LOCUS       sequence-205983-       11580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  targeting vector to generate a Trpc2-IRES-taumCherry mouse strain..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11580)
  AUTHORS   Omura M, Mombaerts P
  TITLE     Trpc2-expressing sensory neurons in the mouse main olfactory 
            epithelium of type B express the soluble guanylate cyclase Gucy1b2.
  JOURNAL   Mol Cell Neurosci. 2015 Mar;65:114-24. doi: 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19.
  PUBMED    25701815
REFERENCE   2  (bases 1 to 11580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Neurosci."; date: "2015-03"; volume: "65:114-24. doi"; pages: " 
            10.1016/j.mcn.2015.02.012. Epub 2015 Feb 19"
COMMENT     Sequence Label: Trpc2-IRES-taumCherry-ACNF TV
FEATURES             Location/Qualifiers
     source          1..11580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    12..563
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(156..173)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     regulatory      1739..1748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1745..2455
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1890..1908)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2158..2177
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    2490..2523
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2550..2598
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     CDS             complement(2605..3408)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2716..2735)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3326..3345
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     polyA_signal    4146..4194
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     intron          4544..4609
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(5435..5455)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(5479..5490)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    6165..6198
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(11069..11085)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11070..11086)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(11114..11132)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11153..11169)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11153..11169)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11166..11188)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11177..11193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11201..11207,11208..11225,11226..11231))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11246..11267
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11384..11401)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
       61 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
      121 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
      181 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
      241 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
      301 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
      361 gatagttgtg gaaagagtca aatggctctc ctaagcgtat tcaacaaggg gctgaaggat
      421 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
      481 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
      541 ctttgaaaaa cacgatgata atatggccac aaccatggct gagccccgcc aggagttcga
      601 cgtgatggaa gatcatgctc agggggacta caccctgcaa gaccaggagg gtgacatgga
      661 ccccggcctg aaagagtctc ccctgcagac cccggccgat gatggatctg aggaaccagg
      721 ctctgaaacc tctgatgcta agagcactcc gacggcggaa gatgcgacag cacccttagt
      781 ggatgaggga gcccccggtg agcaggcggc cgctcaggcc cccgcggaga tcccagaagg
      841 aaccgcagct gaagaagcag gcattggcga cacgtccaac ctggaagacc aagctgccgg
      901 acacgtgacc caagctcgca tggtcagtaa aggcaaagat gggactggac ccgatgacaa
      961 aaaaaccaag ggggcggatg gtaagcctgg aacgaagatt gccacacccc ggggagcagc
     1021 ccctccaggc cagaaaggcc aggccaacgc cacccggatt ccagcaaaaa ccactcccac
     1081 cccgaagacc tcgccaggtg aatctgggaa atccggggac cgcagcggct acagcagccc
     1141 cggctcccca ggcactccgg gcagccgctc ccgcacaccc tccctgccga ccccgcccac
     1201 ccgggagccc aagaaggtgg cggtggtccg cactcccccc aagtcgccgt ctgcagccaa
     1261 gagccgcctg caggccgctc ccgggcccat gccagacctg aagaacgtca agtccaaaat
     1321 cggctccacg gaaaacctga agcaccagcc aggaggtggc aaggtgcaga taattaataa
     1381 gaagctggat cttagcaacg tccagtccaa gtgtggctca aaggataata tcaaacacgt
     1441 gccaggaggc ggcagtgtgc aaatagtcta caaaccagtg gatctgagca aggtgacctc
     1501 caagtgtggc tcattaggca acatccatca taagccagga ggtggccaag tggaagtgaa
     1561 atctgagaag ctggacttca aggatagagt ccagtcgaag attgggtccc tggacaacat
     1621 cacacacgtc cctggcggag ggaataaaaa gatcgaaacc cacaagctga ccttccgcga
     1681 gaacgccaaa gccaagaccg accacggggc ggagatcgtg tccggggatc caccggtcgc
     1741 caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     1801 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     1861 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     1921 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     1981 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     2041 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     2101 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     2161 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     2221 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     2281 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     2341 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     2401 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtaatctag
     2461 agattcgaga tctagatatc gatgaattca taacttcgta taatgtatgc tatacgaagt
     2521 tatggatctg tcgatcgacg gatcgatccg aacaaacgac ccaacacccg tgcgttttat
     2581 tctgtctttt tattgccgat cccctcagaa gaactcgtca agaaggcgat agaaggcgat
     2641 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     2701 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     2761 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     2821 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag
     2881 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     2941 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     3001 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     3061 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     3121 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     3181 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
     3241 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     3301 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     3361 ggccggagaa cctgcgtgca atccatcttg ttcaatggcc gatcccatat tggctgcacg
     3421 gatcctgaac ggcagaggtt acggcagttt gtctctcccc cttccgggag ccaccttctt
     3481 ctccaaccgt cccggtcgcg ctctcggcgc ttctgaggag agaactggct gagtgacgcc
     3541 ctttatagat tcgcccttgt gtcccgcccc ttcctttccc gccctccctt gcgctacggg
     3601 gccgcccgca ccggcctaca cggagcgcgc gcggcggagt tgttgacgct agggctccgg
     3661 ctccctggtt gggtgttctt tctgacgcga caggaggagg agaatgttcc tggtcctgtc
     3721 gtcctccttt cgggtttccc gtgcactcaa accgaggact tacagaacgg aggataaagt
     3781 taggccattt ttactcagct tcggagttca ggctcatttt cagctaaagt ctctcattag
     3841 tatcccccca cacacatcgg gaaaatggtt tgtcctacgc atcggtaatg aaggcggggc
     3901 ccttcgggtc ctccggagcg ggttccgggg gtggggggaa ggagggaggg acgggacggg
     3961 cctcgttcat gaatattcag ttcaccgctg aatatgcata aggcaggcaa gatggcgcgt
     4021 ccaatcaatt ggaagtagcc gttattagtg gagaggcccc aggacgttgg ggcaccgcct
     4081 gtgctctagt agctttacgg agccctggcg ctcgatgttc agcccaagct ttcgcgagct
     4141 cgaccgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgctcagc
     4201 tttacagtga caatgacggc tggcgactga atattagtgc ttacagacag cactacatat
     4261 tttccgtcga tgttgaaatc ctttctcata tgtcaccata aatatcaaat aattatagca
     4321 atcatttacg cgttaatggc taatcgccat cttccagcag gcgcaccatt gcccctgttt
     4381 cactatccag gttacggata tagttcatga caatatttac attggtccag ccaccagctt
     4441 gcatgatctc cggtattgaa actccagcgc gggccatatc tcgcgcggct ccgacacggg
     4501 cactgtgtcc agaccaggcc aggtatctct gaccagagtc atcctaaaat acacaaacaa
     4561 ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc ttagcgccgt
     4621 aaatcaatcg atgagttgct tcaaaaatcc cttccagggc gcgagttgat agctggctgg
     4681 tggcagatgg cgcggcaaca ccattttttc tgacccggca aaacaggtag ttattcggat
     4741 catcagctac accagagacg gaaatccatc gctcgaccag tttagttacc cccaggctaa
     4801 gtgccttctc tacacctgcg gtgctaacca gcgttttcgt tctgccaata tggattaaca
     4861 ttctcccacc gtcagtacgt gagatatctt taaccctgat cctggcaatt tcggctatac
     4921 gtaacagggt gttataagca atccccagaa atgccagatt acgtatatcc tggcagcgat
     4981 cgctattttc catgagtgaa cgaacctggt cgaaatcagt gcgttcgaac gctagagcct
     5041 gttttgcacg ttcaccggca tcaacgtttt cttttcggat ccgccgcata accagtgaaa
     5101 cagcattgct gtcacttggt cgtggcagcc cggaccgacg atgaagcatg tttagctggc
     5161 ccaaatgttg ctggatagtt tttactgcca gaccgcgcgc ctgaagatat agaagataat
     5221 cgcgaacatc ttcaggttct gcgggaaacc atttccggtt attcaacttg caccatgccg
     5281 cccacgaccg gcaaacggac agaagcattt tccaggtatg ctcagaaaac gcctggcgat
     5341 ccctgaacat gtccatcagg ttcttgcgaa cctcatcact cgttgcatcg accggtaatg
     5401 caggcaaatt ttggtgtacg gtcagtaaat tggacacctt cctcttcttc ttgggcatgg
     5461 ccgcaggaaa gcagagccct gaagctccca tcaccggcca ataagagcca agcctgcagt
     5521 gtgacctcat agagcaatgt gccagccagc ctgaccccaa gggccctcag gcttgggcac
     5581 actgtctcta ggaccctgag agaaagacat acccatttct gcttagggcc ctgaggatga
     5641 gcccaggggt ggcttggcac tgaagcaaag gacactgggg ctcagctggc agcaaagtga
     5701 ccaggatgct gaggctttga cccagaagcc agaggccaga ggccaggact tctcttggtc
     5761 ccagtccacc ctcactcaga gctttaccaa tgccctctgg atagttgtcg ggtaacggtg
     5821 gacgccactg attctctggc cagcctagga cttcgccatt ccgctgattc tgctcttcca
     5881 gccactggct gaccggttgg aagtactcca gcagtgcctt ggcatccagg gcatctgagc
     5941 ctaccaggtc cttcagtacc tcctgccagg gcctggagca gccagcctgc aacacctgcc
     6001 tgccaagcag agtgaccact gtgggcacag gggacacagg gtggggccca caacagcacc
     6061 attgtccact tgtccctcac tagtaaaaga actctagggt tgcggggggt gggggaggtc
     6121 tctgtgaggc tggtaaggga tatttgcctg gcccatggag atccataact tcgtataatg
     6181 tatgctatac gaagttataa gctttcgcga gctcgaatcg gatccgaatt cttaattaag
     6241 gtaccggcgc gccttaatta actgctgtgt tgggtagagc cagggtctca cagtagaaga
     6301 ccatggtgct ttttaatatt attattattt attttatttt attttatttt atttgttttt
     6361 ttgcctcagt aaaatttctg ctgtggaagt cagatggtag tctccatctc ttggcatcct
     6421 aagattcctt cctttacttc ccctaaaatg gacgctgtgg aaggagggaa gagagaaaca
     6481 gcttgctttg agttctgggg tttagaatcc tacttttggg attccttagg tctttagtga
     6541 aaaacgttct ggcctaggag actggaagac tgggagctag gaggccagag aagcaggaga
     6601 cctgagtggg taggtctgag agatgggagg cctggaggtg gtgccctttc ttggcctggc
     6661 attctcttca gggatgggta tcaatctatc ccctccctga cttcatgagg cccctgtggc
     6721 tgttaagaag catcaggcac agaggctaca atgtgtgacc tggatgactg ggaaattagt
     6781 ttccatccct cgactttcaa gcttgtcaag gacaaatgac gtcatgcaat aaaccagtca
     6841 taaaagccat gtctccacac cacaactctg tttaattgag tagccatcca gtctttacct
     6901 tgcatgtcct aggaagttcc caccacactg tggggttccc tcaaccctgc catggcctca
     6961 gatttccaac tctggttgga caggtggctc ctaggactgg aaccagaacc acactcctgc
     7021 ttcctgcagc cgttcaaagc cccttcttca tagtaaggtc attagttccc aagagtccta
     7081 agaggctttc agggtccagc tctggagagc tgcagcttcc ttgggtccaa gagcagtgtc
     7141 tttcctgatt gcagagccag agcctctgtg gagggtgcct ctggctgccc gactgctgga
     7201 gaaaggagaa aagatggtga ggcggggcag cagggccagt gaagctggaa caagtctcag
     7261 agcaggctgg agggtgacct acctcttgag gacacacagc catgtctctt ctcccctgca
     7321 acagatagca tagtaagacc ccggggtctt ctcctcaaag caggccctcg ctccccttca
     7381 gcttctcccc gggcaacaat tccaggttgc ctgtccatcc ttgctcctac ccacacatct
     7441 gtcactcttc cactgtcccc gcctccacaa ctgaacctgg cacttcctac cttgttagcc
     7501 ccatcaagtc agagcctact gagaccggaa gcaatattgc ctccttctcg ctgttccttg
     7561 gtctacgagg tactacagag gaccttttga gaagacagtc atctgaggat gtccacagat
     7621 cttgaactct cccaggacct ggtggagccc caatctcact aaccaagtgg ttcctgaagg
     7681 ctctggggac tgggtcagaa tacactggct tctttcaccc accctgccat tctctgtctc
     7741 ccattcatcg ctcaccaccc aaataggcgc agttaaagtg gctgcaggtc tgatcataac
     7801 tcgagagagt cactatgctc tgggctccat cctgggagaa cccagtgacc aagaagactt
     7861 cgtgtggggg tatcagcacc tcacgctcct cagggaagac agacaacgcc tggataggag
     7921 ccccaaaaca agtccttaga ttgaagaagg tggcattccc aaacctgcga gccactctct
     7981 catccacaga gctggaggta aactgtccta atcggacaga gtcccccagc ctcttgggtt
     8041 caaagtgaag actgcccaca cctcggaaca ccacctcccc aggtccccta ctgcaccctc
     8101 cagagccccg cagcagctgc agagcccggg tcaagtagaa atgcagggcc ttgaagggga
     8161 agtgcctcat gtagagctcc cgggagccac ctcctgtccg tacggcctgg ttcagctccc
     8221 agtacaaggt gttggatgag ttggtgtaca ccataatcgc tactccgtgc tgggctttga
     8281 agccaggagg tagagtgagc ttgtgacgcc ggtgtgccca ggcctcttgt gctgcttccc
     8341 aggattcccg cagcagggca tggcgtgcca tctcttcctt tagcagcagg cctgctttct
     8401 cctccatctc ctcagagcag cccacatagg catcatcaaa ggtatctgga accaggctca
     8461 ggggcaggat aggaacagct cgaacctatg gacacaaggg aggtgccaca gaggccaaag
     8521 tgtaagttta aaacactggc cagggactgc tggggacagg ttttcatttc tgtgcgacat
     8581 agctgatgca tgcaactggc aggatccgaa acagacacaa ataacacagg caatggagtc
     8641 ggacagccat gggtttgtgt aagcaccacc tgctggctct gggatctgga cttcatgttc
     8701 ttcacctatg gattaaggat gcttccagcc tcacagggtt gtgaggagag catgaggata
     8761 attgcggcac aacctagagg ggcggcatct gaccaagaat cgagtctgcc tggttcctgt
     8821 gctttcctgg cactgctatt ccgtatgcag gcaatgtgca gccagtgtct gatccttgcc
     8881 ctggtcccca accctcttag ccatttctca tcctcgggga agccctgcag ttcagtatac
     8941 tttactgtca gctccggggt tcctgcatcc tctctacttt agaaggcttc atcctaaact
     9001 caaggaaggg aggagtgttt tgtcctcagc acaaccatac tcccagagtg tgatgtggga
     9061 ggggagaggg cctgccaggg tctacagcag catacccgtg tggacagggg tttgcctgca
     9121 ttgagcctgt ggtatgcagt gggaaggaga aaggtggagg caatctctat ggaggggtag
     9181 catggaagga gacttcacac ggggatcagg gagcacaggg aggtggtatt ctgggagaat
     9241 gacctggcac ttggattccc taatggaata gaagttcctg agaagaggac tgggaggagg
     9301 gaagagcctc ttgggaaggg tttccgttat cattcctcct ccaccaccac cttctcagcc
     9361 tctgacccac aactgacttt gttgacaaag gatagtcgct agagttacct tccagagggc
     9421 gtgcaagctg aggcagctga ggaccatcag cagatcctcc agaatcatcc taggacgagt
     9481 cccgagacag caggaccctg atgatgatga agccagtgga ggggagtgct ggcttggatg
     9541 ggctccagga ccttccttcc ttcagcctag gagggtcctt agtcaaggac tggaactcag
     9601 gagggatgag gggtgaagct gaggatgagt ttaaggatgg atgtggcttc agactttatt
     9661 gtccccatgt cagccctagt ctatgactac aagatggccc cacacccagg gtctccagga
     9721 cttctttgcg atggtatagg tgggccactt atatgactct gccgcaagag gggcggggct
     9781 gggcggggca gggatgggaa cccacctgga agaagttgag acagagagcg gcccggccca
     9841 gggctatggt tctttgcggc tgactggagc tgaagtgccg gcaggcgctc gcagatcgca
     9901 gatcctgaac aaacagctgg acaaagtccc cggggctaac tatagcccct ggactggtaa
     9961 cccgacacct atcttcccca acaacagaca ggggcgtggg agctgaactg gcagagtcct
    10021 tgggtgattg aggggcaaaa ctctccttcg tttggatgat aggttctttc tgtaagatct
    10081 gaactaagtc aaagttgggg gagggacctg tgagacttgg tctcttctcc ggtggccctg
    10141 ggccgggcca tggccctcag acccccaggc cacgaagccc cacgaggatc tctccttgtt
    10201 cattacgcta gttaagtcgc caaatctgtc ttatttgcat acaatttgca tacgacacac
    10261 gttgggtcac gtgcaccagc atcaaccctc caccccaccc ccacttcctt ccctactctt
    10321 aaccttcttt gtctcctacc ctggtccgaa gggccagggg tagaagggaa tttatgacat
    10381 tcagtgctta gccgtccagg aacagccaca ccccttccac cccctcagac attccctttc
    10441 cagacttctc cggctcagct gaacccatgt ttgtaaacac agctttctct cgcacctccc
    10501 ttgtgcttga ctataaataa cctagttctg agttcagatg cccagttggg tgtaatggga
    10561 gaaggaccca gcttgcggga aggtgaagga gggacgttct taacaccaag ggttagagat
    10621 gcctcctgtt ggggccacca ttagcatagg ataaggcctc tccagtccag ctcatgaatt
    10681 tacccatcac caccctttac cacctcatat tgtcagccaa ataaagtttc cttcacccaa
    10741 aagcctcttt tctcctccaa tccttcctga aggctaaaga gaaaaacaaa ctatgtccag
    10801 cagtgatccc ttccttccct cctgacccca tcctcccttt ctgggaggaa ttcaggtact
    10861 tgcagtttct gggacagcag atgctgtgtg ggggcagcct atggcttggg gattttctta
    10921 tatcctagca cccggaactc caagaagtgg agtcaaatct ggtcacgtgt gggtcaggga
    10981 tcctgagagg ggcagcatcc tcccaggtca cacggtgagt cagagatcca gcaggaagct
    11041 aggttcttta gggcagaaca aagcttatcg ataccgtcga cctcgagggg gggcccggta
    11101 ccagcttttg ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc
    11161 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    11221 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    11281 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    11341 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    11401 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    11461 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    11521 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
//
