LOCUS       sequence-206138-        3882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning Module.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-UBC26
FEATURES             Location/Qualifiers
     source          1..3882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     132..151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     385..402
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2151..2169)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2237..2341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2563..3354
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3525..3882,1..231)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
       61 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      121 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      181 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      241 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      301 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      361 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      421 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatcactctg tggtctcaaa
      481 tggagcctga cgtggtggag atccctcctc ctcctctgat cgcttcgggt tctagaactc
      541 gtaaacccag aaaggctgtt cctgaagtga ttgatgtgga aagttatgaa ttccgcaatg
      601 ttggtgttgt taaggataac aatgttgttg ataagaagaa caaagggaag gcaatacaag
      661 ttgactcctt ttctttcaac aacgttcaat ctcatcatca tggatcgtcg ctgcttaact
      721 tagagacgtt ccaagactat tatggtcata agaatatccc ttttagtgaa tttgctaatc
      781 aaccaattga tgttgatgac tactcaatgt atcaagacgt tctagatcct aaggatgttc
      841 cagctggtgc agaggtgacg gtaccttggg gtttaaattc gagcagtaag ggaactgcga
      901 aatcaagtat aagcattatg agaagtcaat ccatgaaagg atatggaaca gtatctctgg
      961 caacgactaa tgttcctcaa ttatgggatt atactttgcc tcagcagaat caggctatct
     1021 attcttctgt atcgttttct gcggttcaac ctcagactcc tgatgtagtg atggtcacca
     1081 atcctactcc aaaccctttt agttatgatg cttctgctag ttcttctcat cctatagcgg
     1141 ctgagcctat ttcttcagtt caggattctt ccaatgctag gaaacttaag gaagagtttc
     1201 tgagggattt taaaagattt gacaccgtcg aggatttctc agatcatcac tatgcttcaa
     1261 agggtaaatc ttcaaagcag cactcgaaga attgggtgaa aaaggttcaa gcagattgga
     1321 agattcttga gaatgatttg ccagaggcaa tatctgtcag agcctgtgaa tcaagaatgg
     1381 atcttttgag agctgtaatt attggagctg agggaactcc ttaccatgac ggtcttttct
     1441 tcttcgatat tcagttccca gatacttatc cttcagtgcc accgaatgtt cattaccatt
     1501 ctggtgggct tagaatcaac ccgaatctat acaattgtgg gaaagtatgc ttgagtctac
     1561 tcggtacctg ggcaggtagc gcgagggaga aatggctccc aaatgagtcg acaatgttac
     1621 agcttcttgt ctcaatccaa gcactcatct tgaatgaaaa accatacttt aacgaacctg
     1681 gctacgtaca gagcgcgggg actgcatcag gcgagtccaa gtctaaagtt tacagtgaga
     1741 acgtatttct attgtcgtta aagactatgg tttacagcat aagaaggcca ccccagcatt
     1801 ttgaagagta tgttcaaaac cattactttg tccgctctca cgacattgtg aaagcatgca
     1861 atgcttataa agctggagct ccgcttggat cgatggttaa aggcggagtc caagaccttg
     1921 aagaagctag acagagcggc tccaagaagt ttaagaccga tgtagctagc tttatgcaga
     1981 cagtggttga tgaattcgtc aaactaggag tcaaggagct tgcagaaaaa ccagaaccgc
     2041 ctatgagcaa tgctaacaca gagaatcaga gcaagaaaaa aacccgtaag agaagcagat
     2101 catcgaggtg agctttgaga ccacgaagtg gctcttcagt ggacgaaagg gcctcgtgat
     2161 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     2221 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     2281 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     2341 tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt
     2401 ggcggttttc atggcttgtt atgactgttt ttttttgggg tacagtctat gcctcgggca
     2461 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     2521 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca tcatgaggga agcggtgatc
     2581 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     2641 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagc
     2701 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     2761 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2821 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     2881 ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga gccagccacg
     2941 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     3001 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     3061 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     3121 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     3181 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     3241 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     3301 gaagaatttg tccattacgt aaaaggcgag atcaccaagg tagtcggcaa ataactgtca
     3361 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3421 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     3481 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     3541 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     3601 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     3661 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     3721 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     3781 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     3841 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cg
//
