LOCUS       sequence-205976-        9623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden-Gate compatible fungal transformation vector carrying 
            Hygromycin Resistance w RFP screening cassette (RFP knocked out by 
            GG reaction, allowing for red-white screening).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9623)
  TITLE     pBHt2G-RFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBHt2G-RFP
FEATURES             Location/Qualifiers
     source          1..9623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    99..120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(135..140,141..158,159..165)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    173..189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     197..213
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     197..213
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    344..365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(380..385,386..403,404..410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    418..434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             452..463
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             470..1147
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      1192..1263
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1279..1306
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(1345..1362)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1345..1361)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1354..1376)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    1564..1588
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             2889..3518
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             3947..5020
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      5086..5280
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(5516..5538)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    5624..5764
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     5679..5698
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5779..5796)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5950..6538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6030..6049)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6625..7416,7417..7485))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7248..7267
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    7910..7934
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    8012..8186
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(8217..9242)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANILFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
       61 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
      121 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      181 ggataacaat ttcacacagg aaacagctat gacatgatta cgaattcgga gtgagaccga
      241 tatcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      301 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      361 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      421 tgagcggata acaatttcac acatactaga gaaagaggag aaatactaga tggcttcctc
      481 cgaggacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg
      541 tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc
      601 taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca
      661 gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa
      721 actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgagg acggtggtgt
      781 tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact
      841 gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga
      901 agcttccacc gaacgtatgt acccggagga cggtgctctg aaaggtgaaa tcaaaatgcg
      961 tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa
     1021 aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca
     1081 caacgaggac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg
     1141 tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
     1201 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1261 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta taggtctcac
     1321 gctctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     1381 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     1441 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     1501 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
     1561 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     1621 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     1681 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     1741 cggcttctga cgttcagtgc agccgtctta tgaaaacgac atgtcgcaca agtcctaagt
     1801 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     1861 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     1921 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     1981 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     2041 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     2101 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     2161 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     2221 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     2281 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     2341 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     2401 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     2461 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     2521 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     2581 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     2641 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
     2701 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccagccagc
     2761 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     2821 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     2881 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     2941 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     3001 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     3061 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     3121 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     3181 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     3241 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     3301 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     3361 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     3421 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     3481 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     3541 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     3601 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     3661 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     3721 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     3781 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     3841 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
     3901 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     3961 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     4021 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     4081 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     4141 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     4201 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     4261 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     4321 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     4381 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     4441 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     4501 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     4561 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     4621 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     4681 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     4741 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     4801 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     4861 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     4921 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     4981 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     5041 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggactc tttcctgtgg atagcacgta
     5101 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     5161 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     5221 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     5281 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
     5341 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
     5401 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
     5461 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     5521 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     5581 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     5641 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     5701 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     5761 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5821 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5881 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5941 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     6001 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     6061 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6121 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6181 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6241 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6301 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6361 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     6421 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6481 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     6541 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6601 gattttggtc atgcattcta ggtattacca atgcttaatc agtgaggcac ctatctcagc
     6661 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     6721 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagaac cacgctcacc
     6781 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6841 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6901 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6961 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     7021 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     7081 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     7141 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     7201 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     7261 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     7321 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     7381 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     7441 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatttatt atttccttcc
     7501 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
     7561 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
     7621 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccgcg gtgatcacag
     7681 gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc tccgcgagat catccgtgtt
     7741 tcaaacccgg cagcttagtt gccgttcttc cgaatagcat cggtaacatg agcaaagtct
     7801 gccgccttac aacggctctc ccgctgacgc cgtcccggac tgatgggctg cctgtatcga
     7861 gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg gctggctggt ggcaggatat
     7921 attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg acgtttttaa
     7981 tgtactgaat taacgccgaa ttaattcggg ggatctggat tttagtactg gattttggtt
     8041 ttaggaatta gaaattttat tgatagaagt attttacaaa tacaaataca tactaagggt
     8101 ttcttatatg ctcaacacat gagcgaaacc ctataggaac cctaattccc ttatctggga
     8161 actactcaca cattattatg gagaaactcg aaactggttc ccggtcggca tctactctat
     8221 tcctttgccc tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca
     8281 cagccatcgg tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg
     8341 gctccggatc ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg
     8401 ccgtcaacca agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagg
     8461 cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata
     8521 caagccaacc acggcctcca gaagaggatg ttggcgacct cgtattggga atccccgaac
     8581 atcgcctcgc tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg
     8641 gagccgaaat ccgcatgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc
     8701 agctcatcga gagcctgtgc gacggacgca ctgacggtgt cgtccatcac agtttgccag
     8761 tgatacacat ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg
     8821 attccttgcg gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc
     8881 gcatccatgg cctccgcgac cggctggaga acagcgggca gttcggtttc aggcaggtct
     8941 tgcaacgtga caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaactcc
     9001 ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa
     9061 cgatctttgt agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct
     9121 acatcgaagc tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg
     9181 ctgtcgaact tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc
     9241 atttggatgc ttgggtagaa taggtaagtc agattgaatc tgaaataaag ggaggaaggg
     9301 cgaacttaag aaggtatgac cgggtcgtcc acttaccttg cttgacaaac gcaccaagtt
     9361 atcgtgcacc aagcagcaga tgataataat gtcctcgttc ctgtctgcta ataagagtca
     9421 cacttcgagc gccgccgcta ctgctacaag tggggctgat ctgaccagtt gcctaaatga
     9481 accatcttgt caaacgacac aaattttgtg ctcaccgcct ggacgactaa accaaaatag
     9541 gcattcattg ttgacctcca ctagctccag ccaagcccaa aaaatgctcc ttcaatatca
     9601 gttttggcaa gctgctctag cca
//
