LOCUS       sequence-205974-        6418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent protein for superresolution imaging and a focal adhesion
            protein for binding substrate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6418)
  AUTHORS   Wang S, Moffitt JR, Dempsey GT, Xie XS, Zhuang X
  TITLE     Characterization and development of photoactivatable fluorescent 
            proteins for single-molecule-based superresolution imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8452-7. doi: 
            10.1073/pnas.1406593111. Epub 2014 May 27.
  PUBMED    24912163
REFERENCE   2  (bases 1 to 6418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1406593111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-10- 10"; volume: "111"; issue: "23"; pages: "8452-7"
COMMENT     Sequence Label: pZyxin-mMaple2
FEATURES             Location/Qualifiers
     source          1..6418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        75..378
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        379..582
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     533..553
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      648..657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2378..2387
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3220..3341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3257..3276)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3311..3330
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3348..3803)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3485..3506)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3697..3716
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3830..3934
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3936..4293
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3936..4127
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3959..3979)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4144..4279
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4328..5122
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4328..5122
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4382..4401)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4992..5011
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5310..5329)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5354..5401
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5730..6318
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6219..6238
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
       61 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      121 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      181 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      241 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      301 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      361 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      421 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      481 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      541 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
      601 atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattcca tggcggcccc
      661 ccgcccgtct cccgcgatcc ccgtttcggt ctcggctccg gctttttacg ccccgcagaa
      721 gaagttcggc cctgtggtgg ccccaaagcc caaagtgaat cccttccggc ccagggacag
      781 cgagcctccc ccggcacccg gggcccagcg cgcacagatg ggccgggtgg gcgatattcc
      841 cccgccgccc ccggaagact ttcccctgcc tccacctccc cttgctgggg atggcgacga
      901 tgcagagggt gctctgggag gtgccttccc gccgccccct cccccgatcg aggaatcatt
      961 tccccctgcg cctctggagg aggagatctt cccttccccg ccgcctcctc cggaggagga
     1021 gggagggcct gaggccccca taccgccccc accacagccc agggagaagg tgagcagtat
     1081 tgatttggag atcgactctc tgtcctcact gctggatgac atgaccaaga atgatccttt
     1141 caaagcccgg gtgtcatctg gatatgtgcc cccaccagtg gccactccat tcagttccaa
     1201 gtccagtacc aagcctgcag ccgggggcac agcacccctg cctccttgga agtccccttc
     1261 cagctcccag cctctgcccc aggttccggc tccggctcag agccagacac agttccatgt
     1321 tcagccccag ccccagccca agcctcaggt ccaactccat gtccagtccc agacccagcc
     1381 tgtgtctttg gctaacaccc agccccgagg gcccccagcc tcatctccgg ctccagcccc
     1441 taagttttct ccagtgactc ctaagtttac tcctgtggct tccaagttca gtcctggagc
     1501 cccaggtgga tctgggtcac aaccaaatca aaaattgggg caccccgaag ctctttctgc
     1561 tggcacaggc tcccctcaac ctcccagctt cacctatgcc cagcagaggg agaagccccg
     1621 agtgcaggag aagcagcacc ccgtgccccc accggctcag aaccaaaacc aggtgcgctc
     1681 ccctggggcc ccagggcccc tgactctgaa ggaggtggag gagctggagc agctgaccca
     1741 gcagctaatg caggacatgg agcatcctca gaggcagaat gtggctgtca acgaactctg
     1801 cggccgatgc catcaacccc tggcccgggc gcagccagcc gtccgcgctc tagggcagct
     1861 gttccacatc gcctgcttca cctgccacca gtgtgcgcag cagctccagg gccagcagtt
     1921 ctacagtctg gagggggcgc cgtactgcga gggctgttac actgacaccc tggagaagtg
     1981 taacacctgc ggggagccca tcactgaccg catgctgagg gccacgggca aggcctatca
     2041 cccgcactgc ttcacctgtg tggtctgcgc ccgccccctg gagggcacct ccttcatcgt
     2101 ggaccaggcc aaccggcccc actgtgtccc cgactaccac aagcagtacg ccccgaggtg
     2161 ctccgtctgc tctgagccca tcatgcctga gcctggccga gatgagactg tgcgagtggt
     2221 cgccctggac aagaacttcc acatgaagtg ttacaagtgt gaggactgcg ggaagcccct
     2281 gtcgattgag gcagatgaca atggctgctt ccccctggac ggtcacgtgc tctgtcggaa
     2341 gtgccacact gctagagccc agacggatcc accggtcgcc accatggtga gcaaaggcga
     2401 ggagacaatc atgtccgtga tcaagcccga catgaagatc aaactgagga tggagggcaa
     2461 cgtgaacggc cacgccttcg tgatcgaggg cgaaggaagc ggcaagccct tcgagggcat
     2521 ccagaccatc gatctggagg tcaaggaggg cgctcccctc cctttcgcct atgacatcct
     2581 gaccaccgcc ttccactacg gcaatagggt gttcaccaag tatcccgagg acatccccga
     2641 ctacttcaag cagagcttcc ctgagggcta cagctgggag aggagcatga catacgagga
     2701 cggcggcatc tgcaacgcca ccaacgacat cacaatggag gaggacagct tcatcaacaa
     2761 gatccacttc aaaggcacaa acttcccccc caatggcccc gtgatgcaga agaggaccgt
     2821 gggctgggag gtgagcaccg agaagatgta cgtgagggac ggcgtcctga agggcgacgt
     2881 gaagatgaag ctcctgctca agggcggcag ccactacagg tgcgacttta ggaccaccta
     2941 taaggtgaag cagaaggctg tgaagctgcc cgacgcccac ttcgtcgacc ataggatcga
     3001 gatcctgtcc cacgacaagg actacaacaa ggtcaagctg tacgagcacg ccgtcgctag
     3061 gaacagcacc gacagcatgg acgaactcta taagtgaagc ggccgcgact ctagatcata
     3121 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3181 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3241 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3301 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt
     3361 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3421 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3481 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3541 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3601 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3661 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3721 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3781 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     3841 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3901 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     3961 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4021 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4081 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4141 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4201 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     4261 ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc
     4321 gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     4381 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg
     4441 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     4501 tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     4561 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     4621 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     4681 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     4741 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     4801 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat
     4861 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt
     4921 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     4981 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga
     5041 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     5101 ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg
     5161 cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     5221 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     5281 ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat accggaagga
     5341 acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt
     5401 cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat
     5461 tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa
     5521 ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca
     5581 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5641 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5701 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     5761 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     5821 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     5881 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     5941 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6001 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6061 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6121 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6181 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6241 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     6301 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     6361 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgta
//
