LOCUS       sequence-206127-        2739 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC16
FEATURES             Location/Qualifiers
     source          1..2739
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(596..614)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        682..786
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1008..1799
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1970..2558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2459..2478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2712..2729
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tcactctgtg
       61 gtctcaaatg atgtcaagtt ctggtgctcc ttcacgcaag actctgagta agatcgcgac
      121 taatcgtcta cagaaagagc ttgtggaatg gcagatgaat ccacctactg gtttcaaaca
      181 caaagtcact gataatctcc aaagatggat aattgaagtt attggagctc caggaactct
      241 atatgccaac gatacttatc agcttcaagt tgattttcca gaacattatc ctatggaatc
      301 gccacaagtg atttttcttc atccagctcc tctgcatcct cacatttaca gcaatgggca
      361 tatttgtcta gatattttgt atgattcgtg gtcaccagcc atgacggtga gttctatctg
      421 cattagcatc ctctccatgc tctcaagctc gactgaaaag caacgaccaa ccgataatga
      481 ccgatatgtg aagaattgta agaacggaag atctccaaag gaaacccgat ggtggttcca
      541 cgacgataaa gtataagctt tgagaccacg aagtggctct tcagtggacg aaagggcctc
      601 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
      661 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      721 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      781 aagagtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
      841 gcagtggcgg ttttcatggc ttgttatgac tgtttttttt tggggtacag tctatgcctc
      901 gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca
      961 acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg
     1021 tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg
     1081 aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac
     1141 acagcgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag
     1201 ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg
     1261 ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc
     1321 gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag
     1381 ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct
     1441 tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg
     1501 cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa
     1561 atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga
     1621 aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac
     1681 ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc
     1741 agttggaaga atttgtccat tacgtaaaag gcgagatcac caaggtagtc ggcaaataac
     1801 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1861 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1921 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1981 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2041 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2101 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2161 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2221 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2281 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2341 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2401 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2461 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2521 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2581 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2641 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2701 cgaccgagcg cagcgagtca gtgagcgagg aagcggaag
//
