LOCUS       sequence-20617-e         658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG415GAL-ccdB-EYFP
FEATURES             Location/Qualifiers
     source          1..658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(43..167)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(175..191)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(175..191)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(206..647)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(212..235)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    530..647
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 ggaagctaaa gtgtaaagcc tgggggtgcc taatgcggcc gccatagtga ctggatatgt
       61 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
      121 tttatatcat tttacgtttc tcgttcagct tttttgtaca aacttgtgat gggggatcca
      181 ctagttctag aatccggggt tttttctcct tgacgttaaa gtatagaggt atattaacaa
      241 ttttttgttg atacttttat tacatttgaa taagaagtaa tacaaaccga aaatgttgaa
      301 agtattagtt aaagtggtta tgcagttttt gcatttatat atctgttaat agatcaaaaa
      361 tcatcgcttc gctgattaat taccccagaa ataaggctaa aaaactaatc gcattatcat
      421 cctatggttg ttaatttgat tcgttcattt gaaggtttgt ggggccaggt tactgccaat
      481 ttttcctctt cataaccata aaagctagta ttgtagaatc tttattgttc ggagcagtgc
      541 ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga agacgaggac gcacggagga
      601 gagtcttcct tcggagggct gtcacccgct cggcggcttc taatccgtac tagagctc
//
