LOCUS       sequence-206134-        3006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC22
FEATURES             Location/Qualifiers
     source          1..3006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(881..899)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        967..1071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1293..2084
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2255..2843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2744..2763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(2997..3006,1..8)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
       61 ttcattaatc actctgtggt ctcaaatggc tagtaatgag aatctaccac ccaatgttat
      121 caagcagctt gccaaggagc tgaagagtct tgacgaatcc cctccagatg gcatcaaggt
      181 tgtggtaaat gacgaggatt tctcacaaat atgtgctgat attgaaggac cagttggtac
      241 tccttatgag aacggacttt tccgtatgaa gttggccttg tcacatgatt ttccacattc
      301 gcctcccaaa ggctacttca tgacaaagat tttccatcct aatgttgctt ctaatggaga
      361 gatatgtgtt aacacgctta aaaaagattg gaaccctagt cttggattaa gacatgttct
      421 ctctgttgta aggtgcttat taatcgagcc atttccagaa tctgcattaa atgaacaagc
      481 tggcaagatg ctacttgaaa actatgacga gtatgcgagg catgctaggc tttacacagg
      541 tatccatgct aaaccaaaac ccaagttcaa gacaggagca atctcagagt caacaaccgc
      601 tctaaatgtt ggccagacga acaacgagac gcctggtgct gctactgcaa taccctcttc
      661 aatgacagac ataaagagag taacaacgag tgcccaagac caacaacacg tagctaatgt
      721 cgttgtagca gcctctgcaa gtgtggtcac tacgacgcaa aaaagggaag caggtttggc
      781 caaggttcag gcagataaga agaaggtaga cgctagaaag aaaagcttga agagactatg
      841 agctttgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat acgcctattt
      901 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
      961 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1021 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgcgctca
     1081 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     1141 atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca tccaagcagc
     1201 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
     1261 agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc gccgaagtat
     1321 cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg
     1381 ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc gatattgatt
     1441 tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg atcaacgacc
     1501 ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta gaagtcacca
     1561 ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa ctgcaatttg
     1621 gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg atcgacattg
     1681 atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta ggtccagcgg
     1741 cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta aatgaaacct
     1801 taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta gtgcttacgt
     1861 tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg
     1921 actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa gctagacagg
     1981 cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg
     2041 tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca gaccaagttt
     2101 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     2161 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     2221 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     2281 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     2341 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     2401 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     2461 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     2521 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     2581 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     2641 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     2701 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     2761 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2821 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2881 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2941 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3001 agtcag
//
