LOCUS       sequence-205970-        1672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  targeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ 
            mouse strain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Koike K, Yoo SJ, Bleymehl K, Omura M, Zapiec B, Pyrski M, Blum T, 
            Khan M, Bai Z, Leinders-Zufall T, Mombaerts P, Zufall F
  TITLE     Danger perception and stress response through an olfactory sensor 
            for the bacterial metabolite hydrogen sulfide.
  JOURNAL   Neuron. 2021 Aug 4;109(15):2469-2484.e7. doi: 
            10.1016/j.neuron.2021.05.032. Epub 2021 Jun 28.
  PUBMED    34186026
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2021.05.032"; journalName: "Neuron"; date: 
            "2021-08-4- 4"; volume: "109"; issue: "15"; pages: "2469-2484.e7"
COMMENT     Sequence Label: Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV
FEATURES             Location/Qualifiers
     source          1..1672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    6..572
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    23..574
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(167..184)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             592..612
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             613..1641
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
ORIGIN
        1 ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
       61 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
      121 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
      181 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
      241 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
      301 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
      361 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctaagcgta ttcaacaagg
      421 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
      481 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
      541 acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggc acccaagaag
      601 aagaggaagg tgtccaattt actgaccgta caccaaaatt tgcctgcatt accggtcgat
      661 gcaacgagtg atgaggttcg caagaacctg atggacatgt tcagggatcg ccaggcgttt
      721 tctgagcata cctggaaaat gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag
      781 ttgaataacc ggaaatggtt tcccgcagaa cctgaagatg ttcgcgatta tcttctatat
      841 cttcaggcgc gcggtctggc agtaaaaact atccagcaac atttgggcca gctaaacatg
      901 cttcatcgtc ggtccgggct gccacgacca agtgacagca atgctgtttc actggttatg
      961 cggcggatcc gaaaagaaaa cgttgatgcc ggtgaacgtg caaaacaggc tctagcgttc
     1021 gaacgcactg atttcgacca ggttcgttca ctcatggaaa atagcgatcg ctgccaggat
     1081 atacgtaatc tggcatttct ggggattgct tataacaccc tgttacgtat agccgaaatt
     1141 gccaggatca gggttaaaga tatctcacgt actgacggtg ggagaatgtt aatccatatt
     1201 ggcagaacga aaacgctggt tagcaccgca ggtgtagaga aggcacttag cctgggggta
     1261 actaaactgg tcgagcgatg gatttccgtc tctggtgtag ctgatgatcc gaataactac
     1321 ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat ctgccaccag ccagctatca
     1381 actcgcgccc tggaagggat ttttgaagca actcatcgat tgatttacgg cgctaaggat
     1441 gactctggtc agagatacct ggcctggtct ggacacagtg cccgtgtcgg agccgcgcga
     1501 gatatggccc gcgctggagt ttcaataccg gagatcatgc aagctggtgg ctggaccaat
     1561 gtaaatattg tcatgaacta tatccgtaac ctggatagtg aaacaggggc aatggtgcgc
     1621 ctgctggaag atggcgatta gccattaacg cgtggtaccc taagaattcc gc
//
