LOCUS       sequence-205964-        5187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  contains G94A mutation in pSOD1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Stevens JC, Chia R, Hendriks WT, Bros-Facer V, van Minnen J, Martin 
            JE, Jackson GS, Greensmith L, Schiavo G, Fisher EM
  TITLE     Modification of superoxide dismutase 1 (SOD1) properties by a GFP 
            tag--implications for research into amyotrophic lateral sclerosis 
            (ALS).
  JOURNAL   PLoS One. 2010 . 5(3):e9541.
  PUBMED    20221404
REFERENCE   2  (bases 1 to 5187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010 . 5(3):e9541."
COMMENT     Sequence Label: pF150 pSOD1G93AAcGFP1
FEATURES             Location/Qualifiers
     source          1..5187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      21..609
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     510..529
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        784..1087
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1088..1291
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1242..1262
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1857..2576
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     polyA_signal    2698..2819
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2735..2754)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2789..2808
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2826..3281)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2963..2984)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3175..3194
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3308..3412
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3414..3771
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3414..3605
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3437..3457)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3622..3757
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3806..4600
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3806..4600
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3860..3879)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4470..4489
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4788..4807)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4832..4879
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
       61 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
      121 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
      181 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
      241 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
      301 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
      361 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
      421 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
      481 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
      541 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
      601 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
      661 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg
      721 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      781 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      841 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      901 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      961 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     1021 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     1081 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     1141 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     1201 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     1261 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
     1321 ctaccggact cagatctcga gctcaagctt cgaattccat ggcgacgaag gccgtgtgcg
     1381 tgctgaaggg cgacggccca gtgcagggca tcatcaattt cgagcagaag gaaagtaatg
     1441 gaccagtgaa ggtgtgggga agcattaaag gactgactga aggcctgcat ggattccatg
     1501 ttcatgagtt tggagataat acagcaggct gtaccagtgc aggtcctcac tttaatcctc
     1561 tatccagaaa acacggtggg ccaaaggatg aagagaggca tgttggagac ttgggcaatg
     1621 tgactgctga caaagatgct gtggccgatg tgtctattga agattctgtg atctcactct
     1681 caggagacca ttgcatcatt ggccgcacac tggtggtcca tgaaaaagca gatgacttgg
     1741 gcaaaggtgg aaatgaagaa agtacaaaga caggaaacgc tggaagtcgt ttggcttgtg
     1801 gtgtaattgg gatcgcccaa gggtcgacgg taccgcgggc ccgggatcca ccggtcatgg
     1861 tgagcaaggg cgccgagctg ttcaccggca tcgtgcccat cctgatcgag ctgaatggcg
     1921 atgtgaatgg ccacaagttc agcgtgagcg gcgagggcga gggcgatgcc acctacggca
     1981 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc tgtgccctgg cccaccctgg
     2041 tgaccaccct gagctacggc gtgcagtgct tctcacgcta ccccgatcac atgaagcagc
     2101 acgacttctt caagagcgcc atgcctgagg gctacatcca ggagcgcacc atcttcttcg
     2161 aggatgacgg caactacaag tcgcgcgccg aggtgaagtt cgagggcgat accctggtga
     2221 atcgcatcga gctgaccggc accgatttca aggaggatgg caacatcctg ggcaataaga
     2281 tggagtacaa ctacaacgcc cacaatgtgt acatcatgac cgacaaggcc aagaatggca
     2341 tcaaggtgaa cttcaagatc cgccacaaca tcgaggatgg cagcgtgcag ctggccgacc
     2401 actaccagca gaataccccc atcggcgatg gccctgtgct gctgcccgat aaccactacc
     2461 tgtccaccca gagcgccctg tccaaggacc ccaacgagaa gcgcgatcac atgatctact
     2521 tcggcttcgt gaccgccgcc gccatcaccc acggcatgga tgagctgtac aagtgagcgg
     2581 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2641 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2701 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2761 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     2821 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2881 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2941 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     3001 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     3061 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     3121 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     3181 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     3241 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     3301 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3361 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     3421 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3481 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     3541 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     3601 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3661 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     3721 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     3781 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     3841 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3901 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3961 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     4021 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     4081 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     4141 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     4201 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4261 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4321 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4381 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4441 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4501 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     4561 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     4621 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     4681 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     4741 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     4801 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     4861 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     4921 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     4981 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     5041 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     5101 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     5161 tcgttccact gagcgtcaga ccccgta
//
