LOCUS       sequence-206131-        2796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC19
FEATURES             Location/Qualifiers
     source          1..2796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     7..24
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(687..705)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        773..877
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1099..1890
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2061..2649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2550..2569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
       61 cccgcgcgtt ggccgattca ttaatcactc tgtggtctca aatggcgacg gttaatgggt
      121 acacagggaa tactccggcg gcgactacac cggcagccac cggatcaaag caatctgctc
      181 ctccgactaa gaccgtcgat agccactccg ttctaaaaag gctgcaatct gaactaatgg
      241 gattgatgat gggagctgat ccggggatat ctgcgtttcc agaggaggac aacatatttt
      301 gctggaaagg aacaattaca ggaagcaaag atactgtgtt cgaaggaact gagtacagac
      361 tctcactcac tttctctaac gattatcctt ttaagtctcc caaagttaag tttgagacat
      421 gctgcttcca ccccaatgtg gatctctatg gcaatatttg cttggacatt cttcaggata
      481 aatggtcatc tgcttatgat gtgaggacga tattactctc aattcaaagc cttctcggag
      541 aaccgaacat cagctcacca ttgaacaatc aagcggctca gctttggagc aatcaagaag
      601 agtacaggaa gatggttgag aagctctaca agcctttaaa cgcatgagct ttgagaccac
      661 gaagtggctc ttcagtggac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
      721 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
      781 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      841 ctgataaatg cttcaataat attgaaaaag gaagagtatg cgctcacgca actggtccag
      901 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
      961 ctgttttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc
     1021 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct
     1081 aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     1141 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     1201 ggctccgcag tggatggcgg cctgaagcca cacagcgata ttgatttgct ggttacggtg
     1261 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     1321 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     1381 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     1441 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     1501 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     1561 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     1621 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     1681 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     1741 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa
     1801 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ttacgtaaaa
     1861 ggcgagatca ccaaggtagt cggcaaataa ctgtcagacc aagtttactc atatatactt
     1921 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1981 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2041 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2101 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2161 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     2221 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2281 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2341 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2401 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2461 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2521 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2581 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2641 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     2701 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     2761 gagtgagctg ataccgctcg ccgcagccga acgacc
//
