LOCUS       sequence-206133-        2724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC21
FEATURES             Location/Qualifiers
     source          1..2724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     36..53
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(644..662)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        730..834
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1056..1847
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2018..2606
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2507..2526
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
       61 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatcactct
      121 gtggtctcaa atgcaggcat caagagcgcg actgtttaaa gaatacaaag aggtacagcg
      181 agagaaagta gcagatcctg atattcaatt gatctgtgac gataccaaca tattcaaatg
      241 gaccgctctt atcaaaggac cttcggagac tccttacgaa ggcggtgttt ttcagcttgc
      301 tttttctgtt cctgaacctt atcctttgca acctcctcaa gttcgattct tgaccaagat
      361 attccatcct aatgttcatt tcaagacagg agaaatatgt ctcgacattt tgaagaatgc
      421 ttggagtcct gcttggacgc ttcagtctgt gtgtagagct atcatagcat tgatggctca
      481 tcctgagccg gacagtcctc ttaactgcga ctcagggaat cttctaagat ctggtgatgt
      541 gagagggttc aattcaatgg cacaaatgta tacacgcctc gccgctatgc ctaagaaagg
      601 atgagctttg agaccacgaa gtggctcttc agtggacgaa agggcctcgt gatacgccta
      661 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
      721 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      781 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgcgc
      841 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
      901 ttcatggctt gttatgactg tttttttttg gggtacagtc tatgcctcgg gcatccaagc
      961 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     1021 agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg atcgccgaag
     1081 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     1141 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agcgatattg
     1201 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     1261 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     1321 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     1381 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     1441 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     1501 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     1561 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     1621 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     1681 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     1741 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     1801 ttgtccatta cgtaaaaggc gagatcacca aggtagtcgg caaataactg tcagaccaag
     1861 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1921 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1981 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2041 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2101 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2161 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2221 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2281 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2341 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2401 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2461 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2521 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2581 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2641 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2701 accgtattac cgcctttgag tgag
//
