttacct
     3901 tttgatcaca gttggagttg tatatgtcaa gtcatttcct cagtccagga aggatatttt
     3961 gaaagatttg gtagaaatgt gccgtggtgt gcaacatccc ttgaggggtc tgtttcttcg
     4021 aaattacctt cttcagtgta ccagaaatat cttacctgat gaaggagagc caacagatga
     4081 agaaacaact ggtgacatca gtgattccat ggattttgta ctgctcaact ttgcagaaat
     4141 gaacaagctc tgggtgcgaa tgcagcatca gggacatagc cgagatagag aaaaaagaga
     4201 acgagaaaga caagaactga gaattttagt gggaacaaat ttggtgcgcc tcagtcagtt
     4261 ggaaggtgta aatgtggaac gttacaaaca gattgttttg actggcatat tggagcaagt
     4321 tgtaaactgt agggatgctt tggctcaaga atatctcatg gagtgtatta ttcaggtttt
     4381 ccctgatgaa tttcacctcc agactttgaa tccttttctt cgggcctgtg ctgagttaca
     4441 ccagaatgta aatgtgaaga acataatcat tgctttaatt gatagattag ctttatttgc
     4501 tcaccgtgaa gatggacctg gaatcccagc ggatattaaa ctttttgata tattttcaca
     4561 gcaggtggct acagtgatac agtctagaca agacatgcct tcagaggatg ttgtatcttt
     4621 acaagtctct ctgattaatc ttgccatgaa atgttaccct gatcgtgtgg actatgttga
     4681 taaagttcta gaaacaacag tggagatatt caataagctc aaccttgaac atattgctac
     4741 cagtagtgca gtttcaaagg aactcaccag acttttgaaa ataccagttg acacttacaa
     4801 caatatttta acagtcttga aattaaaaca ttttcaccca ctctttgagt actttgacta
     4861 cgagtccaga aagagcatga gttgttatgt gcttagtaat gttctggatt ataacacaga
     4921 aattgtctct caagaccagg tggattccat aatgaatttg gtatccacgt tgattcaaga
     4981 tcagccagat caacctgtag aagaccctga tccagaagat tttgctgatg agcagagcct
     5041 tgtgggccgc ttcattcatc tgctgcgctc tgaggaccct gaccagcagt acttgatttt
     5101 gaacacagca cgaaaacatt ttggagctgg tggaaatcag cggattcgct tcacactgcc
     5161 acctttggta tttgcagctt accagctggc ttttcgatat aaagagaatt ctaaagtgga
     5221 tgacaaatgg gaaaagaaat gccagaagat tttttcattt gcccaccaga ctatcagtgc
     5281 tttgatcaaa gcagagctgg cagaattgcc cttaagactt tttcttcaag gagcactagc
     5341 tgctggggaa attggttttg aaaatcatga gacagtcgca tatgaattca tgtcccaggc
     5401 attttctctg tatgaagatg aaatcagcga ttccaaagca cagctagctg ccatcacctt
     5461 gatcattggc acttttgaaa ggatgaagtg cttcagtgaa gagaatcatg aacctctgag
     5521 gactcagtgt gcccttgctg catccaaact tctaaagaaa cctgatcagg gccgagctgt
     5581 gagcacctgt gcacatctct tctggtctgg cagaaacacg gacaaaaatg gggaggagct
     5641 tcacggaggc aagagggtaa tggagtgcct aaaaaaagct ctaaaaatag caaatcagtg
     5701 catggacccc tctctacaag tgcagctttt tatagaaatt ctgaacagat atatctattt
     5761 ttatgaaaag gaaaatgatg cggtaacaat tcaggtttta aaccagctta tccaaaagat
     5821 tcgagaagac ctcccgaatc ttgaatccag tgaagaaaca gagcagatta acaaacattt
     5881 tcataacaca ctggagcatt tgcgcttgcg gcgggaatca ccagaatccg aggggccaat
     5941 ttatgaaggt ctcatccttt actcaacttt cttgtacaaa gtggttgata tccagcacag
     6001 tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc ctaaccctct
     6061 cctcggtctc gattctacgc gtaccggtta gtaatgagtt tggaattaat tctgtggaat
     6121 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6181 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     6241 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6301 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6361 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     6421 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     6481 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     6541 acgacaaggt gaggaactaa accatggcca agcctttgtc tcaagaagaa tccaccctca
     6601 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     6661 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     6721 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     6781 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     6841 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     6901 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     6961 aagcacaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7021 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7081 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     7141 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     7201 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     7261 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     7321 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7381 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7441 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     7501 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7561 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7621 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     7681 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     7741 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     7801 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     7861 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     7921 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     7981 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8041 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8101 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8161 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8221 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8281 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     8341 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8401 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8461 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8521 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8581 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     8641 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     8701 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     8761 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     8821 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     8881 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     8941 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9001 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9061 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9121 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9181 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9241 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9301 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9361 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
//
