LOCUS       sequence-206-exp        5034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3790
FEATURES             Location/Qualifiers
     source          1..5034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1405..1433)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2181..2201
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        2779..2883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2884..2952,2953..3744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3102..3121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3915..4503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4404..4423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4657..4674
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4791..4812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4827..4832,4833..4850,4851..4857)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4865..4881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4870..4892
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4889..4905
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4889..4905
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgaggcattt gaattggggg tggtggacag taactgtctg
       61 taataataat tactcctgac caggttgcaa ttcgagtttt gataagcata attatacctt
      121 gtacattgtg ggttttgtgc tgtggacgtt ttattgtgac atccccataa gctacaagaa
      181 caaaaatgaa atgtaaaagt attgaaaacg tcgtacattt tatatctgag tagtatcctt
      241 tgctttaaat gtccataaaa ctaattttat aatcaataaa acaacgtttg taaatcaact
      301 gagtttacaa gtagagacat tgagggatac tttcactatg ctaaagtgat aatcgaccaa
      361 ataataactt cactttggta tttattcctg tcttataaat gttatgtatg aattaaattc
      421 atatgcatat ggctcactct gacaattttt aataatcttc cagatcaata ttgactaccg
      481 atgcgggtgg tcttttgctt tgaattctgc tgaactttac accccgaaca gcaatgtgtg
      541 cttcagccta aaaaaaagta agtgtgttaa tcagtgcccc cgattcttca ttttttgccc
      601 ctctctcccg tttcgtcggc aaaagaagag aaaataaaga taagtctcaa gataggttgg
      661 taatcgctaa agtggttgtg tggataagag tagcaaaatg gcaggaagag cactttgcgc
      721 gcacacactg tactcattgt tctggataaa attctctcgt tgtttgccgt cggatgtctg
      781 cctctctgca ttgagccggc ttcttcacta tctttagtta acctaaaatg ccgtttcttt
      841 tctcgtatcc ccactatccc gttgaggttc tctgctctct tcgctcccta ccgccagcga
      901 gcaactatcc gtgggggcgc cttgctcgga agatgggggg gaagaaagaa gatttttgct
      961 atttgcactt gagaaagaga cttttcctgc gtcgatggtt agagaacagt gtgcagacac
     1021 ttttcagcta cctagaatta caattggata tccccgcctc ccaatccacc cacccaggga
     1081 aaaagaaggg ctcgccgaaa atcaaagtta tctccaggct cgcgcatccc accgagcggt
     1141 tgacttctct ccaccacttt tcattttaac cctcggggta cgggattggc caaaggaccc
     1201 aaaggtatgt ttcgaatgat actaacataa catagaacat tttcaggagg acccttggct
     1261 agcgtcgacg gtaccggtca gatatcactc gaggagctcc cgagatccta tcgattcgcg
     1321 gccgctgtac acccgggtgc cctatagtga gtcgtattgg taccggtaga aaaaatgagt
     1381 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     1441 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     1501 acccttaaat ttatttgcac tactggaaaa ctacctgttc catgggtaag tttaaacata
     1561 tatatactaa ctaaccctga ttatttaaat tttcagccaa cacttgtcac tactttctgt
     1621 tatggtgttc aatgcttctc gagataccca gatcatatga aacagcatga ctttttcaag
     1681 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
     1741 tacaagacac gtaagtttaa acagttcggt actaactaac catacatatt taaattttca
     1801 ggtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     1861 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     1921 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttgtaag tttaaacatg
     1981 attttactaa ctaactaatc tgatttaaat tttcagaact tcaaaattag acacaacatt
     2041 gaagatggaa gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
     2101 cctgtccttt taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc
     2161 aacgaaaaga gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat
     2221 ggcatggacg aactatacaa aagcccacaa gctttggaat tcgctagccg gccatacaag
     2281 taatccggat gatcgacgcc aacgtcgttg aattttcaaa ttttaaatac tgaatatttg
     2341 ttttttttcc tattatttat ttattctctt tgtgtttttt ttcttgcttt ctaaaaaatt
     2401 aattcaatcc aaatctaaac attttttttt ctctttccgt ctcccaattc gtattccgct
     2461 cctctcatct gaacacaatg tgcaagttta tttatcttct cgctttcatt tcattaggac
     2521 gtggggggaa ttggtggaag ggggaaacac acaaaaggat gatggaaatg aaataaggac
     2581 acacaatatg caacaacatt caattcagaa atatggagga aggtttaaaa gaaaacataa
     2641 aaatatatag aggaggaagg aaaactagta aaaaataagc aaagaaatta ggcgaacgat
     2701 gagaattgtc ctcgcttggg cccaaaaggc cttttttttt tggcgccgac gtcaggtggc
     2761 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     2821 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2881 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     2941 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     3001 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     3061 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     3121 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     3181 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     3241 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     3301 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     3361 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     3421 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     3481 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     3541 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     3601 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     3661 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     3721 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     3781 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     3841 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     3901 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     3961 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4021 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     4081 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4141 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4201 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4261 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc
     4321 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4381 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4441 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4501 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4561 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4621 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     4681 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     4741 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     4801 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     4861 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     4921 ctgtaagttt aaacatgatc ttactaacta actattctca tttaaatttt cagagcttaa
     4981 aaatggctga aatcactcac aacgatggat acgctaacaa cttggaaatg aaat
//
