LOCUS       sequence-206017-        9558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NRPS module containing CATR domains with C-terminal 6His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Schneider BA, EP Balskus
  TITLE     Discovery of small molecule protease inhibitors by investigating a 
            widespread human gut bacterial biosynthetic pathway,
  JOURNAL   Tetrahedron
REFERENCE   2  (bases 1 to 9558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Tetrahedron"
COMMENT     Sequence Label: pET29b-RupA_C2-A2-T2-R-CHis
FEATURES             Location/Qualifiers
     source          1..9558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(560..1375)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(560..1375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1283..1302
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1367..1386
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1497..2085
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1986..2005
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2239..2256
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2271..2413
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2339..2358)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2499..2521
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2515..2706)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3515..4597)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4559..4578
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4598..4675)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4881..4900
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     4988..5007
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4988..5006
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5007..5031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5046..5068
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5079..5123
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             9402..9419
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9472..9490)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9486..9533
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatgtcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatcga tctcgatccc
     4981 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
     5041 ataattttgt ttaactttaa gaaggagata tacatatgaa agaaaccgct gctgctaaat
     5101 tcgaacgcca gcacatggac agcccagatc taatgttcag cggtacggca aatcagaaaa
     5161 ctgtcaaaaa agaagaaaag aaagaacata aggctctcta tcctctttca aagactcagc
     5221 tcggcgtgta tattgactgt atgaaaaatc cgtacagcac actttacaat attccgtcaa
     5281 ttctcaaatt ttcaaaatca gtcgatgcac aaaagcttgc cgactgcgtt gtaaaggtaa
     5341 tcaaggctca cccatatatt atgactcacc tttcacttga aaacgatgat attgagcagg
     5401 catatgttga ctctgcaaaa ccgaatgtcc ccgttgaaaa gcttacggaa gaacagcttg
     5461 aatcctacaa aaaggatttt gtaaaaccgc acaatcttat gaaatcaccg ctgttcagaa
     5521 tctcggttgc agaaaccgaa aaggcggtat atctgctttc agatttccac caccttatct
     5581 ttgacggtgc gtccgttgca cttttttttg aacagcttaa aacgctgtac gaagggggta
     5641 atattgagcc tgaaagctac acctactttg attatgcaga gaatgaaatt aaggctgaaa
     5701 gcagtgatga atttagaaat gctgaaaagt tcttcgacaa tatgatgaaa agttttgaat
     5761 ctgcaagcga aatcacggct gacctcagag gtcatgcaga ggacggtgct cttgcatctc
     5821 aggcagtccc cgttgatatg gcaagggttg aaaacttctg ttcacagcac ggaatcactc
     5881 ccgcccacct tttccttgcc ggcacattct acgctgtttc aagatttgtg aacagcagaa
     5941 atgtttacat cagcacaatc agcaacggca gaagcgatat gcggcttaca aactgctttg
     6001 gtatgtttgt aaaaactctt gcgctcggca ttgaaattga ggacatcacc tcacttgaat
     6061 ttgttgaaaa aagcaaagct gttttcacgg attcaattga aaacgaaatt tatccctatg
     6121 cacagctttg tgcaaaatac ggctatgcgc cgaacattat gtacgaatat cagcttggtg
     6181 ttgttgataa ccttgaaatt gacggcaaag ccgttgtccg tgattacctt gaaatgaata
     6241 ctgcaaaatt taaaacagcc gttcacattg aggattacaa gggcaagccg agcgttgtgg
     6301 ttcagtacaa tgacgcactt tacagcggtg agcttatgag aacgcttgca aaatccgttc
     6361 tctgcgctgt tgaacatatt atcgaaaatc caaacggcaa aatcagaaag gtttcactcc
     6421 ttgacaacgc cgcaattgct cagcttgaaa gctttaagtc aacagaaatt gctcccgtta
     6481 agacaaaact tcttcacaaa atgtttgaag aacaggttgc aaaaactccc gacagaattg
     6541 ctctttcggc ttgtgacggt aaactaacct ataaagagct tgaccgtctt gcaaatatca
     6601 cggcaaactc actcattgaa aaaggtcttg aaaaaggcgg aaaggttctt attctgcttg
     6661 aaagaacctc aaaattcttc atatcacttt tcggtattct caaggccggc ggtgcgttta
     6721 ttccgtcatg tcccgactat ccgaaggagc gaatcgacag tattattgag gacagtgacg
     6781 cagactttgt aattacgaac ggcgaactgc tcaataaata tgaccgcacg gttgatgttt
     6841 caaaacttct ttcaggcaat aatgaagaaa atccgaatgt tgatgtacag cctgacgacc
     6901 ttgcgtacct tatctacacc tcaggttcaa caggtaagcc aaagggcgtt atgctacgcc
     6961 acatcggcat tgcaaactac cttgcataca gcgattcaaa cattcaggtt aaagaggttg
     7021 ttgacaactg tcacgcatac ggctctgtaa ctacaatcag ctttgatatg tcgctaaagg
     7081 aaaccatgct gtcgctctgc aacggtctta cactcgtttt tgcaagtgaa gaacagacgg
     7141 taaacccgat gtcacttgca gaattcttta aagaaaacaa tgttgatgcg ttcaactcta
     7201 caccgtcaag acttttgcaa tatatggagc ttgacgaatt tgcagaagca atggcaaact
     7261 gcaaggttat tctctcgggc ggtgaaaaat atcccgacaa gctattaagg atactgcgtg
     7321 aaaagacgaa cgcaacaata atcaacacct acggtccgac tgaaatcacg gtttcttcaa
     7381 acgcaaaaaa tcttacgaat gcagacgaaa tttcaatcgg caggccgtta cgcaactaca
     7441 cggaatatat cgttgactca gacaacaatc tccttccaat cggagttgtg ggcgagcttc
     7501 tcattcaggg atgcggtgtg gcactcggct acaacaaact tcccgaacag actgcaaagg
     7561 cattcatcga atttaacggt gaacgcacct accgctcggg cgactacgca aaatggacaa
     7621 cagacggcga tgttattatt ctcggcagaa ccgacaatca ggttaagctg agaggattaa
     7681 gaattgaact cggtgaaatt gaaaagtgtc tgaccgcagt tgacggaatc aagagcggaa
     7741 ttgctctcat aagaaaggtc ggcaaggctg acgcaatctg cgtttactat acagccgaca
     7801 gacagattga gccaaatgaa attaagtctg agcttgcaaa agctctcacg gattatatgg
     7861 ttccgagcgc atatgttcag cttgacgaaa tgcctctcac accaaacggc aaggtcaaca
     7921 caaaggttct tcccgaacct aaaagcaaca aatccgaaag aggtcggttg ccgaagaatg
     7981 aacttgaaaa aacattctgc gacatctttg cagaaattct tgaacttgac aatgtttttg
     8041 cagacgataa cttctttgac ctcggcggaa catcgcttac agcaacaaga attgtaatct
     8101 ccgcatcaaa gaaaaatatt gaggttgcat acagcgacat ttttgcaaat cctactccgc
     8161 agactcttgc aaaatttgtg agcaaggatg attcagccga agacgacctt gaaaatcttt
     8221 cggactacga ttatacaaat atcaataaag ttcttgaaaa gaataatatt gatacattta
     8281 aaaacggtga gcttcaaaaa ctcggcaatg ttcttctgac aggctctgcc ggcttcctcg
     8341 gagtacacat tctctacgaa cttcttcaca agtataacgg caaggtttac tgcatgattc
     8401 gtgacaaaaa caataatcct gccgaaaaca gaatgaactc aatctactat tactactttg
     8461 aagaaagtct gaaagaaaga tatcccgaca gagttaccgt tatcagcggt gatgttacaa
     8521 accgtgaatc attcgataaa tttattgaca aggacattaa cacggtcatc aactgtgccg
     8581 caaatgttaa gcacttttca aagggtacag acattgaaga tgtaaacctt tacggtactc
     8641 tcaatgtgct tgacttctgc aaaaaggcta atgcaaggct tgttcatgtg tcaacaatga
     8701 gcgtaggcgg tatgtttgtg ggcgaacaag gttctgttga caagctcaaa gaaaatcaac
     8761 tttactttgg tcagcatgag ggttcaaagt acaccctgtc aaagttcctt gcggaaaggg
     8821 cgattcttga agaggtttca aagggcttta acgcaaagat tatgcgtgta ggcacacttg
     8881 ccgcaagaaa cagcgacggt gaatatcaga ttaatttcac caccaacaca tttatgggca
     8941 gactcaaatc tacactcctt atcggcaaat atccgtatga ggcaatggaa atgccgtttg
     9001 agctttctcc gattgatttt gtggcaaagg caattcttct gcttgcacag gctccgaagg
     9061 attgcaccgt gttccacccg ttcaacaacc atactctcat tatgggcgac ctctacaccg
     9121 agatgaacaa aatcggactt cattcacagg gtgcagagta tgaggaatat atgattgcac
     9181 ttgacagagc tgagcaggat cccgaaaaag caaagattct ttcgagtatg attgcatatc
     9241 agaacatggc tcacggtcaa aagacattta cagtcggcaa gagcaacaca tatacaatgc
     9301 aggttcttta cagaatggga tttgtgtggc ccgttacctc gcttgactat atgaaacgat
     9361 tcatcaacgc attaagaggt cttggtttct tcgatctcga gcaccaccac caccaccact
     9421 gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     9481 aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag
     9541 gaggaactat atccggat
//
