LOCUS       sequence-206079-        5377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal part of the split-Cre System.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5377)
  AUTHORS   Hirrlinger J, Scheller A, Hirrlinger PG, Kellert B, Tang W, Wehr MC,
            Goebbels S, Reichenbach A, Sprengel R, Rossner MJ, Kirchhoff F
  TITLE     Split-cre complementation indicates coincident activity of different
            genes in vivo.
  JOURNAL   PLoS One. 2009;4(1):e4286. doi: 10.1371/journal.pone.0004286. Epub 
            2009 Jan 27.
  PUBMED    19172189
REFERENCE   2  (bases 1 to 5377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009"; volume: "4(1):e4286. doi"; pages: " 
            10.1371/journal.pone.0004286. Epub 2009 Jan 27"
COMMENT     Sequence Label: pCMV-Tag3B-CCre
FEATURES             Location/Qualifiers
     source          1..5377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        64..367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        368..571
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     522..542
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        617..635
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      667..676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             676..705
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             790..846
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             919..939
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(1803..1819)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1804..1820)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(1875..1894)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1876..1894)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    2168..2289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2205..2224)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2259..2278
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2296..2751)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2433..2454)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2645..2664
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2778..2882
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2884..3241
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2884..3075
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2907..2927)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3092..3227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3276..4070
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3276..4070
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3330..3349)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3940..3959
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4258..4277)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4302..4349
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4678..5266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5167..5186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cattagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
       61 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      121 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      181 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      241 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      301 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      361 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      421 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      481 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      541 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
      601 attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggag ctccaccgcg
      661 gtggcggccg ccatggagca gaaactcatc tctgaagagg atctgagccc gggcggatcc
      721 aacactgaag ccgccaggcg ttctcgtgcg agaaagttgc aaagaatgaa acaacttgaa
      781 gacaaggttg aagaattgct ttcgaaaaat tatcacttgg aaaatgaggt tgccagatta
      841 aagaaattag ttggcgaacg cgccagccct agcaaccctg gcgccagcaa cggcagcctg
      901 caggaattca gcggccctcc caagaagaag aggaaggtga acaggaaatg gttccctgct
      961 gaacctgagg atgtgaggga ctacctcctg tacctgcaag ccagaggcct ggctgtgaag
     1021 accatccaac agcacctggg ccagctcaac atgctgcaca ggagatctgg cctgcctcgc
     1081 ccttctgact ccaatgctgt gtccctggtg atgaggagaa tcagaaagga gaatgtggat
     1141 gctggggaga gagccaagca ggccctggcc tttgaacgca ctgactttga ccaagtcaga
     1201 tccctgatgg agaactctga cagatgccag gacatcagga acctggcctt cctgggcatt
     1261 gcctacaaca ccctgctgcg cattgccgaa attgccagaa tcagagtgaa ggacatctcc
     1321 cgcaccgatg gtgggagaat gctgatccac attggcagga ccaagaccct ggtgtccaca
     1381 gctggtgtgg agaaggccct gtccctgggg gttaccaagc tggtggagag atggatctct
     1441 gtgtctggtg tggctgatga ccccaacaac tacctgttct gccgggtcag aaagaatggt
     1501 gtggctgccc cttctgccac ctcccaactg tccacccggg ccctggaagg gatctttgag
     1561 gccacccacc gcctgatcta tggtgccaag gatgactctg ggcagagata cctggcctgg
     1621 tctggccact ctgccagagt gggtgctgcc agggacatgg ccagggctgg tgtgtccatc
     1681 cctgaaatca tgcaggctgg tggctggacc aatgtgaaca ttgtgatgaa ctacatcaga
     1741 aacctggact ctgagactgg ggccatggtg aggctgctcg aggatgggga ctagaagctt
     1801 atcgataccg tcgacctcga gggggggccc ggtaccttaa ttaattaagg taccaggtaa
     1861 gtgtacccaa ttcgccctat agtgagtcgt attacaattc actcgatcgc ccttcccaac
     1921 agttgcgcag cctgaatggc gaatggagat ccaattttta agtgtataat gtgttaaact
     1981 actgattcta attgtttgtg tattttagat tcacagtccc aaggctcatt tcaggcccct
     2041 cagtcctcac agtctgttca tgatcataat cagccatacc acatttgtag aggttttact
     2101 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     2161 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     2221 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     2281 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     2341 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2401 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     2461 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     2521 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2581 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     2641 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     2701 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca
     2761 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     2821 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     2881 atcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     2941 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3001 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3061 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3121 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3181 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     3241 agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     3301 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     3361 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     3421 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     3481 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     3541 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     3601 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     3661 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     3721 aagccggtct tgtcgatcag gatgatctgg acgaagaaca tcaggggctc gcgccagccg
     3781 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg
     3841 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     3901 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     3961 ctgaagaact tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     4021 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     4081 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     4141 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     4201 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     4261 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     4321 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     4381 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     4441 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     4501 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     4561 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     4621 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     4681 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     4741 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     4801 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     4861 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     4921 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     4981 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5041 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5101 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5161 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5221 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5281 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     5341 atcccctgat tctgtggata accgtattac cgccatg
//
