LOCUS       sequence-205969-        6418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent protein for superresolution imaging and a focal adhesion
            protein for binding substrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Wang S, Moffitt JR, Dempsey GT, Xie XS, Zhuang X
  TITLE     Characterization and development of photoactivatable fluorescent 
            proteins for single-molecule-based superresolution imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8452-7. doi: 
            10.1073/pnas.1406593111. Epub 2014 May 27.
  PUBMED    24912163
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1406593111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-10- 10"; volume: "111"; issue: "23"; pages: "8452-7"
COMMENT     Sequence Label: pZyxin-mMaple3
FEATURES             Location/Qualifiers
     source          1..6418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        168..471
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        472..675
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     626..646
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      741..750
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2471..2480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3313..3434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3350..3369)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3404..3423
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3441..3896)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3578..3599)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3790..3809
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3923..4027
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4029..4386
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4029..4220
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4052..4072)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4237..4372
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4421..5215
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4421..5215
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4475..4494)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5085..5104
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5403..5422)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5447..5494
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5823..6411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6312..6331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
       61 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcattag ttattaatag
      121 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      181 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      241 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      301 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      361 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      421 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      481 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      541 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      601 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      661 tatataagca gagctggttt agtgaaccgt cagatccgct agcgctaccg gactcagatc
      721 tcgagctcaa gcttcgaatt ccatggcggc cccccgcccg tctcccgcga tccccgtttc
      781 ggtctcggct ccggcttttt acgccccgca gaagaagttc ggccctgtgg tggccccaaa
      841 gcccaaagtg aatcccttcc ggcccaggga cagcgagcct cccccggcac ccggggccca
      901 gcgcgcacag atgggccggg tgggcgatat tcccccgccg cccccggaag actttcccct
      961 gcctccacct ccccttgctg gggatggcga cgatgcagag ggtgctctgg gaggtgcctt
     1021 cccgccgccc cctcccccga tcgaggaatc atttccccct gcgcctctgg aggaggagat
     1081 cttcccttcc ccgccgcctc ctccggagga ggagggaggg cctgaggccc ccataccgcc
     1141 cccaccacag cccagggaga aggtgagcag tattgatttg gagatcgact ctctgtcctc
     1201 actgctggat gacatgacca agaatgatcc tttcaaagcc cgggtgtcat ctggatatgt
     1261 gcccccacca gtggccactc cattcagttc caagtccagt accaagcctg cagccggggg
     1321 cacagcaccc ctgcctcctt ggaagtcccc ttccagctcc cagcctctgc cccaggttcc
     1381 ggctccggct cagagccaga cacagttcca tgttcagccc cagccccagc ccaagcctca
     1441 ggtccaactc catgtccagt cccagaccca gcctgtgtct ttggctaaca cccagccccg
     1501 agggccccca gcctcatctc cggctccagc ccctaagttt tctccagtga ctcctaagtt
     1561 tactcctgtg gcttccaagt tcagtcctgg agccccaggt ggatctgggt cacaaccaaa
     1621 tcaaaaattg gggcaccccg aagctctttc tgctggcaca ggctcccctc aacctcccag
     1681 cttcacctat gcccagcaga gggagaagcc ccgagtgcag gagaagcagc accccgtgcc
     1741 cccaccggct cagaaccaaa accaggtgcg ctcccctggg gccccagggc ccctgactct
     1801 gaaggaggtg gaggagctgg agcagctgac ccagcagcta atgcaggaca tggagcatcc
     1861 tcagaggcag aatgtggctg tcaacgaact ctgcggccga tgccatcaac ccctggcccg
     1921 ggcgcagcca gccgtccgcg ctctagggca gctgttccac atcgcctgct tcacctgcca
     1981 ccagtgtgcg cagcagctcc agggccagca gttctacagt ctggaggggg cgccgtactg
     2041 cgagggctgt tacactgaca ccctggagaa gtgtaacacc tgcggggagc ccatcactga
     2101 ccgcatgctg agggccacgg gcaaggccta tcacccgcac tgcttcacct gtgtggtctg
     2161 cgcccgcccc ctggagggca cctccttcat cgtggaccag gccaaccggc cccactgtgt
     2221 ccccgactac cacaagcagt acgccccgag gtgctccgtc tgctctgagc ccatcatgcc
     2281 tgagcctggc cgagatgaga ctgtgcgagt ggtcgccctg gacaagaact tccacatgaa
     2341 gtgttacaag tgtgaggact gcgggaagcc cctgtcgatt gaggcagatg acaatggctg
     2401 cttccccctg gacggtcacg tgctctgtcg gaagtgccac actgctagag cccagacgga
     2461 tccaccggtc gccaccatgg tgagcaaagg cgaggagaca atcatgtccg tgatcaagcc
     2521 cgacatgaag atcaaactga ggatggaggg caacgtgaac ggccacgcct tcgtgatcga
     2581 gggcgaagga agcggcaagc ccttcgaggg catccagacc atcgatctgg aggtcaagga
     2641 gggcgctccc ctccctttcg cctatgacat cctgaccacc gccttccact acggcaatag
     2701 ggtgttcacc aagtatccca ggaagatccc cgactacttc aagcagagct tccctgaggg
     2761 ctacagctgg gagaggagca tgacatacga ggacggcggc atctgcaacg ccaccaacga
     2821 catcacaatg gaggaggaca gcttcatcaa caagatccac ttcaaaggca caaacttccc
     2881 ccccaatggc cccgtgatgc agaagaggac cgtgggctgg gaggtgagca ccgagaagat
     2941 gtacgtgagg gacggcgtcc tgaagggcga cgtgaagatg aagctcctgc tcaagggcgg
     3001 cagccactac aggtgcgact ttaggaccac ctataaggtg aagcagaagg ctgtgaagct
     3061 gcccaaggcc cacttcgtcg accataggat cgagatcctg tcccacgaca aggactacaa
     3121 caaggtcaag ctgtacgagc acgccgtcgc taggaacagc accgacagca tggacgaact
     3181 ctataagtga agcggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt
     3241 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     3301 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3361 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3421 atcaatgtat cttaaggcgt aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa
     3481 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa
     3541 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact
     3601 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc
     3661 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa
     3721 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc
     3781 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt
     3841 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg
     3901 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3961 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4021 gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     4081 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4141 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     4201 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4261 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4321 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     4381 tgcaaagatc gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     4441 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     4501 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     4561 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     4621 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     4681 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     4741 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     4801 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     4861 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     4921 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     4981 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     5041 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     5101 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     5161 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg
     5221 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat
     5281 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     5341 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccctag ggggaggcta
     5401 actgaaacac ggaaggagac aataccggaa ggaacccgcg ctatgacggc aataaaaaga
     5461 cagaataaaa cgcacggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     5521 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     5581 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     5641 gcggcaggcc ctgccatagc ctcaggttac tcatatatac tttagattga tttaaaactt
     5701 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5761 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5821 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5881 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5941 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     6001 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6061 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6121 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6181 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6241 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6301 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6361 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgcca
//
