LOCUS       sequence-206145-        4881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lenti-viral expression of Flag-tagged Depdc5 mutant B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Shen K, Huang RK, Brignole EJ, Condon KJ, Valenstein ML, 
            Chantranupong L, Bomaliyamu A, Choe A, Hong C, Yu Z, Sabatini DM
  TITLE     Architecture of the human GATOR1 and GATOR1-Rag GTPases complexes.
  JOURNAL   Nature. 2018 Apr 5;556(7699):64-69. doi: 10.1038/nature26158. Epub 
            2018 Mar 28.
  PUBMED    29590090
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature26158"; journalName: "Nature"; date: "2018-04-5- 5"; 
            volume: "556"; issue: "7699"; pages: "64-69"
COMMENT     Sequence Label: [pSK481] pLJC5 Flag-Depdc5(B)
FEATURES             Location/Qualifiers
     source          1..4881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..33
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atgggctacc cctatgatgt gcctgactac gcagatctca atggtggtgg tggtgggtcg
       61 acgatgagaa caacaaaggt ctacaaactc gtcatccaca agaagggctt tgggggcagt
      121 gatgatgagc tagttgtgaa ccccaaagtg ttccctcaca tcaagcttgg agacattgta
      181 gagattgcac accccaacga tgaatacagc cctctgcttt tgcaggtcaa gtctcttaag
      241 gaagatttac agaaggaaac tatcagtgtg gaccagactg tgactcaagt gttccggctg
      301 agaccttatc aggatgtcta tgttaatgtc gtagacccta aggatgtgac ccttgaccta
      361 gtggaattaa cttttaagga tcagtatatt ggccgtgggg atatgtggcg actaaagaaa
      421 agtttggtca gcacatgtgc ctatatcacc cagaaggtgg agtttgctgg catcagagca
      481 caggctggtg aactgtgggt taagaatgag aaggtcatgt gtggctacat cagtgaagat
      541 accagggtgg tgtttcgttc tacgtcggct atggtttaca tatttattca gatgagctgt
      601 gaaatgtggg attttgatat ttatggggat ttgtattttg agaaagctgt gaatggtttc
      661 cttgctgatc tatttaccaa gtggaaggag aagaactgta gtcatgaagt gacagtggtc
      721 ctgttttcta gaactttcta tgatgcaaaa tctgttgatg aatttcctga aataaaccga
      781 gcctcaattc gacaggatca caaggggaga ttctatgaag acttttacaa agtggtggtg
      841 cagaatgaga gaagagaaga atggacttca cttctcgtaa ccattaaaaa actcttcatc
      901 cagtatccag tgttggtgcg actggaacag gcagagggct ttcctcaagg agataattct
      961 acctcagcac aaggaaacta cctggaggcc atcaatctgt cattcaatgt gtttgataag
     1021 cactacatca accgcaactt tgaccgaact gggcagatgt cagtggtgat cacgcccggg
     1081 gtgggtgtct ttgaagtgga ccgcctactc atgatcctga ccaagcagcg gatgatagat
     1141 aatggaattg gtgtggattt ggtgtgcatg ggagagcaac cgttacatgc tgtcccattg
     1201 ttcaagctcc ataatcggag tgctccccgt gattctcgtc tgggcgatga ctataatatc
     1261 cctcactgga taaaccacag tttctacaca tccaaaagcc agctcttttg taatagtttc
     1321 accccacgaa taaaactggc aggaaagaag cccgcctctg agaaagcaaa aaatggccgt
     1381 gatacatctc tcgggagtcc aaaagaatct gagaacgccc ttcccatcca agtagattat
     1441 gacgcctatg acgctcaagt gttcaggctg cccggcccat cccgggccca gtgcctcacc
     1501 acctgcagat ctgtgcgaga gcgagagagt cacagtcgaa agagtgccag ctcctgtgat
     1561 gtttcatcca gcccttccct accaagccgc acactgccca ctgaggaagt gaggagccag
     1621 gcttctgacg acagctccct aggcaagagt gccaacatcc tgatgatccc acacccccac
     1681 ctgcaccagt atgaagtcag cagctccttg ggatacacca gcactcgaga tgtcctggag
     1741 aacatgatgg agccaccaca gcgagactcc agtgcaccag ggaggtttca cgttggcagt
     1801 gcagaatcca tgctgcatgt tcgacctggt ggatacacgc cccagagagc actgattaac
     1861 cccttcgctc cctctcggat gcccatgaag cttacgtcca acagaaggcg ctggatgcac
     1921 acttttcctg tggggccatc cggagaagcc atccagatcc accaccagac ccgacagaat
     1981 atggcggagc tacaaggcag cgggcagagg gatccaactc actcctctgc agagctgctg
     2041 gagttagcat atcatgaagc tgctggaagg cacagcaatt cccgccagcc tggtgacggc
     2101 atgtccttct tgaacttcag tggaacagag gagctttctg tcggcctgct tagcaacagt
     2161 ggtgcaggta tgaatcctag gacccagaat aaggattctc tagaggacag tgtttctacc
     2221 tctccagacc caattctgac actgtctgct ccccctgtag tgccaggctt ctgttgcaca
     2281 gttggagtgg actggaagtc tctcactact ccggcgtgcc tcccccttac caccgactac
     2341 ttccctgacc gccagggcct gcagaatgac tacacagagg gctgttatga tctccttcca
     2401 gaagcagaca tcgacaggag ggacgaagat ggtgtgcaga tgacagccca gcaggtattt
     2461 gaagagttta tttgccaacg tctcatgcag ggctaccaaa tcatagtgca gcccaagaca
     2521 cagaaaccca atcctgctgt cccgcccccg ctgagcagta gcccactcta tagccgaggc
     2581 cttgtgtccc gaaaccgccc tgaggaggag gaccagtatt ggctgagtat gggcagaacg
     2641 ttccacaaag tgacgctgaa ggataagatg atcacagtga cgcgatacct tcccaagtat
     2701 ccttatgaat ctgcccagat ccactacacc tacagcctct gtccttccca ctcagactca
     2761 gagttcgtct cctgctgggt ggagttttcc cacgaacggc tggaggagta caagtggaat
     2821 tacttagatc agtatatctg ttctgccggc tctgaagact tcagcttaat tgagtccctg
     2881 aagttctgga ggacccgctt cctgctgctg ccagcctgtg tcaccgccac caagcgcatc
     2941 acggaggggg aggcccactg cgacatctat ggggacaggc cccgtgcaga cgaggacgag
     3001 tggcaactcc tggatggttt tgtccgcttt gtggagggct tgaatcgcat tcgcaggcgg
     3061 catcgctcgg atcgcatgat gcggaaaggg accgccatga aaggcttgca gatgactggg
     3121 cccatttcca cgcattctct ggagtcaact gcacccccag tggggaagaa gggaacctca
     3181 gctctctctg ccctgttgga gatggaggcc agtcagaagt gcctgggaga acagcaggca
     3241 gctgtgcatg gtgggaagag ctccgcccag tcagccgaga gcagcagcgt tgccatgact
     3301 cccacctaca tggacagccc acgaaaggac ggggccttct ttatggagtt tgtccgcagc
     3361 ccacgcacag catcgtccgc cttctaccct caggtatctg tggaccaaac agccactcct
     3421 atgttggacg gcaccagttt gggcatatgc acaggccaat ccatggacag aggcaacagc
     3481 cagacctttg ggaactccca gaacatagga gaacagggct actcctccac aaactccagt
     3541 gacagcagct ctcagcagct ggtggcaagc tccttgacct catcctctac cctgacagag
     3601 atcctggaag ccatgaagca cccctcgaca ggagtccagc tgctctctga acagaagggc
     3661 ctctcaccgt actgcttcat cagcgcggag gtggtacact ggttggtgaa ccacgtggag
     3721 gggatccaga cacaggcgat ggccattgac atcatgcaga aaatgctgga agagcagctc
     3781 atcacacatg catctggcga agcctggcgg accttcatct acggcttcta tttctacaag
     3841 atagtaacgg acaaagagcc cgaccgagtg gccatgcagc agcccgccac cacctggcac
     3901 acagcaggag tggacgactt cgccagcttc cagcgcaagt ggtttgaggt ggcctttgtg
     3961 gcagaagagc tcgtgcactc tgagattcct gcctttctcc tgccctggct gcctagccgg
     4021 ccagcctcct atgcaagtag gcacagctcc tttagccgaa gttttggagg acggagccag
     4081 gcggcagcac ttttagctgc cactgtccca gagcagagga ctgtgaccct ggatgttgac
     4141 gtgaacaacc gcacagaccg gctggagtgg tgcagctgtt attaccatgg caacttttct
     4201 ctgaatgcag cctttgagat caagctgcac tggatggcgg tgaccgcagc agtactcttc
     4261 gagatggtcc aaggttggca tcggaaagcc acctcctgtg gcttcttgtt agtcccagtt
     4321 ttggaggggc cttttgcact gcccagttac ctgtatggcg acccccttcg tgcccagctc
     4381 ttcatcccac tcaacatcag ctgcttgctc aaggagggca gcgagcacct gtttgatagc
     4441 tttgaacccg aaacgtactg ggatcgaatg cacctcttcc aggaagccat tgcacacagg
     4501 tttgggtttg tacaagataa atattctgcc tctgctttta acttccctgc tgagaacaag
     4561 cctcagtata tccacgttac aggaacagtg tttctgcagc tgccctactc caagcgcaag
     4621 ttctcagggc agcagcggcg gcggcggaac tccaccagct ccaccaacca gaacatgttc
     4681 tgcgaggagc gggtcggcta caactgggcc tacaacacca tgctcaccaa aacatggcgc
     4741 tccagcgcca caggggatga aaagtttgct gatcggctgc tgaaggactt cacggacttc
     4801 tgcatcaacc gtgacaaccg gctggtcacg ttctggacaa gttgcctgga gaagatgcat
     4861 gccagtgccc cgtgagaatt c
//
