LOCUS       sequence-205982-        7237 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  targeting vector to generate a Trpc2-IRES-taumCherry mouse strain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7237)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the mouse main olfactory 
            epithelium of type B express the soluble guanylate cyclase Gucy1b2.
  JOURNAL   Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19.
  PUBMED    25701815
REFERENCE   2  (bases 1 to 7237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2015-03"; volume: "65:114-24. doi"; pages: " 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19"
COMMENT     Sequence Label: Trpc2-IRES-taumCherry-ACNF TV
FEATURES             Location/Qualifiers
     source          1..7237
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(68..87)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(747..1538,1539..1607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1370..1389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1608..1712)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1738..2193)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1875..1896)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2087..2106
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2320..2342
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2334..2351
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2335..2351
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2358..2377
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2358..2376
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2409..2425
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2409..2425
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
       61 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      121 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      181 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      241 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      301 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      361 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      421 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      481 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      541 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      601 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
      661 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      721 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     1741 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     1801 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     1861 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     1921 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     1981 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     2041 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     2101 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     2161 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     2221 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     2281 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     2341 cgacggccag tgaattgtaa tacgactcac tatagggcga attggagctc caccgcggtg
     2401 gcggccgctc tagaactagt ggatcccccg ggctgcagga attcgatacc atggtatcaa
     2461 gcttaactgc tcactgttgt ttgctgccac ttgctggatc tgtttctttc tgtcccccaa
     2521 cccctgtctt gcaacacttg ttttttttgt ttgtttgttt atttgtttgt tttgagacag
     2581 gatctcacta tgtagctatg gctgtcttgg agcttgcagt atagaccaga gtgggctcag
     2641 attcacagag ctctgcctgc ctctgccttc tcagagctga ttagaggcac gcaccactac
     2701 acgttagagg agggattggc ccctggtaaa ggcactagag aaaccctttg ctaacagaga
     2761 gggattctgg cctagactac ttgccatctg tacccactgg atttaacatc tgaagatgat
     2821 tttgagtgtg agtggggttt gagctaggct ctcaagtaac tcaggatagc cttggatcta
     2881 gaagtgatcc tcctgtctct acccccaagt gctggaatta caggcatgaa ctgtcctacc
     2941 cagccacagg taattttact gaaaaagtct ctactgtggt cttgaggcag tgactttcca
     3001 catctgtcta ttgtttgaca tttattatct tgcacccttc taacagagga cttgctccta
     3061 gctctgcatg cctgtaatct cagtgccgga ggtagaggta ggaggatcag aagttcaagg
     3121 tcattttctg ctgtttaaca agtttgatgc tagctcaggc tccctaagac tttgtgtcaa
     3181 gaagaagaac cgacgaggtg actcaggagg taaaggggct tgccgccaag cctgacgacc
     3241 tcggtgggac tcccaagaag cccatggtga aggcaagagc tgatcctgca agctgtcagc
     3301 ctccacaccc gtgctgtggc atactaagaa cacacgcaca tccatgcaca cagagaaata
     3361 agtaaaaaat aattaataag gagaaaatgt aaacaaacct tcaagcattt tggctgaagg
     3421 tctccggtga gatagatgac gggtttatgg ctcagggtag ccagaaatat tgaaagttag
     3481 taagtagagg taggtctaag agattgggtc aaatttagca agatctccct tgatatacgg
     3541 aggacagagt aacaaaagca gatgatattt acctgtggtg agctagagcc aagatgcagg
     3601 gagtcagatg tcagagggca gagtagtgtg gtctggacaa tgagcacctg atgagtgcat
     3661 tctgctggag gtccttggga aaggcagatc aggaaggact tcctggaaaa ggcagcactg
     3721 gggttattgc ttctgtgctc tacatacagc acacagtcct ctcccaccca gaccaggaaa
     3781 tgttcttaac ttgtctttgt gtcttcctct ttatctccct tgaggtcaca gcaaccaaca
     3841 cgatcttttc acacctaatt cagatgcccc ctccatccag aactcttcct agagttagaa
     3901 ggccatggca gagtctttag ttttattcta tgaggctctt ctctgtgtgg tccgtaacag
     3961 cctctcacaa gccattctct ctccttccct ccccagagcg tgcaggcttc gggacgggac
     4021 cctgcttgtc ttgcatgctt ggccccctgt gttcagtaaa tgttggttga ccaaaagaat
     4081 gaaggtggag gggctggaga gatggtcagc agctaagagc cttactgctc tgcagaggac
     4141 tcgggtgtgg ttcccagcac ccaaactgtg actcagggcc atctgtaact ccaattccag
     4201 gggatccaat gcttcttcct acctctgcag gctcctgcac acgcgtggtg ctcacccaca
     4261 ctcagaacac acacccttat gcaagtaaaa gatgttttta gatcagtact aagaaatgca
     4321 agtgtccata ttcacaaggg tggctcaaaa ggcttcatgg acatagaaca cctttggctg
     4381 catcttcagg ggaaggaaat gtgttctggg tagatcccag gtttctctcc caacctggtc
     4441 cccgttttct tctcctttgt ttgggccacc atgccagttt caacgaaacg ttccagtttc
     4501 tcttttggac catgttcggc atggaagagc acacagtggt ggacatgcct cagttcctgg
     4561 tgcctgagtt cgtgggcagg gccatgtacg gcatctttac catcgtcatg gtcattgtgc
     4621 tacttaacat gcttattgcc atgatcacca actccttcca gaagatcgag gtgcgtgacc
     4681 tgagccaggc tccgtgggga gggacagggc gcgaagaccc tcgcggtcct gctctgctct
     4741 cacttttatt ttatggcttt gtacaaattc tcctctgtac aacaagggtt tcagcactgc
     4801 tgttgctatg ggcagactcc aagtctaatg ctctgtgcgc ttttccttgg tggtagtggt
     4861 ggtggtggtt gtggcggtgg tggcggcagt ttagagcatt gtataaaagt taagtgggaa
     4921 cacaagaagg aggcagatgg gttcacattg cggcctggag aataggttgc acactgagta
     4981 gcaactggaa attagagggg aaaggaaaat gggagaggag agtgtgggtg gttggtgggg
     5041 aggaaacaag aacaagaggt tggcaccctg gagggtagga gaggggaaga tggcagagca
     5101 gccacctgag tgagcagagc ggccatgcca gcgccagcgt ggacctagcc ttgcctcctg
     5161 cctcttggtc cccaggatga tgctgatgtg gagtggaagt ttgctcgctc caagctctac
     5221 ctgtcctact tccgagaggg tctgacgctg cctgtgccct ttaacatcct gccatcccca
     5281 aaggccgcct tctacctcgt caggtgatgg cccctgtaca ccacccagcc tgccccattt
     5341 tcccaggccc tatccccccg gccccacccc cggccccgct acacacacac acatacatgc
     5401 acacacacac actaacgtct tttcaccatg gcaatctctc aggagaattt tccggttcct
     5461 ttgctgtggc tcctcctgct gcaaagccaa gaagtcggac tacccgccca tcgggacctt
     5521 tgtgggtacc ttttctgtct gtcatttccc cacctgtgtt acctaagcca tcagtatgtc
     5581 cccccagagc ctgcttccaa ttcccgctca cacggcctcc gttttcatcc gcctgcttct
     5641 ggctgaatcc tacatctcta gtccacatcc ggcagcctaa gcataaccca tggcacctcc
     5701 atcccacgcc cgaggtccag actgtaagac atgctcgggt gatttaacag cacttgggca
     5761 aagccccctg aggaatgaga ggccgctggg catccaaggg gctcacctac ctcctatcat
     5821 ctgcaccata actcagactg tctgagcatg agcgctttcc cagtttggac cagtgctgca
     5881 gttcttcagg ggtctttcct tcccacacca cccccacctc tcagtcttca ccacatactc
     5941 tagacacact gtcctctcac agtctgctgc ccccagtcgg cccccctcag tcctgcagga
     6001 ctgcactgca ccagacaggc cagggacaga aagcctgcct agattttgcc tttctccttt
     6061 acttggtcag ggcagagatg gaaaggaagg tagagagttc actctgcctt ccattgcaga
     6121 ccaaccccgg ggcaagggcg ggctccgccg gggaaggaga acgcgtgtcc taccgccttc
     6181 gagtcatcaa ggctctggtg cagcgctaca tagagactgc ccggcgcgag ttcgaggaga
     6241 cccgtcggaa aggtcagtgg ctcacttgtg atgcaacccc tttgctgtcc ttggctcgcc
     6301 ttggaagagt gtccacaccc agactcaccc tccacacttc tgcattaggc tctagtcccg
     6361 tccttcctac cctagaaagg cacagcttat ctgttactct gcctttcata ttgctttgtt
     6421 ctcattcctt catttatgtg tgctcagata ttgatctcga gtttcccccc tccccctccc
     6481 atttccttct tcctgtctta ccaagttgcc caggctcatc ttgaactcac tttgaagcct
     6541 aggctggctt tgaactctcc atcatcttcc ccagtctccc aactagatgg gactgcaggc
     6601 ctgcactacc aaccccagct aaggctttcc ttctttgtca caggctgtcc ttaaattaat
     6661 actcacattt acctgacccc tcagttcttg cagtccccct ttagcatcca gacagggtgt
     6721 ccctgggttc agtgggggtg gggtgcagat agacagaaga ggtactgagc aggctccctt
     6781 ctgaaagacc tgccctgatt cctctcagac ctgggcaaca gactgacaga gctgaccaag
     6841 actgtgtctc gactgcaaag cgaggtggcc agtgtgcaga agaacctggc ggcgggaggg
     6901 gcaccacggc ctccggatgg tgccagcatc ctcagtagat acatcacccg agtgcgcaac
     6961 agcttccaga acctgggccc ccctacctct gacaccccag cagagctgac tatgcctggg
     7021 attgtggaga ccgaagtctc tttaggagat ggccttgatg gcacaggtga agctggagct
     7081 cccgctcctg gagagcccgg ctcttcctcc tctgcccatg tgctggttca cagggagcaa
     7141 gaagcagagg ggtcagggga cttgcccctg gaaggagatc tggagaccaa gggcgagtcc
     7201 taaggcaaca gaagggtctc ttctgtcact aagtgca
//
