LOCUS       sequence-205968-        5272 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5272)
  AUTHORS   Yang C, Tan W, Whittle C, Qiu L, Cao L, Akbarian S, Xu Z
  TITLE     The C-terminal TDP-43 fragments have a high aggregation propensity 
            and harm neurons by a dominant-negative mechanism.
  JOURNAL   PLoS One. 2010  . 5(12):e15878.
  PUBMED    21209826
REFERENCE   2  (bases 1 to 5272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(12):e15878."
COMMENT     Sequence Label: TDP43 NOTAG1
FEATURES             Location/Qualifiers
     source          1..5272
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     330..352
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     414..433
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(478..511)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(2305..2324)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2370..2425
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2424..2443)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(2786..2802)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2786..2802)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2799..2821)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2810..2826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2834..2840,2841..2858,2859..2864))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2879..2900
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2959..3154
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3005..3140
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3160..3294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3197..3216)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3251..3270
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3362..3379)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3533..4121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3613..3632)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4292..5083,5084..5152))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4915..4934
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5153..5257)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg
       61 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc
      121 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
      181 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc
      241 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg
      301 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc
      361 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
      421 gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgatcgag ggacctaata
      481 acttcgtata gcatacatta tacgaagtta tattaagggt tattgaatag atcggaattc
      541 ctgcagcccg ggggatccgg atcagatctc gagatgtctg aatatattcg ggtaaccgaa
      601 gatgagaacg atgagcccat tgaaatacca tcggaagacg atgggacggt gctgctctcc
      661 acggttacag cccagtttcc aggggcgtgt gggcttcgct acaggaatcc agtgtctcag
      721 tgtatgagag gtgtccggct ggtagaagga attctgcatg ccccagatgc tggctgggga
      781 aatctggtgt atgttgtcaa ctatccaaaa gataacaaaa gaaaaatgga tgagacagat
      841 gcttcatcag cagtgaaagt gaaaagagca gtccagaaaa catccgattt aatagtgttg
      901 ggtctcccat ggaaaacaac cgaacaggac ctgaaagagt attttagtac ctttggagaa
      961 gttcttatgg tgcaggtcaa gaaagatctt aagactggtc attcaaaggg gtttggcttt
     1021 gttcgtttta cggaatatga aacacaagtg aaagtaatgt cacagcgaca tatgatagat
     1081 ggacgatggt gtgactgcaa acttcctaat tctaagcaaa gccaagatga gcctttgaga
     1141 agcagaaaag tgtttgtggg gcgctgtaca gaggacatga ctgaggatga gctgcgggag
     1201 ttcttctctc agtacgggga tgtgatggat gtcttcatcc ccaagccatt cagggccttt
     1261 gcctttgtta catttgcaga tgatcagatt gcgcagtctc tttgtggaga ggacttgatc
     1321 attaaaggaa tcagcgttca tatatccaat gccgaaccta agcacaatag caatagacag
     1381 ttagaaagaa gtggaagatt tggtggtaat ccaggtggct ttgggaatca gggtggattt
     1441 ggtaatagca gagggggtgg agctggtttg ggaaacaatc aaggtagtaa tatgggtggt
     1501 gggatgaact ttggtgcgtt cagcattaat ccagccatga tggctgccgc ccaggcagca
     1561 ctacagagca gttggggtat gatgggcatg ttagccagcc agcagaacca gtcaggccca
     1621 tcgggtaata accaaaacca aggcaacatg cagagggagc caaaccaggc cttcggttct
     1681 ggaaataact cttatagtgg ctctaattct ggtgcagcaa ttggttgggg atcagcatcc
     1741 aatgcagggt cgggcagtgg ttttaatgga ggctttggct caagcatgga ttctaagtct
     1801 tctggctggg gaatgtagac gcgttcctga ccattcatcc tctttctttt tcctgcaggc
     1861 ttgtggaaga aatgggaccc tgctgctgct ggagacggcc actgctccct gagtgtcccg
     1921 tccctggggc tcctgcttcc ccatcccgga gttctcctgc tccccacctc ctgtcgtcct
     1981 gcctggcctc cagcccgagt cgtcatcccc ggagtcccta tacagaggtc ctgccatgga
     2041 accttcccct ccccgtgcgc tctggggact gagcccatga caccaaacct gccccttggc
     2101 tgctctcgga ctccctaccc caaccccagg gactgcaggt tgtgccctgt ggctgcctgc
     2161 accccaggaa aggagggggc tcaggccatc cagccaccac ctacagccca gtggggtcga
     2221 ggaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg tggccaatgc
     2281 cctggctcac aaataccact gagatctttt tccctctgcc aaaaattatg gggacatcat
     2341 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
     2401 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
     2461 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc tggctgccat
     2521 gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     2581 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     2641 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     2701 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct
     2761 gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     2821 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2881 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     2941 tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     3001 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     3061 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     3121 aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat tgcagcttat
     3181 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3241 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgctgc
     3301 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     3361 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     3421 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     3481 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     3541 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     3601 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     3661 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     3721 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     3781 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     3841 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     3901 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     3961 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4021 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     4081 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     4141 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     4201 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     4261 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4321 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4381 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4441 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4501 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     4561 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     4621 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     4681 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     4741 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     4801 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     4861 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     4921 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     4981 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5041 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5101 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5161 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5221 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aa
//
