LOCUS       sequence-20614-e         633 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG414GPD-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..633
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(33..157)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(165..181)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(165..181)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(187..450)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(270..293)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 gtgtaaagcc tgggggtgcc taatgcggcc gccatagtga ctggatatgt tgtgttttac
       61 agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat
      121 tttacgtttc tcgttcagct tttttgtaca aacttgtgat gggggatcca ctagttctag
      181 aatccgtcga aactaagttc tggtgtttta aaactaaaaa aaagactaac tataaaagta
      241 gaatttaaga agtttaagaa atagatttac agaattacaa tcaataccta ccgtctttat
      301 atacttatta gtcaagtagg ggaataattt cagggaactg gtttcaacct tttttttcag
      361 ctttttccaa atcagagaga gcagaaggta atagaaggtg taagaaaatg agatagatac
      421 atgcgtgggt caattgcctt gtgtcatcat ttactccagg caggttgcat cactccattg
      481 aggttgtgcc cgttttttgc ctgtttgtgc ccctgttctc tgtagttgcg ctaaaagaat
      541 ggacctatga actgatggtt ggtgaagaaa acaatatttt ggtgctggga ttcttttttt
      601 ttctggatgc cagcttaaaa agcgggctcc att
//
