LOCUS       sequence-206162-       12373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  virus packaged, doxycyline inducible expression of Cdt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12373)
  AUTHORS   Matson JP, Dumitru R, Coryell P, Baxley RM, Chen W, Twaroski K, 
            Webber BR, Tolar J, Bielinsky AK, Purvis JE, Cook JG
  TITLE     Rapid DNA replication origin licensing protects stem cell 
            pluripotency.
  JOURNAL   Elife. 2017 Nov 17;6. doi: 10.7554/eLife.30473.
  PUBMED    29148972
REFERENCE   2  (bases 1 to 12373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-11-17"; volume: "6. doi"; pages: " 10.7554/eLife.30473"
COMMENT     Sequence Label: pInducer20-Blast-CDT1-HA
FEATURES             Location/Qualifiers
     source          1..12373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             67..93
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        179..1390
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1227..1246
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(1449..2066,2067..2072,2073..2108,2109..2111,2112..2147,
                     2148..2150,2151..2186,2187..2195)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    2233..2807
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2400..2417)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             2817..3215
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             3226..4020
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3226..4020
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3280..3299)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3890..3909
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    4199..4787
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4252..4272)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4670..4681)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4862..5095
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5214..5232)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5300..5404
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5405..5473,5474..6265)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5623..5642)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6436..7024
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6925..6944
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7178..7195
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7435..7456
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7471..7476,7477..7494,7495..7501)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7509..7525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7514..7536
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7533..7549
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7533..7549
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7599..7618
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7810..8443
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8490..8615
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9112..9345
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9530..9574
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9867..9984
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(10039..10057,10058..10080,10081..10099,10100..10122,
                     10123..10141,10142..10164,10165..10183,10184..10206,
                     10207..10225,10226..10248,10249..10267,10268..10290,
                     10291..10309)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        10342..10380
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10377..10401
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    10510..10534
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10666..10938
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             12217..12243
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             12247..12273
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             12283..12306
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             12310..12339
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             12313..12342
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             12316..12345
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(join(12363..12373,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg
       61 ttcgaatacc catacgacgt cccagactac gcttagtaat gattaattaa actagaaatt
      121 ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gtaacccacc caagatctgg
      181 cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagcgctgcc
      241 acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct caggacagcg
      301 gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga cattttagga
      361 cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac aggcgaggaa
      421 aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga acgccgatga
      481 ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg atttgggtcg
      541 cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct gctgggctgg
      601 ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga gagaccgcca
      661 agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg ggggagcgca
      721 gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg ggctgtgagg
      781 tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt gaggccttcg
      841 ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg gggaccctga
      901 cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg gcggcagtta
      961 tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc gtcgtgtcgt
     1021 gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg taggtgtgcg
     1081 gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc tcctgaatcg
     1141 acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc tttggtcggt
     1201 tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc gctcggggtt
     1261 ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat
     1321 atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt
     1381 tttgttagac gaagcttggt accgagctcg gatctccacc ccgtaccggt cctgcagtcg
     1441 aattcaccat gtctagactg gacaagagca aagtcataaa cggagctctg gaattactca
     1501 atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc
     1561 agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg
     1621 agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact
     1681 ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta
     1741 aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg
     1801 cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg
     1861 gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa
     1921 gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg
     1981 accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg
     2041 agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag
     2101 acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc
     2161 ttgacgattt tgaccttgac atgctccccg ggtaactaag taaggatccg cggccgcact
     2221 agaggaattc cgcccctctc cctccccccc ccctaacgtt actggccgaa gccgcttgga
     2281 ataaggccgg tgtgtgtttg tctatatgtt attttccacc atattgccgt cttttggcaa
     2341 tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg gtctttcccc
     2401 tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc
     2461 ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc ccccacctgg
     2521 cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa aggcggcaca
     2581 accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc tctcctcaag
     2641 cgtagtcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg gaatctgatc
     2701 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     2761 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct taaaccatgg
     2821 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
     2881 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca
     2941 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
     3001 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
     3061 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
     3121 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
     3181 aattgctgcc ctctggttat gtgtgggagg gctaaccggt ccaccatgat tgaacaagat
     3241 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3301 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3361 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     3421 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     3481 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3541 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3601 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3661 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     3721 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     3781 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     3841 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     3901 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     3961 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4021 tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     4081 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     4141 aattgttgtt gtttagtccc tcccaattcg atatcaagct tatcgatcga tagatcctaa
     4201 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     4261 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     4321 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     4381 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     4441 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     4501 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     4561 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     4621 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     4681 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4741 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg agatccttta
     4801 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4861 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     4921 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4981 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5041 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     5101 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     5161 gagaggcccg ggttaattaa ggaaagggct agatcattct tgaagacgaa agggcctcgt
     5221 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     5281 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5341 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     5401 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5461 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5521 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5581 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5641 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5701 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5761 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5821 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5881 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5941 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6001 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6061 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6121 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6181 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6241 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     6301 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     6361 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6421 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6481 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6541 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6601 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6661 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6721 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6781 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6841 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6901 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6961 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7021 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7081 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7141 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7201 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     7261 caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7321 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     7381 cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     7441 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     7501 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     7561 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7621 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     7681 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     7741 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     7801 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     7861 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     7921 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     7981 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     8041 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     8101 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     8161 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     8221 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     8281 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     8341 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     8401 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     8461 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     8521 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     8581 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     8641 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     8701 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     8761 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     8821 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     8881 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     8941 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     9001 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     9061 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     9121 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     9181 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     9241 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     9301 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     9361 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     9421 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     9481 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     9541 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     9601 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9661 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9721 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
     9781 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccgaat
     9841 tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt
     9901 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     9961 caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg
    10021 actaggatcc tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
    10081 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
    10141 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
    10201 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
    10261 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
    10321 cgagctcggt acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct
    10381 cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga
    10441 agacaccggg accgatccag cctccgcggc cccgaactag tccagtgtgg tggaattctg
    10501 cagatatcaa caagtttgta caaaaaagca ggctctttaa aggaaccaat tcagtcgact
    10561 ggatccggta ccgaattcat ggagcagcgc cgcgtcaccg acttcttcgc gcgccgccgc
    10621 cccgggcccc cccgcatcgc gccgcccaag ctggcctgcc gcacccccag ccccgccagg
    10681 cccgcactcc gcgccccggc ctccgctacc agtggcagcc gcaagcgcgc ccgcccgccc
    10741 gccgcccccg gacgcgacca ggccaggcca ccggcccgca ggagactgcg gctgtcggtg
    10801 gacgaggttt ccagccccag tacccccgag gccccagaca tcccagcctg cccttctccg
    10861 ggccagaaga taaagaaatc caccccggca gcaggtcagc cgccccacct gacatccgcg
    10921 caggaccagg acaccatctc tgagcttgcg tcatgcctgc aacgggcccg ggagctgggg
    10981 gcaagagtcc gggcgctgaa ggccagtgcc caggatgctg gggagtcctg caccccagag
    11041 gccgagggcc gccctgagga gccatgtggc gagaaggcgc ccgcctacca gcgcttccat
    11101 gccctggccc agcccggcct gccgggactc gtgctgccct acaagtacca ggtgctggcg
    11161 gagatgttcc gcagcatgga caccatcgtg ggcatgctcc acaaccgctc cgagacgccc
    11221 acctttgcca aggtccagcg gggcgtccag gacatgatgc gtaggcgttt tgaggagcgc
    11281 aatgttggcc agatcaaaac cgtgtacccg gcctcctacc gcttccgcca ggagcgcagt
    11341 gtccccacct tcaaggatgg cgccaggagg tcagattacc agctcaccat cgagccactg
    11401 ctggagcagg aggctgacgg agcagccccc cagctcacgg cctcgcgcct cctgcagcga
    11461 cggcagatct tcagccagaa gctggtggag cacgtcaagg agcaccacaa ggccttcctg
    11521 gcctccctga gccccgccat ggtggtgccg gaggaccagc tgacccgctg gcacccgcgc
    11581 ttcaacgtgg atgaagtacc cgacatcgag ccggccgcgc tgccccagcc acccgccacg
    11641 gagaagctca ccactgctca ggaggtgctg gcccgggccc gcaacctgat ttcacccagg
    11701 atggagaagg ccttgagtca attggccctg cgctctgctg cgcccagcag ccccgggtct
    11761 cccaggccag cactgccggc taccccacca gccaccccgc ctgcagcctc tcccagtgct
    11821 ctgaaggggg tgtcccagga tctgctggag cggatccgag ccaaggaggc acagaagcag
    11881 ctggcacaga tgacgcggtg cccggagcag gagcagcggc tgcagcgctt agaacggctg
    11941 cctgagctgg cccgcgtgct gcggagcgtc tttgtgtccg aacgcaagcc tgcgctcagc
    12001 atggaggtgg cctgtgccag gatggtgggc agctgttgta ctatcatgag ccctggggaa
    12061 atggagaagc acctgctgct cctctccgag ctgctgccgg actggctcag cctccaccgc
    12121 atccgcaccg acacatatgt gaaactcgat aaggccgcgg acctggccca catcactgca
    12181 cgcctggccc accagacacg tgctgaggag gggctgtacc catacgatgt tcctgactat
    12241 gcgggctatc cctatgacgt cccggactat gcaactagtg cctggagtca ccctcagttc
    12301 gagaaagccc atcaccacca tcatcaccat caccaccacc atcattgact cgagatatct
    12361 agacccagct ttc
//
