LOCUS       sequence-20599-e         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG306GAL-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(42..166)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(174..190)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(174..190)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(205..646)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(211..234)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    529..646
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 aagcataaag tgtaaagcct gggggtgcct aatgcggccg ccatagtgac tggatatgtt
       61 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
      121 ttatatcatt ttacgtttct cgttcagctt ttttgtacaa acttgtgatg ggggatccac
      181 tagttctaga atccggggtt ttttctcctt gacgttaaag tatagaggta tattaacaat
      241 tttttgttga tacttttatt acatttgaat aagaagtaat acaaaccgaa aatgttgaaa
      301 gtattagtta aagtggttat gcagtttttg catttatata tctgttaata gatcaaaaat
      361 catcgcttcg ctgattaatt accccagaaa taaggctaaa aaactaatcg cattatcatc
      421 ctatggttgt taatttgatt cgttcatttg aaggtttgtg gggccaggtt actgccaatt
      481 tttcctcttc ataaccataa aagctagtat tgtagaatct ttattgttcg gagcagtgcg
      541 gcgcgaggca catctgcgtt tcaggaacgc gaccggtgaa gacgaggacg cacggaggag
      601 agtcttcctt ccgagggctg tcacccgctc ggcggcttct aatccgtact
//
