LOCUS       sequence-20608-e         718 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 718)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG413GPD-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..718
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(44..168)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(176..192)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(176..192)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(198..461)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(281..304)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 ggaagcataa agtgtaaagc ctgggggtgc ctaatgcggc cgccatagtg actggatatg
       61 ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat
      121 atttatatca ttttacgttt ctcgttcagc ttttttgtac aaacttgtga tgggggatcc
      181 actagttcta gaatccgtcg aaactaagtt ctggtgtttt aaaactaaaa aaaagactaa
      241 ctataaaagt agaatttaag aagtttaaga aatagattta cagaattaca atcaatacct
      301 accgtcttta tatacttatt agtcaagtag gggaataatt tcagggaact ggtttcaacc
      361 ttttttttca gctttttcca aatcagagag agcagaaggt aatagaaggt gtaagaaaat
      421 gagatagata catgcgtggg tcaattgcct tgtgtcatca tttactccag gcaggttgca
      481 tcactccatt gaggttgtgc ccgttttttg cctgtttgtg cccctgttct ctgtagttgc
      541 gctaagagaa tggacctatg aactgatggt tggtgaagaa aacaatattt tggtgctggg
      601 attctttttt tttctggatg ccagcttaaa aagcgggctc cattatattt agtggatgcc
      661 aggaataaac tgttcaccca gacacctacg atgttatata ttctgtgtaa cccgcccc
//
