LOCUS       sequence-206137-        4080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC25
FEATURES             Location/Qualifiers
     source          1..4080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1919..1937)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2005..2109
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2331..3122
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3293..3881
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3782..3801
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4035..4052
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatcactctg tggtctcaaa tggagcccaa
       61 cgtagtggag atcgctactc ctcctgccgc ttcttgctcg agaattcgaa cgccaacaaa
      121 ggctgaaact ccagaagtga ttgatgtgga ggaatatgat ttgcaaaacg gtggtgttcc
      181 taatggcaac aatgttgatt ataagaacaa agggaaggct atagactttg attccatgtc
      241 ttacggtgac tatggtgaag aagatgagta tgctgtggga tctcctggtg atgattatgg
      301 ttatcctgaa tcatctccac tatcaaatag tttgttagac ccagagtctt tgatctatga
      361 ggacgacgaa aactattccg aacaatatga ctttgaaatg gaggcggagc ctgataatta
      421 ctccatgtat caagaccttt tcgatggtaa ggatatccca actggtgtgg aggtttcaat
      481 ggattggttt ccaaattcag ctgacaagga atcagcgagt agtagcaaat cgtctcatgc
      541 taataacggg aataacagtt ccaagaaagc aacaaaagca agtggaatcc attctcaatt
      601 ttcttcagat atggaaaccc ctgtagctca accatggaat gctttaccgc acaaagctga
      661 aggagtcata ccaaactctg cttatgcctt gccacagaac agcaaggctt ttcaaccacc
      721 ttacgctgtc cactattcag cccttaaaac tgctttttcc aattatcttc agcctcagac
      781 tcctgatacc gtattggggg aggctccagc tcctgctgca ggttcttctg gtttattgcc
      841 accaaacaca cccggtttta aaagtaatgc tgctcggttt aaagaggagc cgcctatatt
      901 acctccggat gattccagag ttaagagaaa tatggaggat tatcttgggc tttatttgtt
      961 tttcaaaaga tttgacatcg tcgaagattt ttcagatcac cactatgctt ctaagggaac
     1021 aacttcaaag cagcattcaa aggattgggc gaaaaggatc caagacgagt ggagaattct
     1081 tgagaaagat ttaccagaga tgatatttgt cagagcttat gaatctagga tggatctttt
     1141 aagggctgta attatcggag cccaaggaac tccctaccat gatggactct tcttttttga
     1201 tatcttcttc ccggatacat acccttctac gccaccgatt gttcattacc attctggtgg
     1261 gctaagaatc aacccaaatc tgtacaattg tgggaaagtg tgtctgagtc tactcggcac
     1321 ttggagtggt aaccagaggg agaaatggat ccccaacact tcgacgatgc tacaagttct
     1381 tgtctcgatc caaggtctaa tcttaaatca aaaaccttac ttcaacgagc ccggctacga
     1441 gaggtcagca ggttctgcac atggtgagag tacctctaaa gcttacagcg aaaacacatt
     1501 catactgtct ttaaagacta tggtttacac catgaggagg ccacccaagt attttgagga
     1561 ctttgcgtat ggacatttct tcagctgtgc tcacgacgtg ttgaaggcgt gtaatgctta
     1621 cagaaacgga gctacaccgg gatatctggt gaagggggct ccagatgtgg aagagaatag
     1681 cgcgggcatg agctctctga agtttaggac agatgtggct acctttgtgg agacagtgct
     1741 gttgaaagag tttattcttc taggtgtcct tggactcgaa ccggaagaag aagaaaaaac
     1801 acctgagact ataatcgttg cagagagtag caaatgtacc agatcaaggt ccaagagaga
     1861 cagagtttct tctagttgag ctttgagacc acgaagtggc tcttcagtgg acgaaagggc
     1921 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     1981 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     2041 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     2101 aggaagagta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac
     2161 ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttttggggta cagtctatgc
     2221 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     2281 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgagggaag
     2341 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2401 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     2461 cacacagcga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     2521 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     2581 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     2641 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     2701 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     2761 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     2821 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     2881 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     2941 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     3001 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     3061 atcagttgga agaatttgtc cattacgtaa aaggcgagat caccaaggta gtcggcaaat
     3121 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3181 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3241 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3421 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3901 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3961 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     4021 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
//
